FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kibra, Mer
FlyBase ID
FBig0000095536
Interaction Network
Interactions Browser links
kibra network
Mer network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210017-1.coIP.WB
Reference
Yu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kibra 

Kibra

bait

T7 tag

Mer 

Mer

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged kibra and Mer constructs.

(Yu et al., 2010)
FBrf0210017-2.coIP.WB
Reference
Yu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

FLAG tag

kibra 

Kibra

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged kibra and Mer constructs.

(Yu et al., 2010)

Reaction is potentiated if tagged ex-HA construct also present in cell line.

(Yu et al., 2010)
FBrf0210017-3.coIP.WB
Reference
Yu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kibra 

Kibra

bait
Mer 

Mer

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0210061-1.coIP.WB
Reference
Baumgartner et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
kibra 

Kibra

bait

HA tag

Mer 

Mer

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Baumgartner et al., 2010)

Mer interacts with a truncated form of kibra that is missing the WW domain.

(Baumgartner et al., 2010)
FBrf0210061-2.coIP.WB
Reference
Baumgartner et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

HA tag

kibra 

Kibra

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Baumgartner et al., 2010)

Mer interacts with a truncated form of kibra that is missing the WW domain.

(Baumgartner et al., 2010)
FBrf0211094-2.coIP.WB
Reference
Genevet et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
kibra 

Kibra

bait

Myc tag

Mer 

Mer

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ cell line transfected with tagged kibra and Mer constructs.

(Genevet et al., 2010)
FBrf0248186-6.coIP.WB
Reference
Tokamov et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kibra 

Kib

bait

GFP tag

kibra 
Mer 

Mer

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kibra 
WW domains
mutation increasing interaction
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tokamov et al., 2021)
FBrf0259436-5.coIP.WB
Reference
Li et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kibra 

kib

bait

Fg tag

Mer 

Mer

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK-293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2024)

Addition of TER94 decreases Mer-kibra interaction. Addition of ubiquitin increases Mer-kibra interaction.

(Li et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)