Grip128, γTub23C Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Grip128, γTub23C

FlyBase ID

FBig0000097656

Interaction Network

Interactions Browser links

Grip128 network
γTub23C network

esyN Network Diagram

Reported Interactions

FBrf0107918-2.coIP.MW

Reference

Oegema et al., 1999

Description

physical association

Assay

anti bait coimmunoprecipitation

cosedimentation through density gradient

molecular weight estimation by staining

Collection

Source/Stage

organism / embryonic stage 1 embryonic stage 8 blastoderm stage embryonic stage 5 embryonic stage 3 embryonic cycle 6 embryonic cycle 8 embryonic cycle 5 embryonic stage 6 embryonic cycle 7 embryonic cycle 4 embryonic cycle 3 embryonic stage 4 embryonic stage 7 embryonic stage 2 embryonic cycle 9

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub23C

γtub

bait

Grip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oegema et al., 1999)

γTub23C and Grip128 coimmunoprecipitate and cosediment as part of the γTuRC (γ-tubulin ring complex).

(Oegema et al., 1999)

FBrf0201798-13.CS.WB

Reference

Wiese, 2008

Description

physical association

Assay

cosedimentation through density gradient

western blot

Collection

Source/Stage

Cell line used

Kc-unspecified

Participants

Corresponds to

Reported as

Role

Note

γTub23C

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0201798-22.CoIP.WB

Reference

Wiese, 2008

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

γTub23C

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0201798-25.CoIP.WB

Reference

Wiese, 2008

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub23C

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly linee; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0201798-3.CS.WB

Reference

Wiese, 2008

Description

physical association

Assay

cosedimentation through density gradient

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

γTub23C

bait

Grip128

Dgrip128

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)

FBrf0219801-30.CS.WB

Reference

Gopalakrishnan et al., 2012

Description

physical association

Assay

cosedimentation through density gradient

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub23C

γ-tubulin

neutral component

γTub23C

γ-tubulin

neutral component

γTub23C

γ-tubulin

neutral component

Grip128

neutral component

Grip128

neutral component

Grip128

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2012)

γTub23C and Grip128 co-fractionate as part of a Sas-4-containing S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)

FBrf0251596-10.Y2H

Reference

Alzyoud et al., 2021

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

γTub23C

bait

fused to GAL4 DNA-binding domain

Grip128

prey

fused to GAL4 activation domain

Grip128

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Grip128

C-terminal half

sufficient binding region

aa 551-975

coordinates relative to Grip128-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Alzyoud et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alzyoud et al., 2021)

FBrf0251596-18.PD.A

Reference

Alzyoud et al., 2021

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Grip128

bait

GST tag

Grip128

γTub23C

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Grip128

C-terminal half

sufficient binding region

aa 551-975

coordinates relative to Grip128-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Alzyoud et al., 2021)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

Grip128,gammaTub23C

References (4)

Report Sections

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