FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO2, mir-ban
FlyBase ID
FBig0000098790
Interaction Network
Interactions Browser links
AGO2 network
mir-ban network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0179363-5.coIP.NB
Reference
Okamura et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

TAP tag

mir-ban 

ban

prey
mir-ban 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Okamura et al., 2004)

AGO2 associates only with the mature form of ban miRNA, not the pre-miRNA.

(Okamura et al., 2004)

Mature ban miRNA coimmunoprecipitates with AGO1, AGO2 and Fmr1 in this assay. By contrast, the ban pre-miRNA coimmunoprecipitates only with AGO1.

(Okamura et al., 2004)
FBrf0191204-6.coIP.NB
Reference
Rehwinkel et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

HA tag

mir-ban 

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ban (mature miRNA and pre-miRNA) coimmunoprecipitates with AGO1, and to a lesser extent, with AGO2 as well.

(Rehwinkel et al., 2006)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rehwinkel et al., 2006)
FBrf0221629-2.FB.A
Reference
Hur et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO2 

DmAgo2

bait

MBP tag

AGO2 
mir-ban 

bantam

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO2 
C-terminal domain
sufficient binding region
aa 816-1214

coordinates relative to AGO2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized and labeled in vitro.

(Hur et al., 2013)
FBrf0224072-3.coIP.NB
Reference
Yang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

mir-ban 

ban

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Yang et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)