FB2025_01 , released February 20, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
orb2, LIMK1
FlyBase ID
FBig0000103967
Interaction Network
Interactions Browser links
orb2 network
LIMK1 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224133-17.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
LIMK1 

LimK

enzyme

MBP tag

LIMK1 

LimK

enzyme

MBP tag

orb2 

Orb2

enzyme target

His tag, P[32] label

orb2 

Orb2

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0224133-19.coIP.WB
Reference
White-Grindley et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait
LIMK1 

LimK

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(White-Grindley et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)