FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ci, Usp7
FlyBase ID
FBig0000103975
Interaction Network
Interactions Browser links
ci network
Usp7 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0229673-1.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp7 

Usp7

bait

FLAG tag

Usp7 
Usp7 
ci 

Ci

prey

Myc tag

ci 
ci 
ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
P-AxxS motifs S1-S4
sufficient binding region
aa 1-225

strongest binding, coordinates relative to ci-PA

ci 
P-AxxS motifs S5-S12
sufficient binding region
aa 441-900

moderate binding, coordinates relative to ci-PA

ci 
P-AxxS motifs S13-S16
sufficient binding region
aa 901-1160

moderate binding, coordinates relative to ci-PA

Usp7 
MATH domain
sufficient binding region
aa 90-220

coordinates relative to Usp7-PA

Usp7 
MATH domain
necessary binding region
aa 90-220

coordinates relative to Usp7-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)
FBrf0229673-2.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

Myc tag

ci 
ci 
Usp7 

Usp7

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-440

coordinates relative to ci-PA

ci 
central region
sufficient binding region
aa 440-1160

coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)
FBrf0229673-3.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait
Usp7 

Usp7

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The addition of hh-conditioned medium increased the ci-Usp7 interaction.

(Zhou et al., 2015)

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Zhou et al., 2015)
FBrf0229673-4.PD.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

GST tag

ci 
ci 
Usp7 

Usp7

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-440

coordinates relative to ci-PA

ci 
central region
sufficient binding region
aa 440-1160

coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Zhou et al., 2015)
FBrf0229673-5.PD.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp7 

Usp7

bait

GST tag

Usp7 
ci 

Ci

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp7 
N-terminal region
sufficient binding region
aa 1-234

coordinates relative to Usp7-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Zhou et al., 2015)
FBrf0241572-5.coIP.WB
Reference
Sun et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

HA tag

Usp7 

Usp7

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)