FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smo, Cul4
FlyBase ID
FBig0000116697
Interaction Network
Interactions Browser links
smo network
Cul4 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0239705-1.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
Cul4 

Cul4

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
SAID domain
sufficient binding region
aa 661-818
smo 
SAID domain
mutation decreasing interaction
aa 661-818

deletion of SAID domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment of cells with hh conditioned medium or a combination of Gβ13F and Gβ76C RNAi diminished the smo-Cul4 interaction.

(Li et al., 2018)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.

(Li et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)