Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.
