Isolation and characterization of Df(3L)BSC118
Rachel Andrade and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC118 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f05665 and P{XP}d01848. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; PBac{WH}f05665/P{XP}d01848 females crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC118 from the segment of PBac{WH}f05665 to the left of its FRT site and the segment of P{XP}d01848 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al.
Exelixis, Inc. determined the insertion site of PBac{WH}f05665 to be at Release 3 genomic coordinate 9473058 on chromosome arm 3L and the insertion site of P{XP}d01848 to be at Release 3 genomic coordinate 9654724 on arm 3L.
The Gene Disruption Project determined the insertion site of PBac{WH}f05665 to be at Release 3 genomic coordinate 9473201 on arm 3L and the insertion site of P{XP}d01848 to be at Release 3 genomic coordinate 9654720 on arm 3L. The cytological breakpoints of Df(3L)BSC118 predicted from these coordinates are 67B10;67C5. It failed to complement Df(3L)Exel6114, P{PZ}fry02240 and alphaTub67C1.