Isolation and characterization of Df(3R)BSC621
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC621 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CG5359e03976 and P{XP}Invadolysind08689. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{RB}CG5359e03976/P{XP}Invadolysind08689 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC621 from the segment of PBac{RB}CG5359e03976 to the left of its FRT site and the segment of P{XP}Invadolysind08689 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC621 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 85F5;85F14. Df(3R)BSC621 failed to complement knk1 and tws02414.