FB2025_01 , released February 20, 2025
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Citation
Bergner, L.M., Hickman, F.E., Wood, K.H., Wakeman, C.M., Stone, H.H., Campbell, T.J., Lightcap, S.B., Favors, S.M., Aldridge, A.C., Hales, K.G. (2010). A novel predicted bromodomain-related protein affects coordination between meiosis and spermiogenesis in Drosophila and is required for male meiotic cytokinesis.  DNA Cell Biol. 29(9): 487--498.
FlyBase ID
FBrf0211706
Publication Type
Research paper
Abstract
Temporal coordination of meiosis with spermatid morphogenesis is crucial for successful generation of mature sperm cells. We identified a recessive male sterile Drosophila melanogaster mutant, mitoshell, in which events of spermatid morphogenesis are initiated too early, before meiotic onset. Premature mitochondrial aggregation and fusion lead to an aberrant mitochondrial shell around premeiotic nuclei. Despite successful meiotic karyokinesis, improper mitochondrial localization in mitoshell testes is associated with defective astral central spindles and a lack of contractile rings, leading to meiotic cytokinesis failure. We mapped and cloned the mitoshell gene and found that it encodes a novel protein with a bromodomain-related region. It is conserved in some insect lineages. Bromodomains typically bind to histone acetyl-lysine residues and therefore are often associated with chromatin. The Mitoshell bromodomain-related region is predicted to have an alpha helical structure similar to that of bromodomains, but not all the crucial residues in the ligand-binding loops are conserved. We speculate that Mitoshell may participate in transcriptional regulation of spermatogenesis-specific genes, though perhaps with different ligand specificity compared to traditional bromodomains.
PubMed ID
PubMed Central ID
PMC2931543 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    DNA Cell Biol.
    Title
    DNA and Cell Biology
    Publication Year
    1990-
    ISBN/ISSN
    0198-0238
    Data From Reference
    Aberrations (4)
    Alleles (9)
    Genes (5)
    Natural transposons (1)
    Insertions (2)
    Transgenic Constructs (5)