FB2025_01 , released February 20, 2025
Sequence Feature: Dmel\dsRNA-KK115664
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General Information
Symbol
Dmel\dsRNA-KK115664
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000195640
Collection
Associated gene
Genomic Location
Chromosome (arm)
Sequence location
Genomic Maps

Multiple sequence regions exist. Please click on a sequence location for a JBrowse view of the region.

Sequence Data
Length
371
Comments

One copy of repeat shown.

(Keleman et al., 2009.8.5)

Sequence

Sequence Downloader
AGGAGTAGGAGAAGGAGCCGTTACTTCATCGTTTTGAACTGGTAAAACTTCCTCAACCACAACCGTGGAGCTGGATTCCT
CAATTGTTTCGGGAACAGAATCCACCTTGACCTCCTCCTGTTGCTTATCTTGTTCGGTGACAAATTCTGCTGCCTGTTCG
GTGATTTCAGCAACTATGCTGATTGCCTGCTGTTCGATCTCATCGGGCTTCACGTCGTTTACTTCGGGGACGTTCGGGGT
AACCTGCTCCTCGATAACTTCCTCGGAAAGCACAAATGTGGTGACAGGAGACGGAGGCGGGGATTTGGGCAATGGAGGTG
GCGGACTGCTGCTTCTCGTCTCCACAATAACGGACTCGAACTCAATTTCGG
Associated Information
Gene(s) (targeted or local)
    Allele(s)
      Transcripts(s)
        Polypeptide(s)
          Construct
          Experimental Data
          Progenitors
          PCR template

          genomic DNA

          (Keleman et al., 2009.8.5)
          Primer 1 
          AGGAGTAGGAGAAGGAGCCG
          (Keleman et al., 2009.8.5)
          Primer 2 
          CCGAAATTGAGTTCGAGTCC
          (Keleman et al., 2009.8.5)
          Comments
          Collection Information
          Collection: VDRC-KK
          Symbol
          VDRC-KK
          Title
          A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC second generation.
          Source and Progenitors
          Species of derivation
          D. melanogaster
          Strain of derivation
          Vector of progenitor construct
          pKC26
          Description

          Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.

          (Keleman et al., 2009.8.5)

          NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

          (FlyBase Curators, 2013)
          Additional data
          Sample preparation
          Collection preparation

          The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y+,w3'}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.

          (Keleman et al., 2009.8.5)

          The host strain used for transgene integration actually has two landing sites: the annotated site in the 5' UTR of tio at R6:2L:22 ,019,296 (P{attP,y+,w3'}VIE-260B), and a previously non-annotated site at R6:2L:9437482 (P{attP,y+,w3'}VIE-260B-2). The previously non-annotated site seems to be the main integration site, occupied in all 39 lines tested. The originally annotated site was occupied in only 9 of 39 lines tested.

          (Green et al., 2014)
          Mode of assay
          Data analysis
          Cell lines
          Observed in
          Not observed in
          Comments

          Targeted gene annotation (CG41555) was removed in subsequent FlyBase update.

          (Keleman et al., 2009.8.5)

          This sequence feature was not migrated from genomic release 5 to release 6: no unique site (within repeat).

          (Schroeder, 2014.6.12)
          Stocks (1)
          External Crossreferences and Linkouts ( 0 )
          Synonyms and Secondary IDs (2)
          Reported As
          Symbol Synonym
          115664
          (Keleman et al., 2009.8.5)
          dsRNA-KK115664
          Secondary FlyBase IDs
            References (2)