Dmel\P{XP}d08882 Insertion

General Information
Symbol	Dmel\P{XP}d08882	Species	D. melanogaster
Name		FlyBase ID	FBti0114964
Feature type	transposable_element_insertion_site
Description
Inserted element	P{XP}	Expression data
Affected gene(s)		Viability / fertility
Causes allele(s)		Stock availability	1 publicly available
LINE ID	d08882
Genomic Location
Chromosomal location	2L ( 35B8 )	Sequence location	2L:14,997,588..14,997,588 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{XP}.Exel.d A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004)
Sequence Location	2L:14,997,588..14,997,588 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004)
Cytological location (computed by FlyBase)	35B8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	35B8 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-, Parks et al., 2004)
Comments concerning location
Sequence Data
Flanking sequence	CZ476459 (Gene Disruption Project members, 2001-) CZ476460 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{XP}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.303Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Causes alleles

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	d08882 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- P{XP}d08882
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{XP}CG31834^d08882 P{XP}d08882 (FlyBase, 1992-, Thibault, 2004, Parks et al., 2004, ) P{XP}l(2)35Bb^d08882 (FlyBase, 1992-, Thibault, 2004)
Secondary FlyBase IDs
	FBti0056744 FBti0073548
References ( 11 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Parks et al., 2004, Nat. Genet. 36(3): 288--292 Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174230] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase Genome Annotators, 2006, Changes affecting gene model number or type in release 5.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 5.1 of the annotated D.melanogaster genome. [FBrf0191550] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]