- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
637bp deletion (coordinates X:8466141..8466777 , release 6 genome) that removes part of the CG33223 coding sequence. The deletion begins before the translation start site and completely blocks the synthesis of the CG33223 polypeptide.
A 637bp deletion that removes part of the dec2 coding sequence.
Eggs laid by Cp36dec2-1 mutant mothers are shorter and rounder than wild-type with a fragile chorion that is striped away when the egg is laid. However the follicle cells move outward over the nurse cells to generate tubes approximately wild-type in length. The chorion that is produced in mutant appendages appears disorganised and the dorsal appendages collapse when the eggs are laid.
The endochorion is completely disrupted in stage 14 mutant egg chambers, without notable effects on the innermost chorion layer. Eggs derived from homozygous females show significant uptake of neutral red (indicating defects in the vitelline membrane).
Thin chorion and short and thin respiratory appendages.
Homozygous females rarely oviposit. Eggs have thin chorion with thin respiratory appendages. female-sterile
Mohler.
The 'dec2[1]' mutant allele had previously been assumed to be a strong hypomorphic mutation of the Cp36 gene encoding the s36 protein. However sequencing shows that 1. the 'dec2[1]' mutant chromosome contains a deletion that removes part of the CG33223 annotation, and 2. cDNA sequences of the transcripts encoded by the Cp36 gene are identical in Oregon-R and dec2[1] mutant females and are identical to the annotated transcript of the release 6.56 Cp36 gene annotation in FlyBase. In addition, immunochemical analysis indicates that the s36 protein encoded by the Cp36 gene is produced in dec2[1] mutant females (and there is no significant difference in the level of protein produced compared to Oregon-R), but that while the s36 protein forms insoluble aggregates in protein lysates of eggs of Oregon-R females, in eggs laid by dec2[1] mutant females the s36 protein is in the soluble fraction and is not detected in the eggshell. Thus the dec2[1] mutation does not appear to directly affect the Cp36 gene, but instead appears to be a mutation of CG33223, which may be involved in regulating (either directly or indirectly) the secretion of the s36 protein from follicle cells into the eggshell in the wild-type.