ovoD3/+ females carrying ovoD1r+ produce embryos, but they die before hatching with gross and variable defects in cuticle differentiation including holes, especially anteriorly, segment deletions and fusions, failed head involution and deletions of anterior elements of the mouthparts.
Most of the egg chambers of heterozygous females develop into eggs that are laid (more than 30% of the wild-type number of eggs are laid per day). The eggs are usually permeable to neutral red and have fused dorsal appendages. The soma is wild-type. Transheterozygotes with either ovoM1, ovoM2 or ovoD1rv20 have germ cells resembling early spermatocytes.