Two tandem copies of a mutated 260bp twi proximal element (PE) (located between -440 and -180 relative to the twi promoter), in which i. the core sequences of the low affinity TD4 and TD5 dl binding sites have been mutated to the optimal sequence to create high affinity sites and ii. two copies of an E box from the rho promoter (with core sequence CATATG) have been inserted just upstream of the TD4 and TD5 sites, followed by twi sequence from -180 to +160bp relative to the transcription start site, are fused upstream of lacZ.
Expressed extremely broadly, over 28 to 30 cells in ventral and lateral region of the embryo. This domain includes the presumptive mesoderm, mesectoderm, and at least the ventral half of the neuroectoderm.
Expressed extremely broadly, over 28 to 30 cells in ventral and lateral region of the embryo. This domain includes the presumptive mesoderm, mesectoderm, and at least the ventral half of the neuroectoderm.
