FlyBase Allele Report: Dmel\Rac1[N17.hs]

General Information

Symbol

Dmel\Rac1^N17.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0042717

Feature type

allele

Associated gene

Dmel\Rac1

Associated Insertion(s)

Carried in Construct

P{N17DRacA}

Key Links

Transgenic product class

missense_variant

(Harden et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Harden et al., 1995)

Mutagen

in vitro construct

(Harden et al., 1995)

Progenitor genotype

Rac1^N17.UAS

(Harden et al., 1995)

Carried in construct

P{N17DRacA}

Cytology

Description

Expression of the mutated cDNA is driven by an Hsp70 promoter.

(Harden et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Rac1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell

(Murphy and Montell, 1996)

embryo | dorsal closure stage

(Harden et al., 1999)

embryonic/first instar larval cuticle

(Harden et al., 1999)

Detailed Description

Statement

Reference

Embryos expressing Rac1^N17.hs show a failure of dorsal closure. This phenotype is suppressed by Rac1^V12.Scer\UAS expressed under the control of Scer\GAL4^hs.PB.

(Harden et al., 1999)

Heat induced expression causes border cell migration to halted in 98% egg chambers. The actin cage that normally surrounds stage 11 nurse cell nuclei is absent resulting in ring canals becoming obstructed by the nuclei and failure to complete cytoplasm transfer.

(Murphy and Montell, 1996)

Heat shock at intervals from 4-5 hours to 9-10 AEL produces high frequency of defects in dorsal closure due to disruption of cell shape changes in the lateral epidermis, accompanied by disruption of a localised accumulation of actin and myosin thought to be driving epidermal cell shape change. Embryos also exhibit defects in germband retraction and head involution.

(Harden et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Cdc42^N17.UAS, Rac1^N17.hs, Scer\GAL4^hs.PB has embryonic/first instar larval cuticle | dorsal phenotype

(Harden et al., 1999)

Cdc42^V12.UAS, Rac1^N17.hs, Scer\GAL4^hs.PB has embryonic/first instar larval cuticle | dorsal phenotype

(Harden et al., 1999)

Cdc42^V12.UAS, Rac1^N17.hs, Scer\GAL4^hs.PB has embryonic/first instar larval cuticle phenotype

(Harden et al., 1999)

Cdc42^N17.UAS, Rac1^N17.hs, Scer\GAL4^hs.PB has embryonic/first instar larval cuticle phenotype

(Harden et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The dorsal closure defects of embryos co-expressing Cdc42^N17.Scer\UAS under the control of Scer\GAL4^hs.PB and Rac1^N17.hs (using heat shock) 4 to 8 hours after egg laying are more severe than the defects produced by expression of either Rac1^N17.hs or Cdc42^N17.Scer\UAS alone. In contrast, the defects produced by co-expression of Cdc42^N17.Scer\UAS under the control of Scer\GAL4^hs.PB and Rac1^N17.hs (using heat shock) 8 to 12 hours after egg laying are weaker than Rac1^N17.hs expressed alone, but stronger than Cdc42^N17.Scer\UAS expressed alone. When Rac1^N17.hs is co-expressed with Cdc42^V12.Scer\UAS under the control of Scer\GAL4^hs.PB most cuticles are extremely disrupted. There is evidence of some rescue of the Rac1^N17.hs phenotype by Cdc42^V12.Scer\UAS expressed under the control of Scer\GAL4^hs.PB, as there are greater numbers of wild-type and mild dorsal closure defective embryos than seen with the expression of Rac1^N17.hs alone.

(Harden et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Dominant inhibitory form of Rac1.

(Harden et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rac1^N17.hs

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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