Homozygous larvae raised at the restrictive temperature (37^oC for 6 hours/day from late embryogenesis through to third larval instar) show an increased frequency of ectopic neuromuscular synapses. This frequency is increased further if the larvae are also mutant for mle^nap-ts1 or para^ts1. Embryos raised at 18^oC or 34^oC show an increased frequency of immature filopodial contacts on muscle fibres 6 and 7 ("collateral sprouts") compared to wild-type embryos raised at the same temperature.

Flies become paralysed at 38-40^oC. The long-latency response of the dorsal longitudinal muscle disappears abruptly as the temperature is increased to 40 +/- 2^oC, although the short-latency response is normal. tipE^unspecified mle^nap-ts1 double mutant flies become paralysed at 31-32^oC. The long-latency response of the dorsal longitudinal muscle disappears abruptly as the temperature is increased to 31 +/- 2^oC, although the short-latency response is normal.