Embryos derived from germline clones of Df(1)G24 (which creates two mutations hep^G24 and lic^G24) or Df(1)G24; hep^Ubi-p63E.PG (hereafter referred to as lic^- embryos, as hep^Ubi-p63E.PG rescues the hep mutant phenotype) show novel and complex phenotypes, as compared to those generated using a simple hep mutation. 80-90% of the eggs appear not to be fertilised, are round in shape, and show a smaller size than the wild type. In addition the dorsal appendages are often (50%) shifted to a more dorsal position. In about 20% of the eggs the dorsal appendages are fused. In very rare cases (<1%) dorsalisation of the chorion is seen. lic^- mutant egg chambers show a highly penetrant "dumpless" phenotype, i.e. improper transfer of nurse cell content into the oocyte at the end of oogenesis. Cuticle preparations of developed lic^- embryos (10-20%) show variable but characteristic segmentation defects, consisting in the progressive deletion of abdominal segments (ranging from a partial deletion of segment A4 to a complete deletion of all abdominal segments), reminiscent of the posterior group genes. lic^- embryos lack or have strongly reduced numbers of pole cells. Expressing lic^Ubi-p63E.PS in these embryos rescues this phenotype.