Flies expressing Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^elav-C155 initially eclose in a rhythmic manner under constant darkness conditions.

However, approximately 2-3 days after transfer to constant darkness,

the flies appear to eclose in an arrhythmic fashion. Some rhythmicity

is seen in the eclosion profiles at 20^oC but none is seen at 25^oC.

Only 44.19% of male and 29.54% of female flies expressing Rmic\PDF^Scer\UAS.cHa

under the control of Scer\GAL4^elav-C155 show a clear rhythmicity

of locomotor activity when transferred to constant darkness conditions

(all controls are clearly rhythmic). Most flies are either complex

rhythmic or completely arrhythmic. Most of the complex rhythmic flies

show 2 simultaneously free-running components; one with a period of

approximately 22 hours and one with a period of approximately 25 hours.

Both free-running components seem to arise from the evening peak of

activity. The component with the longer period is generally more dominant

than that with the short period, and the flies with clear rhythmicity

show the long period component. The periods of both components are

highly significantly different from the periods of control flies.

Females show more extreme period alterations than male flies. The

flies show a significantly higher activity level under constant darkness

conditions than control flies.

Under 12 hour light:12 hour dark conditions, flies expressing Rmic\PDF^Scer\UAS.cHa

under the control of Scer\GAL4^elav-C155 extend their activity into

the dark period, in contrast to control flies where the activity is

more restricted to the light period. Both sexes show a highly significant

earlier morning peak and later evening peak than control flies.

Flies expressing Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^Mz1525

show a severely altered locomotor activity rhythm. The majority of

flies are either arrhythmic or show complex rhythmicity that is composed

of two activity components (one with a long period, one with a short

period, the short period component is more prominent), the activity

level of the flies under constant darkness conditions is increased,

and the phase of the morning peak is advanced. A phase delay is seen

in the evening peak only in female flies.

Flies expressing Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^Mz1366

show a severely altered locomotor activity rhythm. The majority of

flies are either arrhythmic or show complex rhythmicity that is composed

of two activity components of equal strength (one with a long period,

one with a short period), the activity level of the flies under constant

darkness conditions is increased, and the phase of the morning peak

is advanced. A phase delay is seen in the evening peak only in female

flies.

Flies expressing Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^Mz1172

show a normal rhythmic locomotory behaviour, although the period is

slightly shortened and the phase of the morning peak is slightly advanced

compared to controls.

No effect on general locomotor rhythmicity is seen in flies expressing

Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^P2.4.Pdf,

although the period is shorter than normal. The flies show lower activity

than normal under 12 hour light:12 hour dark conditions.

No effect on general locomotor rhythmicity is seen in flies expressing

Rmic\PDF^Scer\UAS.cHa under the control of Scer\GAL4^per.PK,

although the morning peak is slightly advanced compared to controls.

The flies show lower activity than normal under both constant darkness

and 12 hour light:12 hour dark conditions.