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General Information
Symbol
Scer\GAL4per.PK
Species
S. cerevisiae
Name
period promoter construct of Kaneko
FlyBase ID
FBal0060668
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
per-Gal4, period-Gal4, perGAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

4.2kb untranscribed sequence immediately upstream of the transcription start site to +25 site (25 bp into per mRNA) regulates expression of a GAL4 driver.

4.2kb genomic per fragment regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
s-LNv neuron

Comment: Variably labelled between samples

DN2 neuron

Comment: Variably labelled between samples

LNd neuron

Comment: Variably labelled between samples

periesophageal neuropils | restricted

Comment: Cells surrounding the esophageal foramen

eye

Comment: faint expression

ellipsoid body

Comment: strong expression

glial cell of adult brain

Comment: faint expression

Additional Information
Statement
Reference

Drives expression in larval and adult pacemaker neurons, including I-LNv and s-LNv neurons, a pair of dorsal anterior lateral neurons of the protocerebrum, and lateral neurons. Also drives expression in a number of midline neurons in late embryos.

Scer\GAL4per.PK drives expression in a subset of neurons in the ventral nerve cord - VGlut positive period-positive median segmental interneurons and a pair of more lateral neurons. Period-positive median segmental interneurons directly interact with Ecol\lexARapGAP1-OK6 expressing motor neurons as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

Scer\GAL4per.PK transcript is expressed in the RP2, aCC, pCC, U1 and U2 neurons. It is also often detected in the U3 and two EL neurons. Lineage analysis shows that Scer\GAL4per.PK is expressed in the UMI and VUM interneurons and in the interneurons that originate from neuroblasts MNB, NB2-1 and NB4-1.

Expression of Scer\GAL4per.PK is seen in cells located near sensilla on the head capsule, thorax and abdomen of the adult. Expression of Scer\GAL4per.PK in seen within the abdominal portion of the adult midgut. Expression in the mouth is restricted to the posterior region. Fibres from neurons expressing Scer\GAL4per.PK enter the distal most layer of the medulla. Expression of Scer\GAL4per.PK is seen in undefined cells located near to the lobula, and in a pair of cells on either side of the esophagus in the frontal cortex which project along posteriorly along the esophageal foramen. The neurons located dorsolateral to the antennal lobes can be subdivided into two groups: a larger one located dorsomedial to the smaller one. Neurons from both of these groups project to the central complex, with intense arborization in the ellipsoid body and the lateral triangle.

Putative per-expressing neurons in the adult brain: lateral neurons, dorsal neurons, Hofbauer-Buchner cells. Weak expression in some photoreceptors. Specific neurons in the brain in which endogenous PER has not been detected, such as neurons in central complex and pars intercerebralis.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Crei\ChR2H134R.UAS.mCherry with blue light) of Scer\GAL4per.PK cells in larvae results in paralysis, which does not occur in controls, for the duration of activation; local stimulation of a region of the ventral nerve cord results in local relaxation of the musculature in the associated larval segments and premature termination of peristalsis.

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4per.PK cells in larvae significantly decreases the speed of peristalsis and locomotion, compared to controls; the duration of segment contraction is significantly increased, compared to controls, with no effect on the extent of muscle contraction (muscle length); when inhibition is restricted to a smaller set of cells using Scer\GAL80tsh-GAL80, peristalsis occurs at a similar rate to controls; when inhibition is restricted to a smaller set of cells using Scer\GAL80R70C01, peristalsis is significantly faster than during inhibition of all Scer\GAL4per.PK cells.

Inhibition (using Npha\HRUAS.YFP with yellow light) of Scer\GAL4per.PK cells in larvae significantly decreases the speed of peristalsis, compared to controls; burst duration and pulses per burst of the transverse nerve are significantly increased, compared to control bursts; there is also an increased intersegmental delay in motor neuron bursting, compared to controls.

Inhibition (using VGlutHMS02011, in combination with a Dicer-2 transgene to enhance RNAi efficiency) of Scer\GAL4per.PK cells in larvae significantly decreases the speed of peristalsis, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
GAL4per.PK
Scer\GAL4per.PK
Name Synonyms
period promoter construct of Kaneko
Secondary FlyBase IDs
    References (40)