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General Information
Symbol
P{GAL4-per.BS}
FlyBase ID
FBtp0001321
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
per-Gal4, period-Gal4, perGAL4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Scer\GAL4per.PK
Product class / Tool use(s)
Description

4.2kb untranscribed sequence immediately upstream of the transcription start site to +25 site (25 bp into per mRNA) regulates expression of a GAL4 driver.

4.2kb genomic per fragment regulates expression of a GAL4 driver.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
gene
reporter
gene
Component Alleles
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
s-LNv neuron

Comment: Variably labelled between samples

DN2 neuron

Comment: Variably labelled between samples

LNd neuron

Comment: Variably labelled between samples

periesophageal neuropils | restricted

Comment: Cells surrounding the esophageal foramen

eye

Comment: faint expression

ellipsoid body

Comment: strong expression

glial cell of adult brain

Comment: faint expression

Additional Information
Statement
Reference

Drives expression in larval and adult pacemaker neurons, including I-LNv and s-LNv neurons, a pair of dorsal anterior lateral neurons of the protocerebrum, and lateral neurons. Also drives expression in a number of midline neurons in late embryos.

Scer\GAL4per.PK drives expression in a subset of neurons in the ventral nerve cord - VGlut positive period-positive median segmental interneurons and a pair of more lateral neurons. Period-positive median segmental interneurons directly interact with Ecol\lexARapGAP1-OK6 expressing motor neurons as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

Scer\GAL4per.PK transcript is expressed in the RP2, aCC, pCC, U1 and U2 neurons. It is also often detected in the U3 and two EL neurons. Lineage analysis shows that Scer\GAL4per.PK is expressed in the UMI and VUM interneurons and in the interneurons that originate from neuroblasts MNB, NB2-1 and NB4-1.

Expression of Scer\GAL4per.PK is seen in cells located near sensilla on the head capsule, thorax and abdomen of the adult. Expression of Scer\GAL4per.PK in seen within the abdominal portion of the adult midgut. Expression in the mouth is restricted to the posterior region. Fibres from neurons expressing Scer\GAL4per.PK enter the distal most layer of the medulla. Expression of Scer\GAL4per.PK is seen in undefined cells located near to the lobula, and in a pair of cells on either side of the esophagus in the frontal cortex which project along posteriorly along the esophageal foramen. The neurons located dorsolateral to the antennal lobes can be subdivided into two groups: a larger one located dorsomedial to the smaller one. Neurons from both of these groups project to the central complex, with intense arborization in the ellipsoid body and the lateral triangle.

Putative per-expressing neurons in the adult brain: lateral neurons, dorsal neurons, Hofbauer-Buchner cells. Weak expression in some photoreceptors. Specific neurons in the brain in which endogenous PER has not been detected, such as neurons in central complex and pars intercerebralis.

 
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

report comment: 4.2kb per insert.

Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (16)
References (40)