FlyBase Recombinant Construct Report: P{GAL4-per.BS}

General Information

Symbol

P{GAL4-per.BS}

FlyBase ID

FBtp0001321

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^per.PK

Also Known As

per-Gal4, period-Gal4, perGAL4, perG4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

8 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Scer\GAL4^per.PK

Transgenic product class

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Description

4.2kb untranscribed sequence immediately upstream of the transcription start site to +25 site (25 bp into per mRNA) regulates expression of a GAL4 driver.

(Kaneko, 1997.3.14)

4.2kb genomic per fragment regulates expression of a GAL4 driver.

(Plautz et al., 1997)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^per.PK

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

photoreceptor neuron

(Kaneko, 1997.3.14)

adult central complex

(Kaneko, 1997.3.14)

adult brain | restricted

(Kaneko, 1997.3.14)

adult pars intercerebralis

(Kaneko, 1997.3.14)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

larval RP2 motor neuron

(Ruiz et al., 2010)

larval DA1 motor neuron

(Ruiz et al., 2010)

pCC neuron

(Ruiz et al., 2010)

larval A1-7-innervating DO1 motor neuron

(Ruiz et al., 2010)

larval A1-7-innervating DA2 motor neuron

(Ruiz et al., 2010)

larval DA2 motor neuron

(Ruiz et al., 2010)

larval EL neuron

(Ruiz et al., 2010)

larval VUM neuron

Comment: interneuron

(Ruiz et al., 2010)

interneuron of neuroblast MNB

(Ruiz et al., 2010)

larval MP3 neuron

(Ruiz et al., 2010)

neuroblast NB2-1

Comment: 4 interneurons

(Ruiz et al., 2010)

neuroblast NB4-1

Comment: 2 interneurons

(Ruiz et al., 2010)

embryonic stage 17

larval H-cell sib

(Wheeler et al., 2006)

larval VUM interneuron

(Wheeler et al., 2006)

lineage MNB primary interneuron

(Wheeler et al., 2006)

first instar larval stage

GABAergic neuron | subset

(Babski et al., 2019)

wandering third instar larval stage

period-positive median segmental interneuron

(Kohsaka et al., 2014)

larval ventral nerve cord | restricted

(Kohsaka et al., 2014)

adult stage

antenna

(Kaneko and Hall, 2000)

adult antennal lobe | dorso-lateral | restricted

(Kaneko and Hall, 2000)

adult antennal lobe | restricted | ventro-lateral

(Kaneko and Hall, 2000)

proboscis

(Kaneko and Hall, 2000)

adult head capsule

(Kaneko and Hall, 2000)

leg

(Kaneko and Hall, 2000)

wing

(Kaneko and Hall, 2000)

adult thorax

(Kaneko and Hall, 2000)

adult abdomen

(Kaneko and Hall, 2000)

testis

(Kaneko and Hall, 2000)

seminal vesicle

(Kaneko and Hall, 2000)

male accessory gland

(Kaneko and Hall, 2000)

ejaculatory bulb

(Kaneko and Hall, 2000)

seminal receptacle

(Kaneko and Hall, 2000)

uterus

(Kaneko and Hall, 2000)

spermatheca

(Kaneko and Hall, 2000)

adult Malpighian tubule

(Kaneko and Hall, 2000)

adult midgut

(Kaneko and Hall, 2000)

pacemaker neuron

(Kaneko and Hall, 2000)

l-LNv neuron

(Liu et al., 2014, Kaneko and Hall, 2000)

adult s-LNv neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

DN1 neuron

(Kaneko and Hall, 2000)

DN2 neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

DN3 neuron

(Kaneko and Hall, 2000)

LNd neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

posterior optic commissure

(Kaneko and Hall, 2000)

outer medulla

(Kaneko and Hall, 2000)

ocellus | restricted

(Kaneko and Hall, 2000)

retina | restricted

(Kaneko and Hall, 2000)

photoreceptor | subset

(Kaneko and Hall, 2000)

eyelet photoreceptor cell

(Kaneko and Hall, 2000)

CNS surface glial cell | subset

(Kaneko and Hall, 2000)

glial cell of optic chiasma | subset

(Kaneko and Hall, 2000)

epithelial cell of adult heart | subset

(Kaneko and Hall, 2000)

follicle cell | subset

(Kaneko and Hall, 2000)

adult pars intercerebralis | restricted

(Kaneko and Hall, 2000)

cell body rind of the central nervous system | posterior | restricted

(Kaneko and Hall, 2000)

ellipsoid body | subset

(Kaneko and Hall, 2000)

fan-shaped body | subset

(Kaneko and Hall, 2000)

adult mediolateral antennal lobe tract | subset

(Kaneko and Hall, 2000)

adult periesophageal neuropils | restricted

Comment: Cells surrounding the esophageal foramen

(Kaneko and Hall, 2000)

lamina | restricted

(Kaneko and Hall, 2000)

somatic muscle cell of adult thorax | subset

(Kaneko and Hall, 2000)

adult protocerebrum | posterior | subset

(Kaneko and Hall, 2000)

neuron | subset of adult brain

(Chen et al., 2012)

neuron of the dorsal anterior lateral group of the protocerebrum

Comment: faint expression

(Chen et al., 2012)

eye

Comment: faint expression

(Chen et al., 2012)

ellipsoid body

Comment: strong expression

(Chen et al., 2012)

glial cell of adult brain

Comment: faint expression

(Chen et al., 2012)

antennal lobe projection neuron

Comment: medium expression

(Chen et al., 2012)

dorsal fusion commissure

(Kaneko and Hall, 2000)

Additional Information

Statement

Reference

Drives expression in larval and adult pacemaker neurons, including I-LNv and s-LNv neurons, a pair of dorsal anterior lateral neurons of the protocerebrum, and lateral neurons. Also drives expression in a number of midline neurons in late embryos.

(FlyBase Curators, 2017)

ScerGAL4^per.PK drives expression in a subset of neurons in the ventral nerve cord - VGlut1 positive period-positive median segmental interneurons and a pair of more lateral neurons. Period-positive median segmental interneurons directly interact with EcollexA^RapGAP1-OK6 expressing motor neurons as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

(Kohsaka et al., 2014)

ScerGAL4^per.PK transcript is expressed in the RP2, aCC, pCC, U1 and U2 neurons. It is also often detected in the U3 and two EL neurons. Lineage analysis shows that ScerGAL4^per.PK is expressed in the UMI and VUM interneurons and in the interneurons that originate from neuroblasts MNB, NB2-1 and NB4-1.

(Ruiz et al., 2010)

Expression of ScerGAL4^per.PK is seen in cells located near sensilla on the head capsule, thorax and abdomen of the adult. Expression of ScerGAL4^per.PK in seen within the abdominal portion of the adult midgut. Expression in the mouth is restricted to the posterior region. Fibres from neurons expressing ScerGAL4^per.PK enter the distal most layer of the medulla. Expression of ScerGAL4^per.PK is seen in undefined cells located near to the lobula, and in a pair of cells on either side of the esophagus in the frontal cortex which project along posteriorly along the esophageal foramen. The neurons located dorsolateral to the antennal lobes can be subdivided into two groups: a larger one located dorsomedial to the smaller one. Neurons from both of these groups project to the central complex, with intense arborization in the ellipsoid body and the lateral triangle.

(Kaneko and Hall, 2000)

Putative per-expressing neurons in the adult brain: lateral neurons, dorsal neurons, Hofbauer-Buchner cells. Weak expression in some photoreceptors. Specific neurons in the brain in which endogenous PER has not been detected, such as neurons in central complex and pars intercerebralis.

(Kaneko, 1997.3.14)

Marker for

l-LNv neuron

(Liu et al., 2014)

Reflects expression of

per

(Liu et al., 2014, Chen et al., 2012, Ruiz et al., 2010, Wheeler et al., 2006, Kaneko and Hall, 2000, Kaneko, 1997.3.14)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{GAL4-per.BS}

(Kaneko, 1997.3.14)

descendant(s)

Comments

report comment: 4.2kb per insert.

(Kaneko, 1997.3.14)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

7127

y¹ w^*; P{GAL4-per.BS}3

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

P(GAL4-per.BS)

(Kaneko, 1997.3.14)

Per-GAL4

(Wilhelm et al., 2022, Das et al., 2013)

Per-Gal4

(Babski et al., 2019, He et al., 2019, Burgos et al., 2018, Lo et al., 2015, Johard et al., 2009)

Period-GAL4

(Odierna et al., 2024)

P{GAL4-per.BS}

(Hürkey et al., 2023, FlyBase, 1996-)

P{w^+mC=GAL4-per.BS}

(Grönke et al., 2009)

per

(Joiner et al., 2006)

per-1b-Gal4

(Liu et al., 2014)

per-GAL4

(Colonna Romano et al., 2024, Liu et al., 2024, Wang et al., 2023, Suzuki et al., 2022, Wang et al., 2022, Kijak and Pyza, 2017, Kohsaka et al., 2014, Ruiz et al., 2010, Ishikawa et al., 1999)

per-gal4

(Pereanu and Hartenstein, 2006, Wegener et al., 2004, Helfrich-Forster et al., 2000, Kaneko and Hall, 2000, Kaneko et al., 2000)

per_P(4.2)-Gal4

(Goda et al., 2014)

perG4

(Colonna Romano et al., 2024, Hermann-Luibl et al., 2014)

perGAL4

(Faragó et al., 2019, Beckwith et al., 2013)

perGal4

(Pereanu et al., 2010)

period-GAL4

(Spindler and Hartenstein, 2011)

period-Gal4

(Lapraz et al., 2023, Kohsaka et al., 2019, Ruiz et al., 2010)

Secondary FlyBase IDs

References (57)

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