When retinal is not added to the food, larvae expressing Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} via Scer\GAL4^RapGAP1-OK6 display no discernible reaction to photostimulation, as measured by the degree of immobilization. When retinal is added, light application induces reversible muscle paralysis, with the duration of immobilization scaling with light intensity.

MN30-Ib neurons expressing Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^C380 depolarise and fire spikes when exposed to blue light.

Expression of Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^OK371 generates excitatory junctional potentials (EJPs) at the larval neuromuscular junction in response to 200ms blue light pulses.

Larvae expressing Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^OK371 under constant blue light illumination show significantly reduced crawling speeds from control larvae. The crawling speed of these larvae when exposed to pulsed blue light is not significantly different from that of controls.

Larvae expressing Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^5-40 and exposed to constant blue light are almost completely immobilised for the entire 2 minute stimulation period, in contrast to controls.

Larvae expressing Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^5-40 and exposed to rhythmic blue light are completely immobilised for the entire 2 minute stimulation period, in contrast to controls.