Open Close
General Information
Symbol
Dmel\hidUAS.cUa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Unknown
FlyBase ID
FBal0249394
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hid, UAShid
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of hid.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inducing cell death in somatic clones of terminal tracheal cells by expressing hidUAS.cUa under the control of Scer\GAL4btl.PU results in blebbing of the cytoplasm, loss of gass-filling and branch loss in third instar larvae.

Expression of hidUAS.cUa under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

Expression of hidUAS.cUa under the control of Scer\GAL4elav.PU in the embryonic ventral nerve cord significantly increases the volume of apoptotic particles and significantly decreases the numbers of EVE, DAC and CUT expressing neurons, compared to controls.

Adults expressing WScer\UAS.cUa under the control of Scer\GAL4Tdc2.PC in which all OA neurons are eliminated show axonal projection defects in Gr32a-expressing neurons in the brain, including a severe reduction and disorganisation of Gr32a-expressing leg and labellum termini.

The NimC4 and drpr-positive glial cells in embryos expressing hidScer\UAS.cUa in neurons under the control of Scer\GAL4elav.PU are abnormal in shape compared to controls as a result of increased glial engulfment of apoptotic particles. No significant difference in glial cell number is observed.

In contrast to wild-type D. melanogaster control males, D. melanogaster males expressing WScer\UAS.cUa under the control of either Scer\GAL4Gr32a.3.776 or Scer\GAL4Gr33a.3.315 show courtship towards D. virilis females. Despite rejection by the D. virilis females, the extent and quality of courtship displayed by these males resembles that seen with conspecific D. melanogaster females. D. melanogaster males expressing WScer\UAS.cUa under the control of either Scer\GAL4Gr32a.3.776 or Scer\GAL4Gr33a.3.315 also avidly court D. simulans and D. yakuba females.

D. melanogaster males expressing WScer\UAS.cUa under the control of either Scer\GAL4Gr22c.PD, Scer\GAL4Gr28b.d.PT, Scer\GAL4Gr28b.e.4.245, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39a.d.1.7 or Scer\GAL4Gr64f.9.7 do not show courtship towards D. virilis females.

Under thermophase/cryophase (TC) conditions (flies entrained initially under 12 hour light/12 hour dark conditions for 4 days at 18[o]C, followed by 7 days of thermophase (18 hours at 29[o]C) and cryophase (6 hours at 18[o]C) cycles in constant darkness), flies expressing WScer\UAS.cUa under the control of Scer\GAL4cry.PU show two broad peaks of activity. When these animals are subsequently allowed to free run at 18[o]C in the dark, 41% show rhythmic locomotor activity.

Overexpression of WScer\UAS.cUa in the developing wing (under the control of Scer\GAL4vg.PU), results in most flies emerging with ablated wings.

When WScer\UAS.cUa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C only 69% of pupae eclose. Eye size is reduced compared to controls.

Expression of WScer\UAS.cUa in larval wing disc cells, under the control of Scer\GAL4ptc-559.1 leads to extensive apoptotic cell death but does not result in invasive cell migration.

The lymph glands of larvae expressing WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ stain with acridine orange, indicating cell death. TUNEL labelling indicates that the cell death is due to apoptosis.

Expression of WScer\UAS.cUa in all circadian clock neurons (Scer\GAL4tim.PE) has no effect on light avoidance.

Expression of WScer\UAS.cUa under the control Scer\GAL4c42 results in lethality during embryonic and first instar larval stages.

Expression of WScer\UAS.cUa under the control of Scer\GAL4GMR.PF results in eye degeneration.

Expression of WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ affects the level of plasmatocytes in third instar larvae, with these larvae possessing less than 2% of non-apoptotic plasmatocytes. A significant percentage of these larvae reach pharate adult stage and emerge as phenotypically normal adults. Such adults contain very few remaining plasmatocytes compared to controls. These mutants reach the pupal stage in the same time as wild-type controls and do not contain melanotic tunours or any other obvious phenotypes.

Scer\GAL4Hml.Δ, WScer\UAS.cUa flies show a clear increased susceptibility to infection by both Gram-positive and Gram-negative bacteria. Approximately 67% of plasmatocyte-depleted Scer\GAL4Hml.Δ, WScer\UAS.cUa flies succumb to infection by Enterococcus faecalis within 68 hours of infection. This lethality is correlated with a faster and higher bacterial growth in plasmatocyte depleted Scer\GAL4Hml.Δ, WScer\UAS.cUa flies compared to wild-type. Similar susceptibility is observed when the flies are infected with fungi.

Approximately 55% of Scer\GAL4Hml.Δ, WScer\UAS.cUa larvae die during post-embryonic development, more specifically, at the pupal stage. This pupal partial lethality is completely suppressed by antibiotic treatment.

Expression of WScer\UAS.cUa under the control of Scer\GAL4GMR.PU results in adults with small, rough eyes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

Females co-expressing rprUAS.cUa and hidUAS.cUa under the control of Scer\GAL4Tk.PS show the expected mating-induced increase in germline stem cells in the germarium, as compared to controls.

One copy of SRm160B103 slightly suppresses the apoptosis seen in the eye when hidScer\UAS.cUa is expressed under the control of Scer\GAL4GMR.PU.

Selective ablation of octopamine/tyramine neurons in the brains through co-expression of hidScer\UAS.cUa and rprScer\UAS.cUa under the control of Scer\GAL4Tdc2.PC and VNC-specific Scer\GAL80tsh-GAL80 does not lead to any deficit in appetitive olfactory learning when fructose is given as a reward. Larvae also exhibit robust appetitive memory when sorbitol is used as a reward, comparable to that of genetic controls. In contrast, the larvae fail to show significant learning when rewarded with arabinose; memory performance is significantly reduced compared with controls. Perception of the odors (amyl acetate and benzaldehyde) is unaffected, and larvae show normal attraction behavior toward arabinose and perform indistinguishably from controls.

When embryonic Ilp7 neurons are killed using WScer\UAS.cUa and rprScer\UAS.C (post-embryonic Ilp7 neurons are retained by limiting expression to the first and second instar larval stages using Scer\GAL80ts.αTub84B) female egg laying is normal.

When males express WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Ilp7.PC female egg laying is reduced on the first day after eclosion compared to wild type males, but subsequent egg laying is normal.

Females expressing WScer\UAS.cUa and rprScer\UAS.C throughout development under the control of Scer\GAL4Ilp7.PC have severely reduced egg laying. These females also have distended abdomens and eggs are always found jammed in the lateral oviduct. Of the small number of egg laid by these females, only 40% produce viable larvae.

Activity peaks during thermophase/cryophase cycles are virtually eliminated in TrpA11 flies expressing WScer\UAS.cUa under the control of Scer\GAL4cry.PU. When these flies are subsequently allowed to free run at a constant temperature (either 18[o]C or 29[o]C) in constant darkness, only 6% show rhythmic locomotor activity.

The degree of wing ablation observed in flies expressing WScer\UAS.cUa under the control of Scer\GAL4vg.PU is strongly reduced in a Cdk7S164A.T170A background.

The co-expression of hidUAS.cUa and rprUAS.cUa under the control of Scer\GAL4ppk.PG induces some apoptosis in third instar larval sensory neurons, as compared to controls.

Expression of both WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Rh5.PT abolishes light avoidance at the larval stage. However, expression under the control of Scer\GAL4Rh6.PD does not affect light avoidance.

Expression of both WScer\UAS.cUa and rprScer\UAS.C efficiently ablates all circadian clock neurons (Scer\GAL4tim.PE), leaving larvae with a seriously disrupted light avoidance phenotype. This phenotype is no different in LD or DD conditions.

Expression of both WScer\UAS.cUa and rprScer\UAS.C efficiently ablates the lateral neurons (Scer\GAL4P2.4.Pdf) but has no effect on light avoidance.

hid110 mutants expressing both hidScer\UAS.cUa and rprScer\UAS.cUa in class IV dendritic arborization neurons (under the control of Scer\GAL4ppk.1.9) show markedly decreased white-light-avoidance behavior compared to wild-type and hid110 (bolwig-organ-ablated) mutants.

Expression of both hidScer\UAS.cUa and rprScer\UAS.cUa in class IV dendritic arborization neurons (under the control of Scer\GAL4ppk.1.9) results in marked decrease in white-light-avoidance behavior compared to wild-type.

Co-expression of either α4GT1EP797 or α4GT1dsRNA.Scer\UAS.P\T has no effect on the adult eye phenotype caused by expression of WScer\UAS.cUa under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference

Expression of Zzzz\E4orf4Scer\UAS.cPa suppresses the semi-viability seen when WScer\UAS.cUa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. 95% of pupae eclose (compared to 69%). The reduction in eye size is also partially suppressed.

Larval wing disc cells expressing BacA\p35Scer\UAS.cHa and WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1 display invading cells exclusively in basal planes of the tissue; these cells have cleanly detached and migrate several cell diameters away from the posterior edge of the ptc domain. They display a robust, rounded morphology indicative of healthy cells.

The BacA\p35Scer\UAS.cHa-WScer\UAS.cUa invasive cell phenotype is completely suppressed in the presence of msn06946.

The BacA\p35Scer\UAS.cHa-WScer\UAS.cUa invasive cell phenotype is completely suppressed by co-expression of bskK53R.Scer\UAS.

Knockdown of Nc, through expression of NcNIG.8091R completely suppresses the invasive cell phenotype found upon co-expression of BacA\p35Scer\UAS.cHa-WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1.

Reducing Ice levels, through expression of IceNIG.7788R, suppresses the invasive cell phenotype found upon co-expression of BacA\p35Scer\UAS.cHa-WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1.

Co-expression of BacA\p35Scer\UAS.cUa suppresses the cell death seen in the lymph glands of larvae expressing WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ.

Expression of BacA\p35Scer\UAS.cHa rescues the pupal partial lethality found upon expression of WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ through blocking plasmatocyte apoptosis.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
WScer\UAS.cUa
hidScer\UAS.cUa
hidUAS.cUa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs
    References (65)