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General Information
Symbol
Scer\GAL4NPF.1
Species
S. cerevisiae
Name
FlyBase ID
FBal0152204
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
npf-gal4, dNPF-Gal4, npfGal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 1kb fragment containing the NPF 5' regulatory region and part of the first exon regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

GAT13b. Alleles or genotypes used :P{UAS-GFP.U}

Scer\GAL4NPF.1 drives expression in NPF neurons that potentially overlap with ple positive dopaminergic neurons in the dorsolateral, basolateral and ipsilateral dorsomedial parts of the embryonic/larval protocerebrum, and with Tdc2 positive tyraminergic/octopaminergic neurons in the embryonic/larval subesophageal ganglion.

Scer\GAL4NPF.1 drives expression in some of the clock neurons, including, in males, three LNd neurons, three or four l-LNv neurons (weaker signal) and the Pdf negative s-LNV neuron. In females, Scer\GAL4NPF.1 drives expression in one less LNd and l-LNv neurons on average than males. Labelling is also detected in other cells in the central and lateral brain, including in the fan-shaped body. The expression pattern is similar to that of NPF protein, except that varicosities and axon terminals are less strongly stained with Scer\GAL4NPF.1.

Scer\GAL4NPF.1 drives expression in neurons of the dorsal and lateral protocerebrum, fan-shaped body and ellipsoid body. The expression is more widespread than the one obtained with NPF, except that neurons in the subesophageal ganglion labelled by NPF are not labelled with Scer\GAL4NPF.1.

Expression of Scer\GAL4NPF.1 is seen in three cells of the LNd neuronal cluster.

Expression in early third instar larvae is observed in four protocerebral neuropeptide F-positive neurons.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4NPF.1 cells in adult males results in significantly increased courtship towards females and males, compared to controls; this is not the case when Scer\GAL4NPF.1 and Scer\GAL4GMR71G01 cells are simultaneously inhibited.

Inhibition (using shits.FRT.stop.UAS at 31[o]C) of the Scer\FLP1fru-expressing subset of Scer\GAL4NPF.1 cells in adult males significantly increases courtship towards females, compared to controls; inhibition (using shits.UAS.FRT.cDa at 31[o]C) of a different subset of Scer\GAL4NPF.1 cells (inhibition restricted using Scer\FLP1fru) in adult males does not affect courtship towards females, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP or Cbβ\DT-AI.UAS) of Scer\GAL4NPF.1 in adult males significantly increases courtship and eliminates aggressive lunging behavior towards other males, compared to controls

Activation (using Bhal\NaChBacUAS.cNa) of Scer\GAL4NPF.1 cells in adult males does not affect courtship towards males and significantly increases aggressive lunging towards males, compared to controls.

Acute thermogenetic activation (using TrpA1Scer\UAS.cUa at 29[o]C) of Scer\GAL4NPF.1 neurons results in a significant decrease of sleep amount (especially during the 12hr dark phases), compared to controls; during the night, these flies also display significant decreases in average sleep bout length and bout number. This short-sleep phenotype progressively declines with prolonged activation, particularly during the light phase. Once back at 23[o]C, sleep reverts to normal levels in these flies. Thermogenetic activation (using TrpA1Scer\UAS.cUa at 29[o]C) of Scer\GAL4NPF.1 neurons in a NPFSK2/NPFSK2 background does not significantly affect sleep compared to controls (and these flies sleep significantly more than Scer\GAL4NPF.1>TrpA1Scer\UAS.cUa flies at 29[o]C). Presence of Scer\GAL80nSyb.PS reverts the significant decrease in sleep amount caused by acute thermogenetic activation of Scer\GAL4NPF.1 neurons. Presence of Scer\GAL80cry.PS significantly reverts the decrease in sleep amount caused by acute thermogenetic activation of Scer\GAL4NPF.1 neurons; total sleep is still significantly less than that seen in controls. Silencing Scer\GAL4NPF.1 neurons (via Hsap\KCNJ2UAS.cUa.EGFP hyperpolarization) does not significantly alter total sleep amount compared to controls; adult-specific overexpression (achieved using Gal80ts) also fails to produce significant changes in sleep behavior.

Acute thermogenetic activation (using TrpA1Scer\UAS.cUa at 29[o]C) of Scer\GAL4NPF.1 neurons results in a significant increase of food consumption (tested in both CAFE and FLIC assays) and a significant decrease in whole-fly triacylglyceride levels, compared to controls; these flies also show increased sensitivity to starvation conditions (they have a shorter lifespan than controls). Presence of Scer\GAL80cry.PS does not significantly change the increase in food consumption (measured by the CAFE assay) caused by acute thermogenetic activation of Scer\GAL4NPF.1 neurons.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 617 nm (red) light) of Scer\GAL4NPF.1 neurons in adults results in a significant preference for being in the region of activation (illuminated region), compared to controls; the rate of return to the illuminated area is increased and locomotion speed is reduced, compared to controls, but flies are not sedated; preference is lost in males when a virgin female is present in the non-illuminated area.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 617 nm (red) light) of Scer\GAL4NPF.1 neurons in adults in combination with a paired odor, results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

Optogenetic activation (using Crei\ChR2UAS.cSa with blue light activation) of Scer\GAL4NPF.1 neurons during training alone, but not testing alone, significantly impairs the short-term olfactory memory of larvae trained with 2 M fructose, compared to controls; this impairment was still seen when larvae were given additional training sessions, but not after fewer training sessions. Activation during training does not affect short-term aversive memory following training with 1.5 M NaCl. There is no alteration in short-term preference for an odor paired to optogenetic activation in the absence of nutritional stimuli and no innate difference in odor or sugar preference compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 33[o]C) of Scer\GAL4NPF.1 neurons during training and testing, significantly impairs the short-term olfactory memory of larvae trained with 2 M fructose, compared to controls; there is no impact on food intake of either 2 M fructose or pure agarose. There is no innate difference in odor preference compared to controls.

Larvae in which single cell clones (generated using hs-FLP during embryonic stage) of Scer\GAL4NPF.1 neurons are thermogenetically activated (using TrpA1UAS.Tag:MYC at 33[o]C) during training and testing, have alterations in short-term olfactory memory when trained with fructose, compared to controls in which no cell is activated; activation of the NPF-DL1 or NPF-DL2 cells abolishes appetitive memory; activation of the NPF-SOG cell results in aversive, rather than appetitive memory.

Ablation of Scer\GAL4NPF.1-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503. Suppressing expression in the ventral nerve cord using Scer\GAL80tsh-GAL80 results in reduced sensitivity to (S,Z,Z)-CH503.

Inactivation of neural activity in Scer\GAL4NPF.1-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference

Scer\GAL4NPF.1, TrpA1UAS.cUa has abnormal sleep | heat sensitive phenotype, non-suppressible by NPFUAS.cWa, Scer\GAL4NPF.1

Suppressor of
NOT Suppressor of
Statement
Reference

NPFUAS.cWa, Scer\GAL4NPF.1 is a non-suppressor of abnormal sleep | heat sensitive phenotype of Scer\GAL4NPF.1, TrpA1UAS.cUa

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of NPFKK111846 reduces the decreases in sleep amount seen in Scer\GAL4NPF.1>TrpA1Scer\UAS.cUa flies at 29[o]C compared to controls; total sleep in flies with knockdown is still less than that seen in controls. Co-expression of NPFScer\UAS.cWa does not significantly alter the decreases in sleep amount seen in Scer\GAL4NPF.1>TrpA1Scer\UAS.cUa flies at 29[o]C; total sleep in flies with over-expression is less than that seen in controls.

Larvae with ablation (using hidUAS.cUa and rprUAS.C) of Scer\GAL4NPF.1 neurons do not form significant short-term olfactory memory following training with 0.02 M fructose, but do following training with 2 M, fructose; in neither condition is there a significant difference compared to controls, which form significant memory in both conditions; food intake on 2 M fructose, but not pure agarose, is significantly reduced compared to controls. There is no innate difference in sugar preference compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4NPF.1
Scer\GAL4npf.1
Name Synonyms
Secondary FlyBase IDs
    References (64)