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General Information
Symbol
Crei\ChR2UAS.cSa
Species
C. reinhardtii
Name
Saccharomyces cerevisiae UAS construct a of Schroll
FlyBase ID
FBal0196250
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ChR2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of ChR2.

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When retinal is not added to the food, larvae expressing Crei\ChR2Scer\UAS.cSa via Scer\GAL4RapGAP1-OK6 display no discernible reaction to photostimulation, as measured by the degree of immobilization. When retinal is added, light application induces reversible muscle paralysis, with the duration of immobilization scaling with light intensity.

Even high intensity light fails to immobilize adult flies expressing Crei\ChR2Scer\UAS.cSa via Scer\GAL4RapGAP1-OK6.

In larvae expressing Crei\ChR2Scer\UAS.cSa via Scer\GAL4VGlut-OK371, 470-nm light triggers EJPs but only after relatively long light pulses (16 ms); 617-nm light pulses do not trigger EJPs in these animals.

Flies expressing Crei\ChR2Scer\UAS.cSa (a blue light-gated ion channel channelrhodopsin) under the control of Scer\GAL4Gr5a.5.156 show an attraction response to blue light when placed in an optogenetic T-maze containing blue and yellow light-emitting diodes. This response is not altered upon removal of the antennae. Flies expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Gr21a.9.323 avoid blue light, and this response is impaired upon removal of the antennae.

Flies expressing Crei\ChR2Scer\UAS.cSa (a blue light-gated ion channel channelrhodopsin) in PN[[v]]-1 neurons (under the control of Scer\GAL4VT1606 or Scer\GAL4VT48643) show an avoidance response to blue light when placed in an optogenetic T-maze containing blue and yellow light-emitting diodes. A similar avoidance response is seen when Crei\ChR2Scer\UAS.cSa is expressed in PN[[v]]-2 neurons under the control of Scer\GAL4NP7243 and this response was not altered by removing the antennae.

Flies expressing Crei\ChR2Scer\UAS.cSa (a blue light-gated ion channel channelrhodopsin) in PN[[v]]-3 neurons under the control of Scer\GAL4VT12760 show an impaired avoidance response to 0.5% CO[[2]]. No effect is seen on the avoidance response to 2% CO[[2]].

Activation of Crei\ChR2Scer\UAS.cSa by spaced blue light stimulation in animals expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4futsch-C380 results in a 2.5-fold increase in the number of "ghost boutons" (axon terminal extensions with synaptic vesicles but lacking active zones and postsynaptic structures) per neuromuscular junction compared to controls.

Blue light stimulation of octopaminergic neurons expressing Crei\ChR2Scer\UAS.cSa, under the control of Scer\GAL4Tdc2.PC, results in a significant increase in locomotor speed in response to starvation.

Expression of Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 increases response index to blue light-induced stimulation, indicating overexpression larvae are now more attracted (or less repelled) by blue light. Application of white light also induces a repression of the negative phototactic behavior of transgenic larvae.

Expression of Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 does not lead to adaptation of ORNs to prolonged blue light stimulation nor prevent the animals from perceiving an additional odor stimulus.

Transgenic larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Or33b.PC, Scer\GAL4Or45a.PC, Scer\GAL4Or45b.PC, Scer\GAL4Or59a.PF, Scer\GAL4Or67b.PC, Scer\GAL4Or74a.PC, Scer\GAL4Or85c.PC, or Scer\GAL4Or94b.PC are less attracted to dark quadrants (in orientation assays) than wild-type larvae. These flies also show a stronger negative phototaxis effect with blue light rather than white light (this wasn't seen with Scer\GAL4Or33b.PC and Scer\GAL4Or45a.PC drivers).

Transgenic larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Or33b.PC, Scer\GAL4Or45a.PC, Scer\GAL4Or45b.PC, Scer\GAL4Or59a.PF, Scer\GAL4Or67b.PC, Scer\GAL4Or74a.PC, Scer\GAL4Or85c.PC, or Scer\GAL4Or94b.PC avoid octyl acetate, as in wild-type.

Expression of Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Or33b.PC or Scer\GAL4Or45a.PC results in an aversion response to blue light.

MN30-Ib neurons expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4C380 depolarise and fire spikes when exposed to blue light.

Expression of Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4OK371 generates excitatory junctional potentials (EJPs) at the larval neuromuscular junction in response to 200ms blue light pulses.

Larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4OK371 under constant blue light illumination do not show significantly different crawling speeds from control larvae. The crawling speed of these larvae when exposed to pulsed blue light is not significantly different from that of controls.

Larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL45-40 and exposed to constant blue light crawl significantly more slowly than controls immediately after the onset of illumination, but by 30 seconds after the onset of illumination, crawling speeds are not different from those of controls.

Larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL45-40 and exposed to rhythmic blue light show a significant inhibition of crawling for the entire 2 minute stimulation period, in contrast to controls.

When subjected to light stimulation, motorneurons of larvae expressing Crei\ChR2Scer\UAS.cSa by Scer\GAL4C380 potentiates mEJP (spontaneous excitatory potentials) frequency on average to the same level as nerve stimulation, lasting 2-3 hours, and induces structural synaptic changes including the formation of "ghost boutons" - boutons containing synaptic vesicles but lacking active zones and presynaptic structures.

Larvae expressing Crei\ChR2Scer\UAS.cSa panneuronally (driven by Scer\GAL4elav.PLu) or in motorneurons (driven by Scer\GAL4D42) show light-induced body contractions

at 0.64 mW/mm2, whereas larvae expressing Crei\ChR2Scer\UAS.cSa in dopaminergic neurons (driven by Scer\GAL4Tdc2.PC) do not. In larvae expressing Crei\ChR2Scer\UAS.cSa in motorneurons, driven by Scer\GAL4D42, short light pulses (100 ms) evoke single excitatory junction potentials (EJPs).

Blue light illumination but not red light illumination impairs chemotaxis toward 3-octanol if Crei\ChR2Scer\UAS.cSa is expressed in motorneurons, under the control of Scer\GAL4D42. This effect is fully reversible and is not seen when Crei\ChR2Scer\UAS.cSa is expressed in octopaminergic/tyraminergic neurons, under the control of Scer\GAL4Tdc2.PC, or in dopaminergic neurons, under the control of Scer\GAL4ple.PF.

Larvae expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Tdc2.PC show the same appetitive memory after pairing one of two odors (n-amylacetate and 3-ocatnol) with fructose seen in control larvae. When light stimulation is used instead of the fructose reward during training, Scer\GAL4Tdc2.PC>Crei\ChR2Scer\UAS.cSa larvae show appetitive memory while control larvae do not. Similarly, when light substitutes the NaCl reinforcer during training, Scer\GAL4ple.PF>Crei\ChR2Scer\UAS.cSa larvae show aversive memory while control larvae do not.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Flies expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4Tdc2.PC in the presence of all-trans retinal in a norpA1 background (to prevent any response to an optical stimulus) show a preference for blue light over yellow light in a choice assay.

Flies expressing Crei\ChR2Scer\UAS.cSa under the control of Scer\GAL4ple.PF in the presence of all-trans retinal in a norpA1 background show increased locomotor activity upon blue-light illumination. These flies show a significant preference for yellow light and aversion to blue light in a choice assay.

A TbhnM18 background eliminates the increase in locomotor speed seen upon blue light stimulation of octopaminergic neurons expressing Crei\ChR2Scer\UAS.cSa, under the control of Scer\GAL4Tdc2.PC.

Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Crei\ChR2Scer\UAS.cSa
Crei\ChR2UAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Schroll
Secondary FlyBase IDs
    References (33)