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General Information
Symbol
Scer\GAL4eve.RN2
Species
S. cerevisiae
Name
FlyBase ID
FBal0152207
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
RN2-Gal4, RN2Gal4, P{RN2-GAL4}, HGN1-Gal4, eve'-GAL4RN2, RN2-E-Gal4, eve-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Two tandem repeats of eve sequences from +7.9kb to +8.6kb (relative to the transcription start site) are fused upstream of eve promoter sequences from -275bp to +11bp followed by the GAL4 driver coding region. Sequence consisting of a 38bp multi-cloning sequence followed by eve DNA from +91 to +99 nucleotides and an ATG codon has been used to replace the GAL4 translation initiation signal with that of eve. eve 3' sequences from +1306bp to +1521bp are present downstream of the GAL4 coding region.

Two tandem repeats pof the RP2+a/pCC element from +7.9 (EcoRI) to +8.6 (NheI) is placed upstream of the eve 5' promoter. This controls expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
RP2 motor neuron

Comment: reference states 13 hours of developmental time

aCC neuron

Comment: reference states 13 hours of developmental time

dendrite of motor neuron

Comment: reference states 21 hours of developmental time

Additional Information
Statement
Reference

Drives expression in a subset of motor neurons at all stages.

Scer\GAL4eve.RN2 is expressed in a group of two to five neurons innervating the adult hindgut. These neurons innervate the circular muscles, and the epithelium underlying both the rectal valve and the inner muscle layers of the rectum.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

aCC neuron & dendrite, with Cdc42V12.UAS

RP2 neuron & cell body, with Cdc42V12.UAS

RP2 neuron & dendrite, with Cdc42V12.UAS

RP2 neuron & neurite, with Cdc42V12.UAS

Detailed Description
Statement
Reference

Optogenetic activation (using Crei\ChR2UAS.cSa with blue light activation) of Scer\GAL4eve.RN2 neurons in the larva results in excitatory junction potentials in anal sphincter muscles and strong contraction of anal sphincter muscles, but not other hindgut muscles, no excitation or contraction is seen due to blue light in controls.

Silencing (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4eve.RN2 neurons does not significantly alter the interval of anal sphincter opening, compared to controls; when Scer\GAL4P2.4.Pdf neurons neurons are additionally silenced, there is a significantly reduced frequency of defecation, compared to controls, with barely any hindgut movement.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (6)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4eve.RN2
Name Synonyms
Secondary FlyBase IDs
    References (44)