FlyBase Allele Report: Dmel\wit[B11]

General Information

Symbol

Dmel\wit^B11

Species

D. melanogaster

Name

FlyBase ID

FBal0096772

Feature type

allele

Associated gene

Dmel\wit

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,067,867..4,067,867 [-]

Nucleotide change:

C4067867T

Reported nucleotide change:

C684T

Amino acid change:

Q92term | wit-PA; Q92term | wit-PB

Reported amino acid change:

Q92term

(Marques et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1^Δ113M

(Kashima et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage (with wit^A12)

(Veverytsa and Allan, 2011)

abnormal neuroanatomy

(Bivik et al., 2015)

abnormal neuroanatomy (with wit^A12)

(James and Broihier, 2011, Nahm et al., 2010, Sweeney and Davis, 2002)

abnormal neuroanatomy | larval stage (with wit^A12)

(Kang et al., 2024, Chen et al., 2022, Liu et al., 2014, Kim and Marqués, 2012, O'Connor-Giles et al., 2008)

abnormal neuroanatomy | third instar larval stage (with wit^A12)

(Kim et al., 2019, Nahm et al., 2013, Haussmann et al., 2008)

abnormal neurophysiology (with wit^A12)

(van der Plas et al., 2006, Marques et al., 2003)

abnormal neurophysiology | larval stage (with wit^A12)

(Kim and Marqués, 2012)

lethal (with wit^A12)

(Banerjee et al., 2017, Marques et al., 2003)

lethal | recessive

(Harrison, 1999.5.12)

lethal - all die before end of P-stage (with wit^A12)

(Veverytsa and Allan, 2011)

some die during larval stage (with wit^A12)

(Veverytsa and Allan, 2011)

Phenotype Manifest In

apterous-expressing neuron of the lateral cluster of the thorax

(Bivik et al., 2015)

apterous-expressing neuron of the lateral cluster of the thorax Ap4

(Bivik et al., 2015)

axon (with wit^A12)

(Veverytsa and Allan, 2011)

bouton (with wit^A12)

(Collins et al., 2006, Sweeney and Davis, 2002)

embryonic/larval neuromuscular junction (with wit^A12)

(Kim and Marqués, 2012, Haussmann et al., 2008)

embryonic/larval neuromuscular junction | larval stage (with wit^A12)

(Kang et al., 2024)

embryonic/larval neuromuscular junction | third instar larval stage (with wit^A12)

(Kim et al., 2019, Khuong et al., 2010)

larval abdominal 2 neuron (with wit^A12)

(Liu et al., 2014)

larval abdominal 3 neuron (with wit^A12)

(Liu et al., 2014)

larval CCAP neuron (with wit^A12)

(Veverytsa and Allan, 2011)

muscle cell of A1-7 ventral longitudinal muscle 1 (with wit^A12)

(Veverytsa and Allan, 2011)

neuromuscular junction (with wit^A12)

(Liu et al., 2014, Nahm et al., 2010, Collins et al., 2006, Sweeney and Davis, 2002)

neuromuscular junction | third instar larval stage (with wit^A12)

(Banerjee et al., 2017, Cho et al., 2015)

NMJ bouton (with wit^A12)

(Liu et al., 2014, Higashi-Kovtun et al., 2010, Haussmann et al., 2008)

NMJ bouton | decreased number | larval stage (with wit^A12)

(Chen et al., 2022)

NMJ bouton | increased number | third instar larval stage (with wit^A12)

(Nahm et al., 2013)

NMJ bouton | larval stage (with wit^A12)

(O'Connor-Giles et al., 2008)

NMJ bouton | third instar larval stage (with wit^A12)

(Kim et al., 2019, Cho et al., 2015)

postsynaptic density | third instar larval stage (with wit^A12)

(Banerjee et al., 2017)

presynaptic active zone | third instar larval stage (with wit^A12)

(Banerjee et al., 2017)

pupa (with wit^A12)

(Veverytsa and Allan, 2011)

subsynaptic reticulum | third instar larval stage (with wit^A12)

(Banerjee et al., 2017)

synapse (with wit^A12)

(Nahm et al., 2010, Marques et al., 2003)

terminal bouton | third instar larval stage (with wit^A12)

(Banerjee et al., 2017)

type I bouton | third instar larval stage (with wit^A12)

(James and Broihier, 2011)

type III bouton | decreased number (with wit^A12)

(Veverytsa and Allan, 2011)

Detailed Description

Statement

Reference

wit^B11/wit^A12 (but not wit^B11/+) third instar larvae display synaptic undergrowth with fewer mature and satellite boutons at the NMJ than controls.

(Kim et al., 2019)

wit^B11/wit^A12 transheterozygotes do not reach adulthood and present significant changes in the third instar larval neuromuscular junction compared to controls: a decrease in the number, but not in the area, of synaptic boutons; a significant increase in the active zone density; a significant increase in the total postsynaptic density length; a significant increase in the ruffle density at the synapse; and significant decreases in the subsynaptic reticulum length, but not density.

(Banerjee et al., 2017)

Mutants show loss of expression of P{FMRFa-EGFP.Tv}, a marker for the six Ap4 neurons in the developing ventral nerve cord, and loss of expression of eya, which identifies the four Ap cluster neurons.

(Bivik et al., 2015)

wit^A12/wit^B11 mutant third instar larvae do display synaptic undergrowth (reduced number of boutons) at neuromuscular junctions compared to controls.

(Cho et al., 2015)

wit^A12/wit^B11 mutants show a significant reduction in bouton number at neuromuscular junction 4.

(Liu et al., 2014)

wit^A12/wit^B11 third instar larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

(Nahm et al., 2013)

wit^B11/wit^A12 larvae show a reduction in synaptic terminal size at the neuromuscular junction (NMJ) compared to wild type. The frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the NMJ are decreased compared to wild type.

(Kim and Marqués, 2012)

wit^A12/wit^B11 animals display a 46% reduction in bouton number at neuromuscular junction 4.

(James and Broihier, 2011)

wit^A12/wit^B11 transheterozygotes exhibit approximately half the number of synaptic boutons as found in wild-type terminals.

(Higashi-Kovtun et al., 2010)

wit^A12/wit^B11 third instar larvae display small neuromuscular junctions.

(Khuong et al., 2010)

wit^A12/wit^B11 larvae exhibit synaptic undergrowth.

(Nahm et al., 2010)

aCC/RP2 synaptic current amplitude in wit^A12/wit^B11 mutants does not differ from wild-type.

(Fradkin et al., 2008)

wit^A12/wit^B11 larvae show a decrease in the number of type 1b boutons at muscle 13 compared to wild type.

(Haussmann et al., 2008)

The average total bouton number at the neuromuscular junction is decreased in wit^A12/wit^B11 larvae compared to controls.

(O'Connor-Giles et al., 2008)

wit^A12/wit^B11 mutants exhibit a dramatic reduction in bouton number (64%), branching (80%), and synaptic area (64%).

(Collins et al., 2006)

wit^A12/wit^B11 trans-heterozygous neuromuscular junctions exhibit very low EJP amplitudes and low quantal content, but maintain wild-type level mEJP amplitudes.

(van der Plas et al., 2006)

Mutant stage 13-14 embryos contain the normal number of crystal cells per embryo.

(Milchanowski et al., 2004)

At 25^oC less than 0.5% of wit^A12/wit^B11 animals escape from the pupal case.

(Marques et al., 2003)

wit^A12/wit^B11 larvae show a significant decrease in bouton number at the neuromuscular junction.

(Sweeney and Davis, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Scer\GAL4^elav.PU/twit^UAS.cKa

(Kim and Marqués, 2012)

NOT Enhanced by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-enhanceable by Scer\GAL4^G14/Dys^RNAi.NH2.UAS

(van der Plas et al., 2006)

Suppressed by

Statement

Reference

wit^B11/wit^HA3 has abnormal neuroanatomy phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E}

(McCabe et al., 2004)

wit^B11/wit^A12 has lethal phenotype, suppressible | partially by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

NOT suppressed by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has lethal - all die before end of P-stage phenotype, non-suppressible by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has some die during larval stage phenotype, non-suppressible by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^G14/Dys^RNAi.NH2.UAS

(van der Plas et al., 2006)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-suppressible by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

Enhancer of

Statement

Reference

wit^B11 is an enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

NOT Enhancer of

Statement

Reference

wit^B11/wit^A12 is a non-enhancer of abnormal neuroanatomy | larval stage | recessive phenotype of Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 is a non-enhancer of abnormal neurophysiology phenotype of Dys^{RNAi.Dp186.UAS}, Scer\GAL4^eve.RN2

(Fradkin et al., 2008)

Suppressor of

Statement

Reference

wit^B11/wit^A12 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^elav.PU, Sec15^KK101708

(Kang et al., 2024)

wit^B11 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | dominant | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

wit^B11/wit^A12 is a suppressor of abnormal size | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy phenotype of spict^mut

(Wang et al., 2007)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

NOT Suppressor of

Statement

Reference

wit^B11/wit^A12 is a non-suppressor of abnormal neuroanatomy | recessive | larval stage phenotype of Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit[+] is a non-suppressor of abnormal neuroanatomy phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

wit^B11/wit^A12 is a non-suppressor of abnormal neurophysiology phenotype of Dys^{RNAi.Dp186.UAS}, Scer\GAL4^eve.RN2

(Fradkin et al., 2008)

Other

Statement

Reference

Megf8^HSC, wit^B11 has abnormal neuroanatomy | larval stage phenotype

(Chen et al., 2022)

myd[+]/myd^3PM71, wit^B11 has abnormal flight phenotype

(Sidisky et al., 2021)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype

(Kim et al., 2019)

mtgo^e02963, wit^B11/wit[+] has partially lethal phenotype

(Syed et al., 2019)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype

(Liu et al., 2014)

Scer\GAL4^CCAP.PP, sax^CA.UAS.cUa, tkv^CA.UAS.cUa, wit^B11/wit^A12 has lethal - all die before end of P-stage phenotype

(Veverytsa and Allan, 2011)

Scer\GAL4^CCAP.PP, sax^CA.UAS.cUa, tkv^CA.UAS.cUa, wit^B11/wit^A12 has majority die during larval stage phenotype

(Veverytsa and Allan, 2011)

CCAP^UAS.cVa, Mip^UAS.cVa, Pburs^UAS.cVa, Scer\GAL4^CCAP.PP, wit^B11/wit^A12 has decreased body size phenotype

(Veverytsa and Allan, 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

wit^B11/wit^A12 has embryonic/larval neuromuscular junction phenotype, enhanceable by Scer\GAL4^elav.PU/twit^UAS.cKa

(Kim and Marqués, 2012)

NOT Enhanced by

Statement

Reference

wit^B11/wit^A12 has NMJ bouton | larval stage | decreased number phenotype, non-enhanceable by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has presynaptic active zone | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has postsynaptic density | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has subsynaptic reticulum | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has presynaptic active zone | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has postsynaptic density | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has subsynaptic reticulum | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has synapse phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

Suppressed by

Statement

Reference

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/tkv^CA.UAS.cUa/sax^CA.UAS.cUa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/CCAP^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Df(3R)3450/WASp¹

(Khuong et al., 2010)

wit^B11/wit^HA3 has neuromuscular junction phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E}

(McCabe et al., 2004)

NOT suppressed by

Statement

Reference

wit^B11/wit^A12 has NMJ bouton | larval stage | decreased number phenotype, non-suppressible by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Nrx-1^UAS.cLa

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Nrx-1^UAS.cLa

(Banerjee et al., 2017)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

Enhancer of

Statement

Reference

wit^B11/wit^A12 is an enhancer of neuromuscular junction | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of terminal bouton | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of neuromuscular junction | third instar larval stage phenotype of Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of terminal bouton | third instar larval stage phenotype of Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of NMJ bouton | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

NOT Enhancer of

Statement

Reference

wit^B11/wit^A12 is a non-enhancer of NMJ bouton | larval stage | decreased number phenotype of Megf8^HSC

(Chen et al., 2022)

Suppressor of

Statement

Reference

wit^B11/wit^A12 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^elav.PU, Sec15^KK101708

(Kang et al., 2024)

wit^B11 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11 is a suppressor of NMJ bouton | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11 is a suppressor of NMJ bouton | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of neuromuscular junction | third instar larval stage phenotype of Nrx-1^UAS.cLa, Scer\GAL4^elav.PU

(Banerjee et al., 2017)

wit^B11/wit^A12 is a suppressor of terminal bouton | third instar larval stage phenotype of Nrx-1^UAS.cLa, Scer\GAL4^elav.PU

(Banerjee et al., 2017)

wit^B11/wit^A12 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^B11/wit^A12 is a suppressor of neuromuscular junction | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit^A12 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction phenotype of spict^mut

(Wang et al., 2007)

wit^B11/wit^A12 is a suppressor of bouton | increased number phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

wit^B11/wit^A12 is a suppressor of neuromuscular junction phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

NOT Suppressor of

Statement

Reference

wit^B11/wit^A12 is a non-suppressor of NMJ bouton | larval stage | decreased number phenotype of Megf8^HSC

(Chen et al., 2022)

wit^B11/wit[+] is a non-suppressor of eye disc phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

wit^B11/wit[+] is a non-suppressor of glial cell phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Other

Statement

Reference

Megf8^HSC, wit^B11 has NMJ bouton | larval stage | decreased number phenotype

(Chen et al., 2022)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has NMJ bouton phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has neuromuscular junction phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 2 neuron phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 3 neuron phenotype

(Liu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

myd^3PM71/+, wit^B11/+ double heterozygotes show a decrease in flight ability.

(Sidisky et al., 2021)

Expressing Rac1^N17.UAS under the control of Scer\GAL4^elav-C155 in a wit^A12 background results in significantly fewer satellite boutons, but a similar number of mature synaptic boutons as controls at the NMJ of third instar larvae. The number of both bouton types is significantly reduced when expression is driven in a wit^A12/wit^B11 background.

(Kim et al., 2019)

mtgo^e02963, wit^B11 double heterozygotes eclose at a lower than expected mendelian ratio from cross between single heterozygotes.

(Syed et al., 2019)

The third instar larval neuromuscular junction defects observed in wit^B11/wit^A12 transheterozygotes is not suppressed or enhanced by either Nrx-1²⁷³ or Nlg1^Δ46 homozygosity.

The third instar larval neuromuscular junctions of individuals that both express Nrx-1^Scer\UAS.cLa under the control of Scer\GAL4^elav.PU and are wit^B11/wit^A12 transheterozygous show a decrease in the number of synaptic boutons similar to what is observed in wit^B11/wit^A12 transheterozygotes, rather than the increase induced by the expression of Nrx-1^Scer\UAS.cLa.

(Banerjee et al., 2017)

Syx4⁷³/+;wit^B11/+ double heterozygotic third instar larvae do not show significant differences in bouton number at the neuromuscular junction compared to wild type or single heterozygotes.

(Harris et al., 2016)

wit^A12/wit^B11 fully suppresses the increase in mature boutons and branch number seen at the neuromuscular junction of Fmr1^Δ113M/+ third instar larvae (branch number is significantly higher in wit^A12/wit^B11 larvae than Fmr1^Δ113M/+;wit^A12/wit^B11 larvae).

(Kashima et al., 2016)

Introducing a single copy of wit^A12 into the cpx^SH1 mutant background also rescues the synaptic overgrowth but not the elevated minis, combination with wit^A12/wit^B11 reduces the number of NMJ boutons in cpx^SH1 mutants even further, below the control levels and is comparable to that observed in wit^A12/wit^B11 mutants alone.

(Cho et al., 2015)

wit^A12/wit^B11 Acsl⁰⁵⁸⁴⁷/Acsl^KO double mutants show a significant reduction in bouton number at neuromuscular junction 4, similar to wit^A12/wit^B11 mutants.

(Liu et al., 2014)

Larvae that are heterozygous for both Neto³⁶ and wit^B11 have a similar number of boutons to wild type.

(Sulkowski et al., 2014)

wit^A12 spartin¹/wit^B11 Df(3R)110 double mutant larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls, as occurs in wit^A12/wit^B11 single mutant larvae.

(Nahm et al., 2013)

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU slightly, but significantly, enhances the reduction in synaptic terminal size at the neuromuscular junction seen in wit^B11/wit^A12 larvae.

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU has no effect on the size of the evoked excitatory junctional potential (EJP) at the neuromuscular junction in wit^B11/wit^A12 larvae.

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU has no effect on miniature EJP (mEJP) amplitude at the neuromuscular junction in wit^B11/wit^A12 larvae, while mEJP frequency is slightly increased.

(Kim and Marqués, 2012)

wit^A12/wit^B11 suppresses the increase in average bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

(Rodal et al., 2011)

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is not suppressed if the animals are also heterozygous for wit^B11.

(Yuva-Aydemir et al., 2011)

WASp¹/Df(3R)3450 partially suppresses the small neuromuscular junction phenotype seen in wit^A12/wit^B11 mutants.

(Khuong et al., 2010)

Expression of Cip4^NIG.15015R or WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57) in wit^A12/wit^B11 larvae does not affect overall bouton number or satellite bouton formation.

A wit^A12 heterozygous background can completely reverse the increase in total bouton number and satellite bouton formation in larvae expressing either Cip4^NIG.15015R or WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57).

(Nahm et al., 2010)

Post-synaptic expression of Dys^{dsRNA.mub.Scer\UAS} under the control of Scer\GAL4^eve.RN2 in a wit^A12/wit^B11 background increases the synaptic current as in a wild-type background.

(Fradkin et al., 2008)

A wit^A12/wit^B11 transheterozygous background suppresses the synaptic overgrowth and increased bouton number phenotypes found in nwk¹ homozygous third instar larvae, instead the larvae exhibit reduced synaptic growth and fewer boutons than wild type controls.

(Rodal et al., 2008)

spict^mut neuromuscular junction overgrowth phenotypes are fully suppressed in wit^A12/wit^B11 mutants. The synaptic undergrowth phenotypes in larvae homozygous for spict^mut in a wit^A12/wit^B11 background are indistinguishable from that of wit^A12/wit^B11 mutants alone.

(Wang et al., 2007)

Expression of Dys^{dsRNA.NH2.Scer\UAS} postsynaptically (under the control of Scer\GAL4^G14) in wit^A12/wit^B11 trans-heterozygous neuromuscular junctions results in similar EJP amplitudes and quantal content as in wit^A12/wit^B11 mutants.

(van der Plas et al., 2006)

Co-expression of tkv::wit^{wit-EC.tkv-IC.Ubi-p63E} and tkv::wit^{tkv-EC.wit-IC.Ubi-p63E} rescues the neuromuscular junction size in wit^HA3/wit^B11 animals.

(McCabe et al., 2004)

At 25^oC ~80% of wit^A12/wit^B11, Fmrf^Scer\UAS.cMa (driven by Scer\GAL4^dimm-929) animals can partially escape the pupal case. At 28^oC about 30% of females fully eclose, and some inflate their wings. While these animals move fairly well they usually die within several days without producing progeny. No rescue is seen in the size of the synapse. Nor is the primary defect in synaptic transmission rescued.

(Marques et al., 2003)

spin¹⁰⁴⁰³/spin^k09905 animals which are also mutant for wit^A12/wit^B11 show a decrease in bouton number.

(Sweeney and Davis, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wit^B11/wit^A12 is rescued by Scer\GAL4^elav-C155/wit^UAS.EGFP

(Smith et al., 2012)

wit^B11/wit^A12 is rescued by Scer\GAL4^elav-C155/wit^UAS.CFP

(Smith et al., 2012)

Partially rescued by

wit^B11/wit^A12 is partially rescued by Scer\GAL4^CCAP.PP/wit^UAS.cMa

(Veverytsa and Allan, 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5174

bw¹; wit^B11 st¹/TM6B, Tb¹

Kyoto

108086

bw¹; wit^B11 st¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Wit^B11

(Sidisky et al., 2021)

l(3)64Aa^B11

(Featherstone et al., 2000, Harrison, 1999.5.12)

wit^B11

(Kang et al., 2024, Zhou et al., 2023, Chen et al., 2022, Park et al., 2022, Peterson et al., 2022, Vuilleumier et al., 2022, Berndt et al., 2020, Kamimura et al., 2019, Kim et al., 2019, Syed et al., 2019, Vuilleumier et al., 2019, Wang et al., 2019, Banerjee et al., 2017, Deshpande et al., 2016, Harris et al., 2016, Kashima et al., 2016, Sulkowski et al., 2016, Berndt et al., 2015, Bivik et al., 2015, Cho et al., 2015, Kang et al., 2014, Liu et al., 2014, Sulkowski et al., 2014, Nahm et al., 2013, Kim and Marqués, 2012, Smith et al., 2012, James and Broihier, 2011, Pilgram et al., 2011, Rodal et al., 2011, Veverytsa and Allan, 2011, Yuva-Aydemir et al., 2011, Higashi-Kovtun et al., 2010, Khuong et al., 2010, Nahm et al., 2010, Chang et al., 2008, Fradkin et al., 2008, Haussmann et al., 2008, Miguel-Aliaga et al., 2008, O'Connor-Giles et al., 2008, Rodal et al., 2008, Baumgardt et al., 2007, Herrero et al., 2007, Korolchuk et al., 2007, Wang et al., 2007, Collins et al., 2006, van der Plas et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (59)

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