FlyBase Allele Report: Dmel\Nrx-1[UAS.cLa]

General Information

Symbol

Dmel\Nrx-1^UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0241963

Feature type

allele

Associated gene

Dmel\Nrx-1

Associated Insertion(s)

Carried in Construct

P{UAS-Nrx-1.L}

Also Known As

UAS-dnrx

Key Links

Transgenic product class

cDNA

(Li et al., 2007)

wild_type

(Li et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Li et al., 2007)

wild_type

(Li et al., 2007)

Mutagen

in vitro construct

(Li et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Nrx-1.L}

Cytology

Description

UAS regulatory sequences drive expression of Nrx-1 full-length cDNA.

(Li et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nrx-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Li et al., 2007)

abnormal neuroanatomy, with Scer\GAL4^futsch-C380

(Li et al., 2007)

Phenotype Manifest In

bouton, with Scer\GAL4^elav-C155

(Li et al., 2007)

bouton, with Scer\GAL4^futsch-C380

(Li et al., 2007)

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Banerjee et al., 2017)

terminal bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Banerjee et al., 2017)

Detailed Description

Statement

Reference

The expression of Nrx-1^Scer\UAS.cLa under the control of Scer\GAL4^elav.PU leads to a significant increase in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

(Banerjee et al., 2017)

Expression of Nrx-1^Scer\UAS.cLa pan-neuronally using Scer\GAL4^elav-C155 or Scer\GAL4^C380 significantly enhances the formation of synaptic boutons compared to wild-type. Increasing Nrx-1 gene dosage in all neurons by one copy results in over a 30% increase in the number of synaptic boutons, while increasing it by two copies results in more than 40% enhancement.

Expression of either one or two copies of Nrx-1^Scer\UAS.cLa in muscle cells, under the control of Scer\GAL4^C57 does not result in any significant change in bouton number.

(Li et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Nrx-1^UAS.cLa, Scer\GAL4^elav.PU has neuromuscular junction | third instar larval stage phenotype, suppressible by wit^B11/wit^A12

(Banerjee et al., 2017)

Nrx-1^UAS.cLa, Scer\GAL4^elav.PU has terminal bouton | third instar larval stage phenotype, suppressible by wit^B11/wit^A12

(Banerjee et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Nrx-1^UAS.cLa is a non-suppressor of neuromuscular junction | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Scer\GAL4^elav.PU/Nrx-1^UAS.cLa is a non-suppressor of terminal bouton | third instar larval stage phenotype of wit^B11/wit^A12

(Banerjee et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The third instar larval neuromuscular junctions of individuals that both express Nrx-1^Scer\UAS.cLa under the control of Scer\GAL4^elav.PU and are wit^B11/wit^A12 transheterozygous show a decrease in the number of synaptic boutons similar to what is observed in wit^B11/wit^A12 transheterozygotes, rather than the increase induced by the expression of Nrx-1^Scer\UAS.cLa.

(Banerjee et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Nrx-1^UAS.cLa rescues Nrx-1²⁷³

(Banerjee et al., 2017)

Scer\GAL4^futsch-C380/Nrx-1^UAS.cLa rescues Nrx-1²⁷³/Df(3R)5C1

(Li et al., 2007)

Nrx-1^UAS.cLa/Scer\GAL4^T80 rescues Nrx-1²⁷³/Df(3R)5C1

(Li et al., 2007)

Comments

The expression of Nrx-1^Scer\UAS.cLa under the control of Scer\GAL4^elav.PU rescues the decreased number of synaptic boutons observed at the Nrx-1²⁷³ homozygous third instar larval neuromuscular junction.

(Banerjee et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nrx-1^Scer\UAS.cLa

Nrx-1^UAS.cLa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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