FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\twitUAS.cKa
Open Close
General Information
Symbol
Dmel\twitUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kim
FlyBase ID
FBal0283236
Feature type
allele
Associated gene
Dmel\twit
Associated Insertion(s)
Carried in Construct
P{UAS-twit.K}
Key Links
Transgenic product class
wild_type
(Kim and Marqués, 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kim and Marqués, 2012)
Mutagen
in vitro construct
(Kim and Marqués, 2012)
Progenitor genotype
Carried in construct
P{UAS-twit.K}
Cytology
Description

UAS regulatory sequences drive expression of twit.

(Kim and Marqués, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
twit
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU results in a very minor, but significant, decrease in synaptic terminal size at the larval neuromuscular junction.

      (Kim and Marqués, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU slightly, but significantly, enhances the reduction in synaptic terminal size at the neuromuscular junction seen in witB11/witA12 larvae.

      Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU has no effect on the size of the evoked excitatory junctional potential (EJP) at the neuromuscular junction in witB11/witA12 larvae.

      Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU has no effect on miniature EJP (mEJP) amplitude at the neuromuscular junction in witB11/witA12 larvae, while mEJP frequency is slightly increased.

      (Kim and Marqués, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav.PU/twitUAS.cKa rescues Df(2L)BSC105/twit160

      (Kim and Marqués, 2012)
      Fails to rescue

      Scer\GAL4Mhc.PW/twitUAS.cKa fails to rescue Df(2L)BSC105/twit160

      (Kim and Marqués, 2012)
      Comments

      The decrease in the frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the neuromuscular junction which is seen in twit160/Df(2L)BSC105 larvae is rescued by expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU, but not by expression under the control of Scer\GAL4Mhc.PW.

      (Kim and Marqués, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      twitScer\UAS.cKa
      twitUAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kim
      Secondary FlyBase IDs
        References (1)