FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\twit160
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General Information
Symbol
Dmel\twit160
Species
D. melanogaster
Name
FlyBase ID
FBal0283233
Feature type
allele
Associated gene
Dmel\twit
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kim and Marqués, 2012)
Mutagen
Delta2-3 transposase
(Kim and Marqués, 2012)
Progenitor genotype
P{GT1}BG01978
(Kim and Marqués, 2012)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a deletion of 1522bp (nucleotides 2L:20852545..20854067 , release 5 genome coordinates) 3' to the original insertion site. The deletion removes part of twit.

(Kim and Marqués, 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:20,852,545..20,854,067 [+]
Comment:

1523 bp deletion resulting from imprecise excision of P{GT1}BG01978, which removes the transcription start, first exon, and part of the first intron of twit as well as 43 bp of intergenic DNA. The published coordinates were claimed to be relative to R4 but they are actually R5 coordinates.

(Kim and Marqués, 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The size of the synaptic terminal at the neuromuscular junction (NMJ) is normal in twit160/Df(2L)BSC105 larvae.

      The frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the NMJ are decreased in twit160/Df(2L)BSC105 larvae compared to wild type. The amplitude of the evoked EJP and membrane resting potential are normal at the mutant NMJ. Quantal content is increased compared to wild type.

      (Kim and Marqués, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(2L)BSC105/twit160 is rescued by Scer\GAL4elav.PU/twitUAS.cKa

      (Kim and Marqués, 2012)
      Not rescued by

      Df(2L)BSC105/twit160 is not rescued by Scer\GAL4Mhc.PW/twitUAS.cKa

      (Kim and Marqués, 2012)
      Comments

      The decrease in the frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the neuromuscular junction which is seen in twit160/Df(2L)BSC105 larvae is rescued by expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU, but not by expression under the control of Scer\GAL4Mhc.PW.

      (Kim and Marqués, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      #160
      (Kim and Marqués, 2012)
      twit160
      (Vuilleumier et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)