FlyBase Allele Report: Dmel\Snx16[3A.UAS.GFP]

General Information

Symbol

Dmel\Snx16^3A.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0260797

Feature type

allele

Associated gene

Dmel\Snx16

Associated Insertion(s)

Carried in Construct

P{UAS-Snx16.3A.GFP}

Also Known As

UAS-Snx16^3A-GFP

Key Links

Transgenic product class

missense_variant

(Rodal et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rodal et al., 2011)

Mutagen

in vitro construct

(Rodal et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Snx16.3A.GFP}

Cytology

Description

UAS regulatory sequences drive expression of a mutated form of Snx16 (contains the amino acid replacements E347A, E350A and E351A in the coiled-coil domain) which is tagged with GFP.

(Rodal et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Snx16

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Rodal et al., 2011)

Phenotype Manifest In

multivesicular body, with Scer\GAL4^elav-C155

(Rodal et al., 2011)

NMJ bouton | larval stage | increased number, with Scer\GAL4^elav-C155

(Rodal et al., 2011)

Detailed Description

Statement

Reference

Expression of Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155 results in an increase in both overall bouton number and in satellite bouton number at the larval neuromuscular junction. Multivesicular bodies are frequently observed in the boutons in these animals.

(Rodal et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has abnormal neuroanatomy | larval stage phenotype, enhanceable by Scer\GAL4^elav-C155/tkv^{Q199D.UAS.Tag:HA}

(Rodal et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^B11/wit^A12

(Rodal et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/nwk^{UAS.mRFP(Unk)}

(Rodal et al., 2011)

NOT Suppressor of

Statement

Reference

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Other

Statement

Reference

Scer\GAL4^VGlut1.PD, Snx16^3A.UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | third instar larval stage phenotype

(Deshpande et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has NMJ bouton | larval stage | increased number phenotype, enhanceable by Scer\GAL4^elav-C155/tkv^{Q199D.UAS.Tag:HA}

(Rodal et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has NMJ bouton | increased number | larval stage phenotype, suppressible by wit^B11/wit^A12

(Rodal et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/nwk^{UAS.mRFP(Unk)}

(Rodal et al., 2011)

NOT Suppressor of

Statement

Reference

Snx16^3A.UAS.GFP/tkv^{Q199D.UAS.Tag:HA}, Scer\GAL4^VGlut1.PD is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Other

Statement

Reference

Scer\GAL4^VGlut1.PD, Snx16^3A.UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | third instar larval stage phenotype

(Deshpande et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larvae expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} and Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^VGlut.PD display increased number of boutons on neuromuscular junctions.

(Deshpande et al., 2016)

Co-expression of nwk^{Scer\UAS.T:Disc\RFP-mRFP} does not suppress the increase in satellite bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

wit^A12/wit^B11 suppresses the increase in average bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

Co-expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} dramatically enhances the increase in satellite bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

(Rodal et al., 2011)

Xenogenetic Interactions

Statement

Reference

The reduced number of boutons on neuromuscular junctions as well as crawling defects observed in third instar larvae expressing Hsap\TARDBP^Scer\UAS.cHa under the Scer\GAL4^VGlut.PD driver cannot be restored by either by simultaneous co-expression of Snx16^{3A.Scer\UAS.T:Avic\GFP} and tkv^{Q199D.Scer\UAS.T:Ivir\HA1}.

(Deshpande et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Snx16^{3A.Scer\UAS.T:Avic\GFP}

Snx16^3A.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close