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General Information
Symbol
Dmel\DysdsRNA.NH2.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216503
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DysN-RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a dsRNA that targets sequence specific to the large isoforms of Dys (the dsRNA consists of an inverted repeat of nucleotides 610-1532 of accession number GB:AF297644, with the repeats separated by a spacer consisting of mub intron sequence).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overall activity of flies expressing DysdsRNA.NH2.Scer\UAS under the control of Scer\GAL4Act.PU is reduced compared to controls.

Flies expressing DysdsRNA.NH2.Scer\UAS under the control of Scer\GAL4Act.PU show moderate indirect flight muscle degeneration.

Expression of DysdsRNA.NH2.Scer\UAS under the control of Scer\GAL4Act.PU results in mild indirect flight muscle degeneration. Posterior crossvein defects are also observed.

Expression of DysdsRNA.NH2.Scer\UAS under the control of any of the following: Scer\GAL4sr-md710, Scer\GAL4Mef2.PR or Scer\GAL4how-24B does not significantly increase muscle degeneration compared to controls either in the embryos or the third instar larvae; neither does it affect pupal lethality.

Expression of DysdsRNA.NH2.Scer\UAS in the muscle, under the control of either Scer\GAL4how-24B or Scer\GAL4G14 results in quantal content levels that are increased to levels similar to that seen in Dys mutants (i.e. approximately 70% higher than controls). Expression of DysdsRNA.NH2.Scer\UAS pre-synaptically, under the control of Scer\GAL4elav-C155 does not change quantal content levels compared to wild-type larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by nAChRα6EY13897/nAChRalpha6[+]

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by l(3)j5B6j5B6/l(3)j5B6[+]

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by fra4/fra[+]

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by sli2/sli[+]

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by robo12/robo1[+]

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by msk[+]/msk5

DysdsRNA.NH2.UAS, Scer\GAL4Act.PU has visible phenotype, suppressible by l(3)L4092[+]/l(3)L4092L4092

NOT suppressed by
Statement
Reference
NOT Enhancer of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

One copy of wunk10201 suppresses the reduction in activity levels seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

Expression of wunGD1640 suppresses the reduction in activity levels seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of wunEMS4 suppresses the reduction in activity levels seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

Expression of laceScer\UAS.T:Ivir\HA1 suppresses the reduction in activity levels seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of captE636 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of captE593 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of mblE27 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Camn339 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Rack1EE enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of dyscc03838 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of uif2B7 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of chifBG02820a does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of chifEY05746 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Lis-1G10.14 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Lis-1k11702 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Lis-1k13209 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Nrkk14301 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of FhosA055 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of FhosEY09842 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of nAChRα6EY13897 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of nAChRα6KG05852 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of CG7845EMS-Mod4 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Rack1EY00128 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Rack11.8 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of uif1 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of gcmKG01117 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of gcmrA87 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of vimark16722 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of vimar09 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Dmnk16109 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of POSHk15815 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of delKG10262 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of del3 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Fkbp1400734 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of grhIM does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of robo12 suppresses the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of SP2353MB00605 does not enhance the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Df(2L)spdj2 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(2L)ade3 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(2L)ED1473 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(2R)ED2098 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(2R)en-B enhances the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Df(2R)en-A enhances the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Df(2R)PC4 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(2R)P34 does not modify the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3L)ED4978 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED5612 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED5942 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED6025 does not modify the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED6069 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED6076 does not modify the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)ED6265 suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Df(3R)Tl-P does not modify the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Camk04213 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of mblE27 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU. The indirect flight muscle degeneration phenotype is enhanced.

One copy of l(2)k04222bk04222b strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of nAChRα6EY13897 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of v(2)k14204k14204 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of l(3)j5B6j5B6 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Sema-2aunspecified weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of fra4 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of l(2)k03003k03003 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of sli2 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of l(2)k02205k02205 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of robo12 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Sdc10608 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of l(2)k10317k10317 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of stan192 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of wgspd-1 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of grhs2140 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of DlEMS-Mod130 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of DlEMS-Mod140 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Dadj1E4 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of dppKG08191 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of msk5 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of aosΔ7 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of dsx-c73Aj10E8 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Nrkk14301 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of hipkBG00855 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of kisk13416 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of CG4496KG10365 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of l(2)k11120ak11120a weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of POSHk15815 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of vimark16722 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of uifEMS-Mod29 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of CG7845EMS-Mod4 moderately suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of l(3)L4092L4092 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Sema-1ak13702 moderately enhances the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of mskEMS-Mod90 strongly enhances the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of gcmKG01117 strongly enhances the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

Expression of DysdsRNA.NH2.Scer\UAS postsynaptically (under the control of Scer\GAL4G14) in witA12/witB11 trans-heterozygous neuromuscular junctions results in similar EJP amplitudes and quantal content as in witA12/witB11 mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DysdsRNA.NH2.Scer\UAS
DysdsRNA.NH2.UAS
detdsRNA.NH2.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)