FlyBase Allele Report: Dmel\mbl[E27]

General Information

Symbol

Dmel\mbl^E27

Species

D. melanogaster

Name

FlyBase ID

FBal0082451

Feature type

allele

Associated gene

Dmel\mbl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Begemann et al., 1997)

Progenitor genotype

mbl^k05507b

(Begemann et al., 1997)

Cytology

Description

Imprecise excision removing exons 1 and 2.

(Begemann et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

exacerbates muscular dystrophy

modeled by Dg³²³

(Kucherenko et al., 2011)

modeled by Dg^O86

(Kucherenko et al., 2011)

modeled by Dg^RNAi.UAS

(Kucherenko et al., 2011)

modeled by Dys^RNAi.NH2.UAS

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female (with mbl^chst)

(Juni and Yamamoto, 2009)

abnormal developmental rate | first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS.EGFP

(Pascual et al., 2010)

abnormal developmental rate | first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS

(Pascual et al., 2010)

abnormal locomotor behavior | dominant | temperature conditional

(Kucherenko et al., 2011)

lethal

(Lawlor et al., 2012)

lethal (with Df(2R)Exel6066)

(Kahsai et al., 2016)

lethal (with mbl^chst-exC3)

(Juni and Yamamoto, 2009)

lethal (with mbl^chst-exC45)

(Juni and Yamamoto, 2009)

lethal (with mbl^chst-exC110)

(Juni and Yamamoto, 2009)

lethal (with mbl^chst-exS31)

(Juni and Yamamoto, 2009)

lethal (with mbl^E127)

(Juni and Yamamoto, 2009)

lethal (with mbl^k05507b)

(Juni and Yamamoto, 2009)

lethal | recessive

(Begemann et al., 1997)

lethal - all die before end of first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS.EGFP

(Pascual et al., 2010)

lethal - all die before end of first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS

(Pascual et al., 2010)

lethal - all die during embryonic stage

(Pascual et al., 2010)

lethal - all die during embryonic stage (with mbl^E16)

(Vicente et al., 2007, Monferrer and Artero, 2006)

lethal - all die during embryonic stage | recessive

(Monferrer and Artero, 2006, Artero et al., 1998)

partially lethal - majority die (with mbl^chst)

(Juni and Yamamoto, 2009)

partially lethal - majority die (with mbl^E2563-12)

(Juni and Yamamoto, 2009)

partially lethal - majority die (with mbl^k07110)

(Juni and Yamamoto, 2009)

some die during embryonic stage

(Pascual et al., 2010)

some die during embryonic stage (with mbl^E16)

(Vicente et al., 2007, Monferrer and Artero, 2006)

some die during embryonic stage | recessive

(Monferrer and Artero, 2006, Artero et al., 1998)

some die during first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS.EGFP

(Pascual et al., 2010)

some die during first instar larval stage, with Scer\GAL4^da.G32, mbl^C.UAS

(Pascual et al., 2010)

viable (with mbl^k07103)

(Juni and Yamamoto, 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies homozygous for mbl^E27 are not viable.

(Lawlor et al., 2012)

mbl^E27/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

mbl^E27 flies expressing mbl^{C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 do not undergo larval molting and die as first instar larvae up to three days after hatching.

mbl^E27 flies expressing mbl^C.Scer\UAS under the control of Scer\GAL4^da.G32 do not undergo larval molting and die as first instar larvae up to three days after hatching.

(Pascual et al., 2010)

mbl^chst/mbl^E27 females show reduced sexual receptivity compared to controls.

(Juni and Yamamoto, 2009)

mbl^E27/+ mutant flies do not exhibit any obvious indirect flight muscle defects.

(Kucherenko et al., 2008)

mbl^k07103/mbl^E27 escapers have wing blisters, wing venation defects or unexpanded wings.

(Prokopenko et al., 2000)

Mutants die as stage 17 embryos or during hatching and show no obvious defects in the formation of somatic and visceral muscles. At the end of embryogenesis embryos are severely paralysed, only twitching movements can be observed and their abdominal segments are strongly contracted. syt staining reveals the neuromuscular junctions can assemble normally. The extracellular tendon matrix (TM) is severely reduced, the muscles are forced to compete for epidermal surface at the segment border. At the ultrastructural level the thick and thin filaments are less ordered and dense in muscles, I-bands are absent, Z-bands are absent and instead only spindle-like concentrations of dark thin fibers can be seen.

(Artero et al., 1998)

Mutant clones in the eye exhibit phenotypic defects in many mutant ommatidia, the rhabdomeres of up to 4 outer photoreceptors have a smaller diameter resembling the rhabdomeres of receptor cell R7. All photoreceptors are abnormally differentiated and have malformed rhabdomeres that frequently do not extend into basal regions of the retina.

(Begemann et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mbl^E16/mbl^E27 has lethal | embryonic stage phenotype, suppressible | partially by Hsap\MBNL1^UAS.cGCa/Scer\GAL4^da.G32

(Vicente et al., 2007, Monferrer and Artero, 2006)

mbl^E16/mbl^E27 has lethal | embryonic stage phenotype, suppressible | partially by Hsap\MBNL1^UAS.cGCa/Scer\GAL4^Mef2.PR

(Vicente et al., 2007)

Enhancer of

Statement

Reference

mbl^k07103/mbl^E27 is an enhancer of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

mbl[+]/mbl^E27 is an enhancer of partially lethal - majority die | pupal stage | female limited | heat sensitive phenotype of Scer\GAL4^103Y, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

mbl^E27 is an enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

NOT Enhancer of

Statement

Reference

mbl[+]/mbl^E27 is a non-enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Suppressor of

Statement

Reference

mbl[+]/mbl^E27 is a suppressor | partially of visible phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

mbl^k07103/mbl^E27 is a suppressor of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

mbl[+]/mbl^E27 is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

mbl[+]/mbl^E27 is a suppressor of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

mbl[+]/mbl^E27 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

mbl[+]/mbl^E27 is a suppressor of short lived phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^elav-C155

(Li et al., 2008)

mbl^E27 is a suppressor of visible phenotype of svp^2.sev

(Begemann et al., 1997)

NOT Suppressor of

Statement

Reference

mbl[+]/mbl^E27 is a non-suppressor of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Other

Statement

Reference

Df(3R)Exel6184/+, mbl^E27 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

mbl^E16/mbl^E27 has abdomen | embryonic stage phenotype, suppressible | partially by Hsap\MBNL1^UAS.cGCa/Scer\GAL4^da.G32

(Monferrer and Artero, 2006)

Enhancer of

Statement

Reference

mbl[+]/mbl^E27 is an enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

mbl[+]/mbl^E27 is an enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

mbl^k07103/mbl^E27 is an enhancer of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

NOT Enhancer of

Statement

Reference

mbl[+]/mbl^E27 is a non-enhancer of abdominal tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Suppressor of

Statement

Reference

mbl[+]/mbl^E27 is a suppressor | partially of abdominal tergite phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

mbl^k07103/mbl^E27 is a suppressor of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

mbl[+]/mbl^E27 is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

mbl[+]/mbl^E27 is a suppressor of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

mbl[+]/mbl^E27 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

mbl^E27 is a suppressor of phenotype of Jra^Asp.hs.sev

(Begemann et al., 1997)

mbl^E27 is a suppressor of eye photoreceptor cell phenotype of svp^2.sev

(Begemann et al., 1997)

NOT Suppressor of

Statement

Reference

mbl[+]/mbl^E27 is a non-suppressor of abdominal tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Other

Statement

Reference

Df(3R)Exel6184/+, mbl^E27 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, mbl^E27 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg^RNAi.UAS, Scer\GAL4^Tub.PU, mbl^E27 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg³²³, mbl^E27 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Df(3R)Exel6184/+, mbl^E27 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, mbl[+]/mbl^E27 has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Dys^RNAi.C.UAS, mbl[+]/mbl^E27 has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

mbl Dys double heterozygous flies (mbl^E27/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

mbl^E27 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of mbl^E27 enhances the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of mbl^E27 enhances the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

mbl^E27 Dg³²³ double heterozygous flies exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Combination of Df(3R)Exel6184 in heterozygous state with a single copy of mbl^E27 results in a significantly increased frequency of lamina plexus defects in the third instar larval brain, whereas the rhabdomere length in the adult eye remains unaffected in the double heterozygotes.

Combination of Dg^O86 in heterozygous state with a single copy of mbl^E27 does not significantly affect the frequency of lamina plexus defects in the third instar larvae but results in a significantly reduced rhabdomere length in the adults.

(Marrone et al., 2011)

One copy of mbl^E27 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU. The indirect flight muscle degeneration phenotype is enhanced.

One copy of mbl^E27 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of mbl^E27 suppresses the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} in the presence of heterozygous mbl^E27 using Scer\GAL4^da.G32 leads to no significant change in tergite-phenotype proportion compared with expression of Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} alone.

Statistical analysis reveals a significant decrease in the proportion of flies expressing Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} using Scer\GAL4^da.G32 that show any phenotype in the presence of heterozygous mbl^E27 compared with flies expressing Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} alone. However, no change is observed when comparing the proportion within the strongest two categories only, perhaps indicating that only those with the weakest phenotype are suppressed.

(Lawlor et al., 2012)

The eye defects caused by expression of Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^sev.PU are not significantly modified by mbl^E27/+, but are enhanced by mbl^k07103/mbl^E27.

(Garcia-Lopez et al., 2008)

One copy of mbl^E27 partially suppresses the shortened lifespan seen when Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} is expressed in the developing eye under the control of Scer\GAL4^elav-C155.

(Li et al., 2008)

The embryonic lethality of mbl^E16/mbl^E27 animals is partially rescued by expression of Hsap\MBNL^{Scer\UAS.cGCa} under the control of Scer\GAL4^da.G32; 78.9% of the embryos hatch.

The embryonic lethality of mbl^E16/mbl^E27 animals is only slightly rescued by expression of Hsap\MBNL^{Scer\UAS.cGCa} under the control of Scer\GAL4^Mef2.PR; 18.0% of the embryos hatch.

(Vicente et al., 2007)

78.14% of mbl^E16/mbl^E27 embryos expressing Hsap\MBNL^{Scer\UAS.cGCa} under the control of Scer\GAL4^da.G32 hatch into larvae (in contrast to 0% of mbl^E16/mbl^E27 embryos) and the hypercontracted abdomen phenotype is greatly reduced.

(Monferrer and Artero, 2006)

Complementation and Rescue Data

Fails to complement

mbl^chst-exC45

(Juni and Yamamoto, 2009)

mbl^chst-exS31

(Juni and Yamamoto, 2009)

mbl^chst-exC110

(Juni and Yamamoto, 2009)

mbl^chst-exC3

(Juni and Yamamoto, 2009)

mbl^chst

(Juni and Yamamoto, 2009)

mbl^E127

(Juni and Yamamoto, 2009)

mbl^k05507b

(Juni and Yamamoto, 2009)

mbl^k07103

(Prokopenko et al., 2000)

mbl^k07103

(Prokopenko and Bellen, 1998.3.11)

Rescued by

mbl^E27 is rescued by mbl^C.UAS.EGFP/Scer\GAL4^da.G32

(Pascual et al., 2010)

mbl^E27 is rescued by mbl^C.UAS

(Pascual et al., 2010)

Partially rescued by

mbl^E16/mbl^E27 is partially rescued by mbl^C.UAS/Scer\GAL4^da.G32

(Vicente et al., 2007)

mbl^E16/mbl^E27 is partially rescued by mbl^A.UAS/Scer\GAL4^da.G32

(Vicente et al., 2007)

mbl^E16/mbl^E27 is partially rescued by mbl^B.UAS/Scer\GAL4^da.G32

(Vicente et al., 2007)

mbl^E16/mbl^E27 is partially rescued by mbl^C.UAS/Scer\GAL4^Mef2.PR

(Vicente et al., 2007)

mbl^E16/mbl^E27 is partially rescued by mbl^C.UAS/Scer\GAL4^da.G32

(Monferrer and Artero, 2006)

Comments

Fails to complement the reduced female sexual receptivity phenotype of mbl^chst.

mbl^E27 fails to complement the lethality of mbl^k05507b, mbl^chst-exC3, mbl^E127, mbl^chst-exC45, mbl^chst-exC110 and mbl^chst-exS31.

(Juni and Yamamoto, 2009)

Escapers from non-complementation with mbl^k07103 have wing blisters, wing venation defects and, occasionally, unexpanded wings.

(Prokopenko and Bellen, 1998.3.11)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7318

y¹ w^*; P{neoFRT}43D mbl^E27/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mbl^E27

(Wang et al., 2018, Lawlor et al., 2012, Kucherenko et al., 2011, Marrone et al., 2011, Pascual et al., 2010, Juni and Yamamoto, 2009, Garcia-Lopez et al., 2008, Kucherenko et al., 2008, Li et al., 2008, Vicente-Crespo et al., 2008, Vicente et al., 2007, Monferrer and Artero, 2006)

mbl^e27

(Li and Sean Millard, 2019)

mm^E27

Name Synonyms

Secondary FlyBase IDs

References (19)

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