FlyBase Allele Report: Dmel\Jra[Asp.hs.sev]

General Information

Symbol

Dmel\Jra^Asp.hs.sev

Species

D. melanogaster

Name

FlyBase ID

FBal0048828

Feature type

allele

Associated gene

Dmel\Jra

Associated Insertion(s)

Carried in Construct

P{sE-jun^Asp}

Key Links

Transgenic product class

missense_variant

(Treier et al., 1995)

polypeptide_post_translational_processing_variant

(Treier et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Treier et al., 1995)

polypeptide_post_translational_processing_variant

(Treier et al., 1995)

Mutagen

in vitro construct

(Su et al., 1998, Treier et al., 1995)

Progenitor genotype

Carried in construct

P{sE-jun^Asp}

Cytology

Description

The sev enhancer drives expression of a mutated Jra coding sequence in which a number of phosphorylation sites have been mutated to phosphomimetic residues: Asp replaces Ser or Thr at positions 58, 62, 63, 91 and 93. This results in a constitutively active protein.

(Treier et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

Jra

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | dominant

(Weber et al., 2000)

visible

(Begemann et al., 1997)

visible | dominant

(Weber et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

A 30 minute heat shock several hours before the beginning of dorsal closure in embryos carrying Jra^Asp.hs.sev results in less than 10% of embryos having defects. Those that do have very small anterior holes in the cuticle.

(Stronach and Perrimon, 2002)

Flies have rough eyes with ommatidia that often contain extra R7 cells. Most ommatidia with the normal complement of photoreceptors are symmetrical R3/R3-type clusters and/or are mis-rotated. The phenotype resembles that of fz^Scer\UAS.cAa driven by Scer\GAL4^hs.2sev. The eye disc reveals early polarity defects in the irregular arrangement and rotation angles of the clusters. Mosaic analysis reveals that Jra^Asp.hs.sev induces the R3 fate, as does over-activation of the fz pathway.

(Weber et al., 2000)

Expression causes transformation of cone cell precursors to photoreceptor neurons.

(Begemann et al., 1997)

Adults exhibit additional R7 receptor cells and general disorder of the eye. Phenotype is enhanced by loss of a functional copy of Jra (Jra¹ or Jra^IA109).

(Kockel et al., 1997)

Eye surface is irregular and the ommatidia often contain ectopic, extra R7-like photoreceptors. The extra R7 cells differentiate from cone cell precursors. These R7 cells are functional as determined by behavioral tests in a sev¹⁴ background.

(Treier et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Jra^Asp.hs.sev has visible phenotype, suppressible by mbl²⁵⁶³

(Begemann et al., 1997)

NOT suppressed by

Statement

Reference

Jra^Asp.hs.sev has abnormal planar polarity phenotype, non-suppressible by pnt¹⁵¹⁶⁶

(Weber et al., 2000)

Jra^Asp.hs.sev has abnormal planar polarity phenotype, non-suppressible by pnt¹⁹⁰⁹⁹

(Weber et al., 2000)

Suppressor of

Statement

Reference

Jra^Asp.hs.sev is a suppressor of lethal | recessive | germline clone | rescuable maternal effect | embryonic stage phenotype of Shark¹

(Fernandez et al., 2000)

NOT Suppressor of

Statement

Reference

Jra^Asp.hs.sev/Jra[+] is a non-suppressor of lethal | male | germline clone phenotype of Dok^PG155

(Biswas et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Jra^Asp.hs.sev has phenotype, enhanceable by aop^yan-1

(Treier et al., 1995)

Suppressed by

Statement

Reference

Jra^Asp.hs.sev has photoreceptor cell R7 | increased number phenotype, suppressible by pnt¹⁵¹⁶⁶

(Weber et al., 2000)

Jra^Asp.hs.sev has photoreceptor cell R7 | increased number phenotype, suppressible by pnt¹⁹⁰⁹⁹

(Weber et al., 2000)

Jra^Asp.hs.sev has phenotype, suppressible by mbl^E127

(Begemann et al., 1997)

Jra^Asp.hs.sev has phenotype, suppressible by mbl^E27

(Begemann et al., 1997)

Jra^Asp.hs.sev has phenotype, suppressible by mbl^k05507b

(Begemann et al., 1997)

Jra^Asp.hs.sev has cone cell phenotype, suppressible by mbl²⁵⁶³

(Begemann et al., 1997)

Jra^Asp.hs.sev has phenotype, suppressible by phyl^unspecified

(Treier et al., 1995)

Jra^Asp.hs.sev has phenotype, suppressible by pnt^Δ88

(Treier et al., 1995)

Jra^Asp.hs.sev has phenotype, suppressible by pnt¹²³⁰

(Treier et al., 1995)

Jra^Asp.hs.sev has phenotype, suppressible by pnt¹²⁷⁷

(Treier et al., 1995)

NOT suppressed by

Statement

Reference

Jra^Asp.hs.sev has photoreceptor cell R4 phenotype, non-suppressible by pnt¹⁵¹⁶⁶

(Weber et al., 2000)

Jra^Asp.hs.sev has photoreceptor cell R4 phenotype, non-suppressible by pnt¹⁹⁰⁹⁹

(Weber et al., 2000)

Suppressor of

Statement

Reference

Jra^Asp.hs.sev/Jra[+] is a suppressor of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of Dok^PG155

(Biswas et al., 2006)

Jra^Asp.hs.sev is a suppressor of embryonic dorsal epidermis | germline clone | rescuable maternal effect phenotype of Cka⁰⁵⁸³⁶

(Chen et al., 2002)

Jra^Asp.hs.sev is a suppressor of embryo | dorsal closure stage phenotype of slpr⁹²¹

(Stronach and Perrimon, 2002)

Jra^Asp.hs.sev is a suppressor of embryonic/first instar larval cuticle phenotype of slpr⁹²¹

(Stronach and Perrimon, 2002)

Jra^Asp.hs.sev is a suppressor of embryo | germline clone | dorsal closure stage phenotype of Shark¹

(Fernandez et al., 2000)

Jra^Asp.hs.sev is a suppressor | partially of embryonic/first instar larval cuticle | dorsal phenotype of Btk^k05610/Btk^k00206, Src42A^myri

(Tateno et al., 2000)

Jra^Asp.hs.sev is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of msn¹⁰²

(Su et al., 1998)

Jra^Asp.hs.sev is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of msn¹⁷²

(Su et al., 1998)

Jra^Asp.hs.sev is a suppressor of embryonic epidermis | dorsal phenotype of bsk¹

(Hou et al., 1997)

Jra^Asp.hs.sev is a suppressor of embryonic epidermis | dorsal phenotype of hep¹

(Hou et al., 1997)

Jra^Asp.hs.sev is a suppressor of embryonic epidermis | dorsal phenotype of Rac1^N17.UAS, Scer\GAL4^unspecified

(Hou et al., 1997)

NOT Suppressor of

Statement

Reference

Jra^Asp.hs.sev is a non-suppressor of embryonic epidermis | dorsal phenotype of Dsor1^LH110

(Hou et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from Dok^PG155 germ-line clones that carry one copy of the Jra^Asp.hs.sev transgene exhibit a less severe dorsal closure effect, both in terms of expressivity and penetrance, than Dok^PG155/Y embryos. The transgene-carrying embryos die at late embryonic stage and exhibit small anterior holes in the cuticle.

(Biswas et al., 2006)

The dorsal open phenotype seen in homozygous Cka⁰⁵⁸³⁶ embryos derived from females carrying homozygous Cka⁰⁵⁸³⁶ germ-line clones is substantially rescued by expression of Jra^Asp.hs.sev using heat shock.

(Chen et al., 2002)

The severe dorsal open phenotype of slpr⁹²¹/Y embryos is significantly rescued by expression of Jra^Asp.hs.sev (using a 30 minute heat shock several hours before the beginning of dorsal closure).

(Stronach and Perrimon, 2002)

Completely rescues the dorsal closure defects of shark¹ embryos derived from shark¹ female germline clones when expressed using heat shock between 5 and 10 hours after egg laying.

(Fernandez et al., 2000)

The dorsal open phenotype seen in some Btk29A^k00206 Src42A^myri/Btk29A^k05610 Src42A^myri embryos is partially rescued by Jra^Asp.hs.sev.

(Tateno et al., 2000)

Reduction of pnt dosage suppresses the supernumerary photoreceptor phenotype but has no effect on the tissue polarity defect.

(Weber et al., 2000)

Partially rescues the dorsal open phenotype of msn¹⁰²/msn¹⁷² embryos when expressed using heat shock.

(Su et al., 1998)

Phenotype can be dominantly suppressed by mbl²⁵⁶³ and other mbl mutants, number of extra photoreceptors in reduced. The polarity defects associated with the mutation are not affected.

(Begemann et al., 1997)

Jra^Asp.hs.sev cannot rescue the eye phenotype of pnt mutants, though pnt mutants can rescue the eye phenotype of Jra^Asp.hs.sev. phyl mutants also strongly suppress the eye phenotype of Jra^Asp.hs.sev.

(Treier et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Jra^Asp.hs.sev

Jun^act

(Riesgo-Escovar and Hafen, 1997)

Name Synonyms

Secondary FlyBase IDs

References (14)

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