FlyBase Allele Report: Dmel\Dok[PG155]

General Information

Symbol

Dmel\Dok^PG155

Species

D. melanogaster

Name

FlyBase ID

FBal0131545

Feature type

allele

Associated gene

Dmel\Dok

Associated Insertion(s)

P{GawB}Dok^PG155

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Bourbon et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{GawB}Dok^PG155

Cytology

Description

P{GawB} insertion in the first intron of the Dok gene, ~387bp upstream of the second exon.

(Biswas et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | germline clone

(Biswas et al., 2006)

lethal | germline clone | male | rescuable maternal effect

(Biswas et al., 2006)

lethal | recessive

(Bourbon et al., 2002)

partially lethal - majority die | male

(Biswas et al., 2006)

Phenotype Manifest In

chaeta

(Biswas et al., 2006)

embryonic/first instar larval cuticle | dorsal | germline clone | rescuable maternal effect

(Biswas et al., 2006)

embryonic leading edge cell & filamentous actin | germ-line clone

(Biswas et al., 2006)

interommatidial bristle

(Biswas et al., 2006)

orbital bristle

(Biswas et al., 2006)

thorax

(Biswas et al., 2006)

wing

(Biswas et al., 2006)

Detailed Description

Statement

Reference

1-2% of Dok^PG155/Y males survive to adulthood. These escapers exhibit four main phenotypes: 1. 50% have shrivelled wings 2. 30% exhibit a split thorax with a loss of bristles in the midline region of the thorax 3. <10% show a unilateral or bilateral loss of orbital bristles in the head region 4. <10% have irregularly placed bristles in the eye. Approximately 50% of embryos derived from Dok^PG155 germ-line clones exhibit a severe dorsal open phenotype. The lateral epidermal cells of these mutants are rounded, instead of being elongated as in wild-type embryos. Despite initially stretching prior to the failure of dorsal closure, Dok^PG155 germ-line clones show decreased levels of actin in all cells and a loss of the actin cable and actin protrusions at the leading edge. The Dok^PG155 germ-line escapers that do not die as embryos are always female. Paternally-rescued embryos show no dorsal phenotype and both males and females survive to adulthood. Dok^PG155/Y embryos show normal levels of actin distribution. However, the actin cable in the leading edge cells exhibits minor kinking.

(Biswas et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dok^PG155 has lethal | male | germline clone phenotype, non-suppressible by Jra^Asp.hs.sev/Jra[+]

(Biswas et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, suppressible by Jra^Asp.hs.sev/Jra[+]

(Biswas et al., 2006)

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, suppressible by puc^E69/puc[+]

(Biswas et al., 2006)

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, suppressible by Shark^hs.PF

(Biswas et al., 2006)

NOT suppressed by

Statement

Reference

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-suppressible by Scer\GAL4^unspecified/Src42A^CA.UAS

(Biswas et al., 2006)

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-suppressible by Src42A^hs.PL

(Biswas et al., 2006)

Dok^PG155 has embryonic/first instar larval cuticle | dorsal | germline clone phenotype, non-suppressible by Shark^K698R.hs

(Biswas et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from Dok^PG155 germ-line clones that carry one copy of the Jra^Asp.hs.sev transgene exhibit a less severe dorsal closure effect, both in terms of expressivity and penetrance, than Dok^PG155/Y embryos. The transgene-carrying embryos die at late embryonic stage and exhibit small anterior holes in the cuticle. Only 20% of embryos derived from Dok^PG155 germ-line clones that are also heterozygous for puc^E69 show a dorsal closure phenotype, compared to 48% of Dok^PG155 germ-line clones without a puc mutation. The rescued embryos have small holes in the cuticle. Only 10% of embryos derived from Dok^PG155 germ-line clones that carry two copies of the shark^hs.PF transgene show a dorsal closure phenotype; these embryos exhibit small anterior holes in the cuticle. In contrast, expression of the shark^K698R.hs transgene fails to rescue Dok^PG155 germ-line clone embryos. Expression of either the Src42A^hs.PL transgene or the activated Src42A^CA.Scer\UAS transgene fails to rescue Dok^PG155 germ-line clone embryos.

(Biswas et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Re-mobilisation of the transposon causes the lethality phenotype to be lost.

(Bourbon et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG2079^PG155

(Bourbon et al., 2002)

Dok^PG155

l(1)G0331^PG155

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close