axon & dorsal cluster neuron
embryonic leading edge cell & actin filament
The mild cleft thorax phenotype produced by Scer\GAL4pnr-MD237-mediated expression of Rab30dsRNA.Scer\UAS (without Dcr-2Scer\UAS.cDa) is enhanced in a background of hep1 heterozygosity or hemizygosity.
Ectopic expression of bskK53R.Scer\UAS, under the regulatory control of Scer\GAL4Ddc.PL in hep1 mutants restores the morphology of dorsomedial dopaminergic neuron. Expression of parkScer\UAS.T:Hsap\MYC in the notum of the wing imaginal discs, under the regulation of Scer\GAL4ap-md544, in hep1/+ mutants enhances the thoracic cleft phenotype observed in parkScer\UAS.T:Hsap\MYC/Scer\GAL4ap-md544 mutants alone.
pucE69/+, hep1/Y mutant flies have a decreased mortality rate in response to paraquat compared to hep1/Y single mutants, but an increased mortality rate compared to pucE69/+ single mutants. pucE69/+ flies with a hep1/Y background have a less pronounced extension of lifespan than pucE69/+ single mutant flies.
The dorsal hole phenotype, loss of leading edge actin cable, and loss of adhesion between the amnioserosa and dorsal epidermis of hep1 mutant embryos are all partially suppressed by Scer\GAL469B with dshΔDEP+.Scer\UAS, wgScer\UAS.cGa, dshScer\UAS.cAa, or dshΔDIX.Scer\UAS (in decreasing order of strength of suppression). armS10.Scer\UAS.T:Hsap\MYC with Scer\GAL469B weakly suppresses the dorsal hole phenotype as well as suppressing the adhesion phenotype, but fails to rescue the formation of an actin cable. panScer\UAS.cWa with Scer\GAL469B fails to significantly suppress the dorsal hole phenotype.
The small rough eye of Traf1Scer\UAS.T:Ivir\HA1; Scer\GAL4GMR.PF animals is markedly suppressed in a hep1 heterozygous background. The rprGMR.PW eye phenotypes are significantly suppressed by heterozygosity for hep1.
The severity of the dorsal closure phenotype of male hep1 hemizygotes from hep1 homozygous mothers is enhanced by heterozygosity for chic221 : 65% of dead embryos show failure to close the four posterior segments, compared to 30% in chic+.
The lethality of embryos which are maternally and zygotically hep1 is rescued by one copy of pucE69. The lethality of pucE69 homozygotes is rescued if the flies are also homozygous or hemizygous for hep1. The rescued flies look remarkably normal, except some females have macrochaetae with a kink.
cnomis1/cno3 embryos have no apparent defects in dorsal closure. hep1 dominantly enhances the cnomis1/cno3 phenotype; hep1/+ ; cnomis1/cno3 embryos have a dorsal open phenotype. The dorsal thoracic cuticle of hep1 homozygous adults is severely disrupted in a pydtam homozygous background.