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General Information
Symbol
Dmel\panUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of van de Wetering
FlyBase ID
FBal0059980
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dTCF, UAS-pan, UASdTcf, UAS-Tcf
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full length pan.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of panScer\UAS.cWa under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

Expression of panScer\UAS.cWa under the control of Scer\GAL4Act88F.PD does not result in a mutant wing phenotype.

Expression of panScer\UAS.cWa under the control of Scer\GAL4arm.PS results in approximately 1% embryonic lethality. Expression under the control of Scer\GAL4e22c results in approximately 1.8% embryonic lethality. Expression under the control of Scer\GAL4dpp.blk1 results in approximately 36.7% pupal lethality.

When panScer\UAS.cWa is driven by Scer\GAL4sev.EP eyes appear to be normal other than a weak interommatidial bristle phenotype.

Expression of panScer\UAS.cWa under the control of Scer\GAL4vg.int2.1 results in defects in the posterior wing margin.

Expression of panScer\UAS.cWa under the control of Scer\GAL4sd-SG29.1 results in reduced wings with severe patterning defects including loss of wing margin.

Expression of panScer\UAS.cWa under the control of Scer\GAL4Bx-MS1096 results in a reduction in the size of the wing. Ectopic bristles and vein tissue are seen throughout the wing blade and the wing margin is consistently nicked.

Scer\GAL4e22c-mediated expression causes full or partial embryonic viability or embryonic lethality. Viable lines show slight narrowing of the abdominal denticle belts.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference

panUAS.cWa/Scer\GAL430A is a non-enhancer of wing phenotype of disco-rbae-1

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of panScer\UAS.cWa partially suppresses the gnarled wing phenotype caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD.

Expression of both SoxNScer\UAS.cOa and panScer\UAS.cWa under the control of Scer\GAL4arm.PS results in 87.6% embryonic lethality. Expression under the control of Scer\GAL4e22c results in approximately 99.6% embryonic lethality, while expression of both SoxNScer\UAS.cOa and panScer\UAS.cWa under the control of Scer\GAL4dpp.blk1 results in approximately 100% pupal lethality.

Overexpression of panScer\UAS.cWa enhances the repressive capacity of overexpressed SoxNScer\UAS.cOa. This is particularly apparent when both transgenes are expressed under the control of Scer\GAL4arm.PS. Under these conditions, the cuticle pattern is most disrupted, both ventrally and dorsally. Ectopic denticles appear with greater frequency and broader distribution across the ventral naked cuticle domain.

panScer\UAS.cWa with Scer\GAL469B fails to significantly suppress the dorsal hole phenotype of hep1 embryo cuticles.

Co-expression of armScer\UAS.cSa partially suppresses the wing defects caused by expression of panScer\UAS.cWa under the control of Scer\GAL4Bx-MS1096 : the reduction in wing size, ectopic wing bristle phenotype and wing venation patterns are partially rescued, but the nicking of the wing margin is not suppressed.

Co-expression of armΔC.Scer\UAS and panScer\UAS.cWa under the control of Scer\GAL4Bx-MS1096 results in a small wing with a highly disrupted venation pattern and many ectopic bristles.

Co-expression of groNterm.Scer\UAS in animals expressing panScer\UAS.cWa under the control of Scer\GAL4Bx-MS1096 results in recovery of wing margin close to the hinge, but the reduced size of the wing and ectopic bristle phenotypes are not rescued.

panScer\UAS.cWa, Scer\GAL4C96 flies show minor wing nicking. Does not suppress wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

Xenogenetic Interactions
Statement
Reference

Expression of panScer\UAS.cWa under the control of Scer\GAL4sev.EP partially suppresses the loss of rhabdomeres seen in Hsap\HTTGMR.Q120 flies.

Co-expression of panScer\UAS.cWa decreases the fraction of flies with rough eyes in animals expressing Zzzz\Poly-AlaA37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2 under the control of Scer\GAL4ey.PB.

Hsap\MAPTGMR.Ex.PJ and panScer\UAS.cWa (driven by Scer\GAL4hs.2sev) enhance each other leading to more roughened anterior part of the eye, with fusion, pitting, and collapse of ommatidia along with irregularity of bristles.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
panScer\UAS.cWa
panUAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of van de Wetering
Secondary FlyBase IDs
    References (18)