FlyBase Allele Report: Zzzz\Poly-Ala[A37.UAS.EGFP,Tag:NLS(SV40-largeT)]

General Information

Symbol

Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

Species

a. artificial

Name

FlyBase ID

FBal0276825

Feature type

allele

Associated gene

Zzzz\Poly-Ala

Associated Insertion(s)

Carried in Construct

P{UAS-A37-EGFP.NLS}

Also Known As

UAS-NLS-A37

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Berger et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-A37-EGFP.NLS}

Cytology

Description

UASt drives expression of 37 polyalanine repeats that are tagged with EGFP and a nuclear localization signal (Tag:NLS(SV40-largeT)).

(Berger et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\Poly-Ala

Tagged with

EGFP

green fluorescent protein

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Berger et al., 2006)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal gravitaxis, with Scer\GAL4^nrv2.PS

(Berger et al., 2006)

abnormal locomotor behavior | adult stage, with Scer\GAL4^nrv2.PS

(Berger et al., 2006)

abnormal size, with Scer\GAL4^ey.PB

(Berger et al., 2006)

abnormal size | adult stage, with Scer\GAL4^ey.PB

(Berger et al., 2006)

partially lethal - majority die, with Scer\GAL4^ey.PB

(Berger et al., 2006, Berger et al., 2006, Berger et al., 2005)

partially lethal - majority die, with Scer\GAL4^nrv2.PS

(Berger et al., 2006)

partially lethal - majority live, with Scer\GAL4^Mhc.PW

(Berger et al., 2006)

short lived, with Scer\GAL4^ey.PB

(Berger et al., 2006)

short lived, with Scer\GAL4^Mhc.PW

(Berger et al., 2006)

short lived, with Scer\GAL4^nrv2.PS

(Berger et al., 2006)

visible, with Scer\GAL4^ey.PB

(Berger et al., 2006, Berger et al., 2005)

visible | adult stage, with Scer\GAL4^ey.PB

(Berger et al., 2006)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PB

(Berger et al., 2006, Berger et al., 2006, Berger et al., 2005)

eye | adult stage, with Scer\GAL4^ey.PB

(Berger et al., 2006)

rhabdomere | adult stage, with Scer\GAL4^elav-C155

(Berger et al., 2006)

Detailed Description

Statement

Reference

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB causes toxicity resulting in survival to adulthood and eye abnormalities. Survival of the mutants is increased by rapamycin treatment. Rapamycin also decreases the toxicity of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} in the eye.

(Berger et al., 2006)

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of either Scer\GAL4^nrv2.PS, Scer\GAL4^Mhc.PW or Scer\GAL4^ey.PB results in a reduction in lifespan. No reduction in survival is seen when Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} is expressed under the control of Scer\GAL4^elav-C155 at 25[o]C.

Flies expressing Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^nrv2.PS exhibit an impaired climbing response compared to controls.

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB at 25[o]C leads to the formation of abnormal eyes characterized by smaller size, abnormal shape and rough surface. At 29[o]C some flies exhibited complete loss of eyes.

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^elav-C155 leads to rhabdomere loss, but not until 20 days post-eclosion.

The expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT} under the control of Scer\GAL4^nrv2.PS, Scer\GAL4^ey.PB or Scer\GAL4^Mhc.PW lead to partially lethality before adulthood. Adult survivors expressing Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT} under the control of Scer\GAL4^nrv2.PS present a small decrease in lifespan as compared to controls. Adult survivors expressing Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT} under the control of Scer\GAL4^ey.PB exhibit a rough and small eye phenotypes, as compared to controls.

(Berger et al., 2006)

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB results in a rough eye phenotype which can be partially rescued by Li[+] or by AR-A014418.

The semi-lethality caused by expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB can be partially rescued by AR-A014418.

(Berger et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has visible phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has abnormal size phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has short lived phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has visible phenotype, suppressible | partially by Scer\GAL4^ey.PB/pan^UAS.cWa

(Berger et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has eye phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Suppressor of

Statement

Reference

Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}/Scer\GAL4^elav-C155 is a suppressor of rhabdomere | adult stage phenotype of Hsap\HTT^GMR.Q120

(Berger et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^elav-C155 suppresses the loss of rhabdomeres seen when Hsap\HTT^GMR.Q120 is expressed. An increase in visible rhabdomeres is seen at 1, 2 and 4 days post-eclosion.

One copy of Hsf¹ enhances the eye morphology and lifespan defects seen when Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} is expressed under the control of Scer\GAL4^ey.PB.

(Berger et al., 2006)

Co-expression of pan^Scer\UAS.cWa decreases the fraction of flies with rough eyes in animals expressing Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB.

(Berger et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Avic\GFP^{A37.EGFP.Scer\UAS.T:Zzzz\Poly-Ala.T:SV40\nls2}

Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls-largeT}

Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2}

Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

Name Synonyms

Secondary FlyBase IDs

FBal0276692
FBal0265630

References (4)

Report Sections

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