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Citation
Thomas, C., Rousset, R., Noselli, S. (2009). JNK signalling influences intracellular trafficking during Drosophila morphogenesis through regulation of the novel target gene Rab30.  Dev. Biol. 331(2): 250--260.
FlyBase ID
FBrf0215283
Publication Type
Research paper
Abstract

JNK-mediated closure of the Drosophila dorsal epidermis during embryogenesis is a well-characterised model for morphogenesis. However, little is known about how JNK signalling modifies particular cellular behaviours such as intracellular transport. Here we demonstrate that the gene encoding the small GTPase Rab30 is a new JNK transcriptional target whose function is required during embryonic and adult morphogenesis including JNK-dependent dorsal closure, embryonic head involution and thorax closure. Using immuno-fluorescence and live imaging, we show that EGFP-Rab30 localises to trans-Golgi in addition to small unidentified vesicles, and moves in a microtubule-dependent, polarised dorso-ventral manner in the leading edge during dorsal closure. We propose that JNK activity upregulates genes involved in intracellular transport in order to provide an increased level of trafficking activity in cells undergoing complex morphogenetic arrangements such as dorsal closure.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Biol.
    Title
    Developmental Biology
    Publication Year
    1959-
    ISBN/ISSN
    0012-1606
    Data From Reference
    Alleles (43)
    Genes (20)
    Natural transposons (1)
    Insertions (6)
    Experimental Tools (4)
    Transgenic Constructs (40)