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General Information
Symbol
Dmel\Rab5Q88L.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215394
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Rab5CA, UAS-Rab5-CA, UAS-YFP-Rab5-Q88L
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q88L.

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab5 that is tagged at the N-terminal end with YFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  epilepsy
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Appl.G1a does not impair axonal transport in the segmental nerves in third instar larvae.

Expression of Rab5HMC03420 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in formation of abnormal large vesicular structures in the egg chambers.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Bx-MS1096 results in enlarged endosomes.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eg-Mz360, Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4eg-Mz360 results in an increase in vesicle size in commissural neurons.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP using Scer\GAL4CU1 induces formation of enlarged early endosomes.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP via Scer\GAL4en-e16E results only rarely in multiple wing hairs.

Excitatory junctional current amplitude and quantal content at the neuromuscular junction are increased compared to controls in third instar larvae expressing Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4n-syb.PS.

Embryos expressing Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Mef2.PR show muscle detachment defects. These defects are due to separation between the integrins at the end of the muscles and the extracellular matrix.

Expression via Scer\GAL4pnr-MD237 results in a cleft thorax and shortened scutellar macrochaetae.

Clones expressing Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP (under the control of Scer\GAL4Act5C.PI) do not show differences in the adherens junctions.

Epithelial defects are seen in the egg chambers of females expressing Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL455B; 14% show cell swelling.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
NOT suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of circulating hemocytes characteristic for OcrlΔ3 mutant third instar larvae is not suppressed by Scer\GAL4He.PZ-driven expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR57C10 partially rescues the bang sensitivity of parabss1/+ flies, but does not rescue the bang sensitivity of hemizygous parabss1 flies or eas2 mutants.

The axon elongation defect seen in Stripdogi DL1 olfactory projection neuron single-cell clones is partially suppressed if the cells are also expressing Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP (expression driven by Scer\GAL4GH146).

Co-expression of rushScer\UAS.T:Avic\GFP and Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP using Scer\GAL4CU1 results in striking changes in endosome morphology: the Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP-induced large endosomes change their shape and form clusters of smaller interconnected vesicles.

Co-expression of rushK48E.Scer\UAS.T:Avic\GFP and Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP using Scer\GAL4CU1 results in endosome clustering.

Co-expression of rushR176G.Scer\UAS.T:Avic\GFP has no effect on the vesicle shape changes induced by Scer\GAL4CU1-mediated expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

Co-expression of Hrs2xFYVE.Scer\UAS.T:Avic\GFP has no effect on the vesicle shape changes induced by Scer\GAL4CU1-mediated expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

The penetrance of the tracheal lumen defects (convoluted and dilated dorsal trunks) caused by expression of VhldsRNA.Scer\UAS under the control of Scer\GAL4btl.PS is partially suppressed by co-expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP (under the control of Scer\GAL4Act5C.PI) in Rho172O clones does not worsen the Rho172O adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype that is not found in Rho172O clones.

Expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP (under the control of Scer\GAL4Act5C.PI) in Rho172F clones does not worsen the Rho172F adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype taht is not found in Rho172F clones.

The follicle cell epithelial defects seen in females expressing awddsRNA.Scer\UAS under the control of Scer\GAL455B are rescued by co-expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

Xenogenetic Interactions
Statement
Reference

The presence of axonal blockages in the segmental nerves characteristic for third instar larvae expressing Hsap\APP695.Scer\UAS under the control of Scer\GAL4Appl.G1a is not rescued by co-expression of Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into S2R+ cells.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP
Rab5Q88L.UASp.YFP
Name Synonyms
Secondary FlyBase IDs
    References (27)