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General Information
Symbol
Dmel\Vps35E42
Species
D. melanogaster
Name
FlyBase ID
FBal0221801
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

642 bp deletion resulting from the imprecise excision of P{EPgy2}Vps35EY14200

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion resulting in a 642bp deletion that removes part of the Vps35 transcription unit.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
model of  brain cancer
is ameliorated by kuzDN.UAS
is ameliorated by DlD.UAS
is ameliorated by DlHMS01309
is ameliorated by HrsUAS.cLa
is ameliorated by NGD14477
is ameliorated by Rab5S43N.UAS
is ameliorated by Rab7Q67L.UAS
is ameliorated by Su(dx)UAS.cCa
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

Parkinson's disease-related phenotypes are observed in Vps35E42 park25 double heterozygotes. No Parkinson's disease-related phenotypes are observed in Vps35E42 Pink1B9 double heterozygotes.

Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Vps35E42 mutant larvae exhibit supernumerary neuroblasts in the brain, which form tumors; transplantation of tumor tissue into the abdomen of host flies can results in large tumors that often metastasize to distant organs; the supernumerary neuroblasts do not show apparent defects in either cell polarity or asymmetric cell division (as assessed by the localization of Mira, aPKC and Numb during metaphase). Vps35E42 mutant INPs/ectopic neuroblasts display dramatically enlarged Rab7-positive late endosomes/MVBs; there are no apparent defects in lysosomes (GFP-LAMP1-positive), recycling endosome (Rab11-positive), Golgi (GFP-Golgi-positive) or mitochondria (mito-GFP-positive).

Vps35MH20/Vps35E42 mutants show supernumerary boutons and a dramatic reduction in larval locomotion.

Flies expressing Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show similar climbing ability to controls.

Flies expressing Vps35Scer\UAS.D650N under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show climbing defects.

Flies expressing Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show similar climbing ability to controls.

Flies expressing Vps35Scer\UAS.R550W under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show climbing defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Vps35E42 park25 double heterozygotes show an age-dependent reduction in climbing ability. No phenotypes are observed in either heterozygote alone.

Vps35E42 park25 double heterozygotes show increased sensitivity to the oxidative stressor paraquat compared with either heterozygote alone, similar to that seen in park25 homozygotes.

Vps35E42 Pink1B9 double heterozygotes do not exhibit climbing defects.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\VPS35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 25[o] fails to suppress the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Hsap\VPS35Scer\UAS.cMa at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35Scer\UAS.cMa under the control of Scer\GAL4da.PU suppresses the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Hsap\VPS35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU at 25[o] fails to suppress the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Hsap\VPS35D620N.Scer\UAS at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU partially suppresses the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Hsap\VPS35R524W.Scer\UAS under the control of Scer\GAL4da.PU at 25[o] fails to suppress the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Hsap\VPS35R524W.Scer\UAS at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35R524W.Scer\UAS under the control of Scer\GAL4da.PU partially suppresses the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Hsap\VPS35R524W.Scer\UAS under the control of Scer\GAL4da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Hsap\VPS35L774M.Scer\UAS under the control of Scer\GAL4da.PU at 25[o] fails to suppress the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Hsap\VPS35L774M.Scer\UAS at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35L774M.Scer\UAS under the control of Scer\GAL4da.PU partially suppresses the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Hsap\VPS35L774M.Scer\UAS under the control of Scer\GAL4da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Complementation and Rescue Data
Comments

The neuroblast brain tumor phenotype of Vps35E42 larvae is fully or partially rescued by the Scer\GAL4GMR9D11-driven expression of Vps35UAS.Tag:FLAG, depending on the Scer\GAL4GMR9D11 insertion.

Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.cMa at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Vps35Scer\UAS.D650N under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.D650N at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35Scer\UAS.D650N under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Vps35Scer\UAS.D650N under the control of Scer\GAL4da.PU partially rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.L800M at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU partially rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

Expression of Vps35Scer\UAS.R550W under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.R550W at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35Scer\UAS.R550W under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

Expression of Vps35Scer\UAS.R550W under the control of Scer\GAL4da.PU partially rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

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Stocks (0)
Notes on Origin
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)