FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vps35UAS.cMa
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General Information
Symbol
Dmel\Vps35UAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Malik
FlyBase ID
FBal0316902
Feature type
allele
Associated gene
Dmel\Vps35
Associated Insertion(s)
Carried in Construct
PBac{UAS-Vps35.M}
Key Links
Transgenic product class
wild_type
(Malik et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Malik et al., 2015)
Mutagen
in vitro construct
(Malik et al., 2015)
Progenitor genotype
Carried in construct
PBac{UAS-Vps35.M}
Cytology
Description

UASt regulatory sequences drive expression of full length Vps35.

(Malik et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vps35
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease
      modeled by Vps35MH20, Vps35E42
      (Malik et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Lifespan and motor ability are unaffected when Vps35Scer\UAS.cMa is expressed under the control of Scer\GAL4da.PU at 25[o]C. Lifespan is moderately shortened compared with controls at 29[o]C.

      No overt eye phenotypes are observed when Vps35Scer\UAS.cMa is expressed under the control of Scer\GAL4GMR.PU.

      Flies expressing Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show similar climbing ability to controls.

      (Malik et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Scer\GAL4da.PU/Vps35UAS.cMa is an enhancer of abnormal flight phenotype of Pink1B9

      (Malik et al., 2015)
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU suppresses the climbing defects and increased sensitivity to paraquat seen in park25 mutants. The flight defect is not suppressed.

      Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU does not suppress the climbing defects seen in Pink1B9 mutant flies. The flight defects seen in Pink1B9 mutants are enhanced.

      (Malik et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4da.PU/Vps35UAS.cMa rescues Vps35MH20/Vps35E42

      (Malik et al., 2015)
      Comments

      Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.cMa at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

      Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.

      Expression of Vps35Scer\UAS.cMa under the control of Scer\GAL4da.PU rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.

      (Malik et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Vps35Scer\UAS.cMa
      Vps35UAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Malik
      Secondary FlyBase IDs
        References (1)