FlyBase Allele Report: Dmel\N[GD14477]

General Information

Symbol

Dmel\N^GD14477

Species

D. melanogaster

Name

FlyBase ID

FBal0207692

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{GD14477}

Also Known As

UAS-Notch-RNAi, UAS-Notch RNAi, Notch-IR, Notch RNAi, N-IR, UAS-N-RNAi, UAS-N RNAi, UAS-N^RNAi, UAS-NotchRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14477}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14477

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Banreti et al., 2022)

model of colorectal cancer

CEA

(Zipper et al., 2022)

model of cancer

CEA

(Brás et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is exacerbated by Pcmt^GD8637

(Banreti et al., 2022)

is exacerbated by Pcmt^RNAi.UAS

(Banreti et al., 2022)

ameliorates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

model of colorectal cancer

is ameliorated by mir-277^UAS.cSa

(Zipper et al., 2022)

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

exacerbates cancer

modeled by Mps1^GL00184

(Brás et al., 2021)

modeled by Mps1^HMS01546

(Brás et al., 2021)

modeled by mad2^GLC01381

(Brás et al., 2021)

model of cancer

is exacerbated by Mps1^GL00184

(Brás et al., 2021)

is exacerbated by Mps1^HMS01546

(Brás et al., 2021)

is exacerbated by mad2^GLC01381

(Brás et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

increased occurrence of cell division | adult stage | female limited | somatic clone, with Scer\GAL4^NP7397

(Hudry et al., 2016)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived, with Scer\GAL4^elav.PU, Scer\GAL80^ple.PK

(Imai et al., 2015)

short lived, with Scer\GAL4^ple.PU

(Imai et al., 2015)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^bbg-C96

(Zhang et al., 2012)

visible, with Scer\GAL4^dpp.PU

(Zhang et al., 2012)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Under control conditions, the adulthood-only expression of N^GD14477 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to a significant increase in the adult posterior midgut mitotic index in females only, as compared to controls.

(Hudry et al., 2016)

Expression of N^GD14477 under the control of Scer\GAL4^ple.PU as well as the Scer\GAL4^elav.PU driver in combination with Scer\GAL80^ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan.

(Imai et al., 2015)

Expression of N^GD14477 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the age-dependent germline stem cell (GSC) loss seen in wild type females. Although a rapid decline in GSCs is seen at one week after clone induction, by two- to three weeks maintenance in knock-down females is better than that of wild type.

Expression of N^GD14477 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} slows female germline stem cell loss with age.

(Tseng et al., 2014)

Expression of N^GD14477 under the control of Scer\GAL4^bbg-C96 results in frequent serrations along the wing margin.

Expression of N^GD14477 under the control of Scer\GAL4^dpp.PU results in extra scutellar bristles.

(Zhang et al., 2012)

Adults expressing N^GD14477 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or late pupal lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 0-10% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 100% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% or 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14477

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^GD14477, Scer\GAL4^NP7397 has increased occurrence of cell division | somatic clone | female limited | adult stage phenotype, suppressible by Sxl^KK116156, Scer\GAL4^NP7397

(Hudry et al., 2016)

N^GD14477, Scer\GAL4^NP7397 has increased occurrence of cell division phenotype, suppressible by fzr^UAS.cSa, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of abnormal size | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

N^GD14477, Dcr-2^UAS.cDa, Scer\GAL4^wor.PA is a suppressor | partially of abnormal neuroanatomy phenotype of erm¹

(Weng et al., 2010)

NOT Suppressor of

Statement

Reference

N^GD14477/Scer\GAL4^Mhc.PW is a non-suppressor of visible | progressive phenotype of Pink1^B9

(Lee et al., 2013)

Other

Statement

Reference

N^GD14477, Scer\GAL4^NP7397, spi^s.UAS has hyperplasia | adult stage phenotype

(Hudry et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^GD14477, Scer\GAL4^NP7397 has adult posterior midgut epithelium | somatic clone | female limited phenotype, suppressible by Sxl^KK116156, Scer\GAL4^NP7397

(Hudry et al., 2016)

N^GD14477, Scer\GAL4^NP7397 has midgut phenotype, suppressible by fzr^UAS.cSa, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of endosome | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/N^GD14477 is a suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

N^GD14477, Dcr-2^UAS.cDa, Scer\GAL4^wor.PA is a suppressor | partially of larval neuroblast | ectopic | increased number phenotype of erm¹

(Weng et al., 2010)

NOT Suppressor of

Statement

Reference

N^GD14477/Scer\GAL4^Mhc.PW is a non-suppressor of wing | progressive phenotype of Pink1^B9

(Lee et al., 2013)

Other

Statement

Reference

N^GD14477, Scer\GAL4^NP7397, spi^s.UAS has adult posterior midgut epithelium | adult stage phenotype

(Hudry et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of N^GD14477 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) is suppressed by the co-expression of Sxl^KK116156.

The adulthood-only co-expression of N^GD14477 and spi^s.UAS under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to adult posterior midgut hyperplasia in females and males, as compared to controls.

(Hudry et al., 2016)

Expression of N^GD14477 under the control of Scer\GAL4^Mhc.PW does not suppress the abnormal wing posture of Pink1^B9 flies.

(Lee et al., 2013)

Inhibition of N activity through N^GD14477 suppresses numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation.

(Ouyang et al., 2011)

Expression of N^GD14477 (together with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^wor.PA in erm¹ larval brains leads to a dramatic reduction in ectopic type II neuroblasts compared to erm¹ brains alone.

(Weng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v27229

w¹¹¹⁸; P{GD14477}v27229

v27228

w¹¹¹⁸; P{GD14477}v27228/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14477

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^GD14477

N^IR

(Balmer et al., 2015)

N^RNAi

(Tseng et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (33)

Report Sections

Open Close