Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4ple.PU cells in adults during training blocks the increase in aversive olfactory 24 h memory observed when the recall environment better matches the training environment (presence of copper grid), compared to controls.
Activation of neurons expressing Scer\GAL4ple.PU through overexpression of temperature sensitive TrpA1 results in increased sleep loss in both young (1 day) and mature (7-8 day) flies compared to controls. The effects are similar in males and females and independent of day and night. No gross changes in dopamine cell anatomy are observed. When these neurons are activated for three days from day one after eclosion exhibit a reduced courtship index and severe reduction in copulation frequency at day 5. No effect on courtship is seen at day 12 when the neurons are activated for three days from day 7. The flies that are sleep-deprived when young, but not those sleep-deprived when mature, show a reduction in size of the VA1v fru[+] glomerulus caused by impaired post-eclosion growth. The volumes of the DA1, DL3 and VA1d glomeruli are unaffected.
Silencing of neurons expressing Scer\GAL4ple.PU through overexpression of shi results in significantly increased sleep in mature (7-8 day) flies compared to controls. A milder increase in sleep is seen in young (1 day old) flies).