FlyBase Allele Report: Dmel\GstO1[GD11995]

General Information

Symbol

Dmel\GstO1^GD11995

Species

D. melanogaster

Name

FlyBase ID

FBal0205158

Feature type

allele

Associated gene

Dmel\GstO1

Associated Insertion(s)

Carried in Construct

P{GD11995}

Also Known As

UAS-GstO1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11995}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11995

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Choi et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^da.PU

(Choi et al., 2019)

chemical sensitive, with Scer\GAL4^Ddc.PU

(Choi et al., 2019)

chemical sensitive, with Scer\GAL4^elav.PU

(Choi et al., 2019)

chemical sensitive, with Scer\GAL4^ple.PU

(Choi et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing GstO1^GD11995 under the control of Scer\GAL4^da.PU, Scer\GAL4^elav.PU, or Scer\GAL4^Ddc.PUx are significantly less resistant to ethanol sedation than controls: individuals stop climbing and succumb to sedation (loss of postural control) sooner than controls. A more modest defect is also observed upon Scer\GAL4^ple.PU-driven expression.

(Choi et al., 2019)

Adults expressing GstO1^GD11995 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development