FlyBase Allele Report: Dmel\Delta[GD2642]

General Information

Symbol

Dmel\Delta^GD2642

Species

D. melanogaster

Name

FlyBase ID

FBal0206865

Feature type

allele

Associated gene

Dmel\Delta

Associated Insertion(s)

Carried in Construct

P{GD2642}

Also Known As

UAS-Dl^RNAi, UAS-Dl-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2642}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2642

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

abnormal locomotor behavior, with Scer\GAL4^ple.PU

(Imai et al., 2015)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

decreased cell number, with Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Yang et al., 2013)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Wang et al., 2021)

increased cell number | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

long lived, with Scer\GAL4^ple.PU

(Imai et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Dl^GD2642 under the control of either Scer\GAL4^Mef2.PR or Scer\GAL4^elav-C155 has no significant effect on the number of abdominal lateral adult muscle precursor cells in mid-third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of Dl^GD2642 under the control of Scer\GAL4^ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly increased adult lifespan, improves climbing abilities of aged adult flies and the numbers of dopaminergic neurons in the PPL1, PPM1/2 and PPM3 clusters in the adult brain are significantly increased relative to controls. Expression under the control of Scer\GAL4^elav.PU in combination with Scer\GAL80^ple.PK (and tub-Gal80[ts] to limit the time of expression to adulthood) does not significantly affect adult survival.

(Imai et al., 2015)

Expression of Dl^GD2642 under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B) produces a decrease in the number of germline stem cell and cap cells in one week old female flies compared to controls.

(Yang et al., 2013)

Adults expressing Dl^GD2642 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing Dl^GD2642 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in a weak flyer phenotype or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 20-30% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 70-80% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2642

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Delta^GD2642, Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B has decreased cell number phenotype, non-enhanceable by Ser^GD14453, Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

Suppressor of

Statement

Reference

Delta^GD2642/Scer\GAL4^Tub.PU is a suppressor of increased size | somatic clone | larval stage phenotype of lin^A25

(Wen et al., 2024)

Delta^GD2642, Scer\GAL4^ple.PU is a suppressor | partially of decreased cell number | adult stage phenotype of Hsap\LRRK2^RG.UAS, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^GD2642, Scer\GAL4^ple.PU is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Hsap\LRRK2^RG.UAS, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^GD2642, Scer\GAL4^ple.PU is a suppressor | partially of abnormal flight phenotype of Hsap\LRRK2^RG.UAS, Scer\GAL4^ple.PU

(Imai et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Delta^GD2642, Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B has germline stem cell phenotype, non-enhanceable by Ser^GD14453, Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

Delta^GD2642, Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B has germarium cap cell phenotype, non-enhanceable by Ser^GD14453, Scer\GAL4^bab1-Gal4

(Yang et al., 2013)

Suppressor of

Statement

Reference

Delta^GD2642/Scer\GAL4^Tub.PU is a suppressor of wing disc | somatic clone | larval stage phenotype of lin^A25

(Wen et al., 2024)

Delta^GD2642, Scer\GAL4^ple.PU is a suppressor | partially of dopaminergic PPM1 neuron | adult stage phenotype of Hsap\LRRK2^RG.UAS, Scer\GAL4^ple.PU

(Imai et al., 2015)

Delta^GD2642, Scer\GAL4^ple.PU is a suppressor | partially of dopaminergic PPM2 neuron | adult stage phenotype of Hsap\LRRK2^RG.UAS, Scer\GAL4^ple.PU

(Imai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ser^GD14453 does not enhance the germline stem cell and cap cell loss seen when Dl^GD2642 is expressed under the control of Scer\GAL4^bab1-Gal4 (limited to the adult stages using Scer\GAL80^ts.αTub84B).

(Yang et al., 2013)

Xenogenetic Interactions

Statement

Reference

The loss of dopaminergic neurons from the PPM1/2 cluster in the adult brain and severe age-dependent decline in flight activity characteristic for flies expressing Hsap\LRRK2^RG.Scer\UAS under the control of Scer\GAL4^ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) is significantly suppressed by co-expression of Dl^GD2642.

(Imai et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v37287

w¹¹¹⁸; P{GD2642}v37287

v37288

w¹¹¹⁸; P{GD2642}v37288