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General Information
Symbol
Dmel\parkUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Shiba-Fukushima
FlyBase ID
FBal0302842
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dParkin
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild type park.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of parkScer\UAS.cSa in indirect flight muscles under the control of Scer\GAL4Mhc.PU shortens the mitochondria in the direction of the long axis compared with controls. ATP levels are similar to controls.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4Mhc.PU improves the decline in climbing ability usually seen in older flies, although performance in 7-day old flies is reduced compared to controls.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4ple.PU causes a reduction in the number of tubular mitochondria in the cell bodies of dopaminergic neurons in 5 day old flies compared to controls. The mitochondria usually observed outside of the cell body are not seen.

Flies expressing parkScer\UAS.cSa under the control of Scer\GAL4ple.PU do not exhibit reduced flying ability compared to controls.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4ple.PU results in an age-dependent loss of dopaminergic neurons. Lifespan is unaffected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The abnormally large, fused mitochondria observed in Pink1B9 mutant flies are completely rescued when parkScer\UAS.cSa is expressed under the control of Scer\GAL4Mhc.PU.

Expression of Pink1dsRNA.Scer\UAS suppresses the reduction in mitochondria length seen when parkScer\UAS.cSa is expressed in indirect flight muscles under the control of Scer\GAL4Mhc.PU.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4Mhc.PU suppresses the reduction in ATP levels seen in Pink1B9 mutant flies. The reduction in climbing ability and wing and thorax defects are also rescued.

Expression of parkScer\UAS.cSa suppresses the increase in mitochondrial length seen when Pink1dsRNA.Scer\UAS is expressed under control of Scer\GAL4Mhc.PU.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4ple.PU in a Pink1B9 mutant background rescues the mitochondrial aggregation and distribution defects seen with either mutation alone.

Pink1B9 partially suppresses the dopaminergic neuron loss seen when parkScer\UAS.cSa is expressed under the control of Scer\GAL4ple.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of parkScer\UAS.cSa under the control of Scer\GAL4da.PU rescues the mitochondria phenotype seen in parkΔ21/park1 mutant indirect flight muscles. The reduction in ATP levels is also rescued, and is improved to a level higher than that of controls.

Expression of parkScer\UAS.cSa under the control of Scer\GAL4Mhc.PU rescues the wing posture and thorax defects seen in parkΔ21/park1 mutant flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
parkScer\UAS.cSa
parkUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Shiba-Fukushima
Secondary FlyBase IDs
    References (4)