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General Information
Symbol
Dmel\Pink1dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0239190
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PINK1 RNAi, UAS-dPink1 RNAi, UAS-Pink1-RNAi, PINK1 RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Pink1 genomic/cDNA hybrid sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by cluGD13926
is exacerbated by cluKK108024
is exacerbated by rictorJF01370
is ameliorated by trcshort.UAS
is exacerbated by rictorΔ2
is ameliorated by rictorUAS.cHa
is ameliorated by trcL.UAS
is ameliorated by trcS292E.UAS.L
is exacerbated by Sin1unspecified
is exacerbated by rictorJF01086
is ameliorated by trcT453E.UAS.L
is exacerbated by Atg1K38Q.UAS
is ameliorated by Atg1UAS.cSa
is exacerbated by Atg13NIG.7331R
is exacerbated by Atg18aVDRC.cUa
is exacerbated by Atg3VDRC.cUa
is ameliorated by CatUAS.cUa
is ameliorated by GstS1UAS.cUa
is ameliorated by PHGPxUAS.cUa
is ameliorated by RpS9VDRC.cUa
is exacerbated by S6kSTDE.UAS
is exacerbated by S6kSTDETE.UAS
is exacerbated by S6kTE.UAS
is exacerbated by S6kUAS.cUa
is ameliorated by S6kVDRC.cUa
is ameliorated by Sod1UAS.cUa
is ameliorated by ThorUAS.cMa
is exacerbated by eIF4E1UAS.cRa
is ameliorated by parkUAS.Tag:HA
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Young (1-3 days) flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4tim.PE show defective rhythmicity of circadian locomotor activity under free-running (constant dark) conditions.

Expression of Pink1dsRNA.UAS under the control of Scer\GAL4Ddc.PL results in decreased lifespan and severely impaired adult locomotion, but Scer\GAL4GMR.PF-driven expression does not induce any eye morphology defects.

The expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PU leads to mitochondria fragmentation, despite of normal cristae morphology, in adult indirect flight muscles, as compared to controls.

Adult flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PU adults display wing posture defects, reduced jumping ability anf mitochondrial aggregation phenotype in muscles.

Expression of Pink1dsRNA.Scer\UAS driven by Scer\GAL4Mhc.PW increases abnormal wing posture compared to controls.

Down-regulation of Pink1, through expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW leads to mitochondrial degeneration.

Mitochondrial length is increased when Pink1dsRNA.Scer\UAS is expressed in indirect flight muscles under the control of Scer\GAL4Mhc.PU.

Aggregation of mitochondria is seen in the indirect flight muscles of animals expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW often have an abnormal wing posture and have reduced flight ability.

Expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW results in mitochondrial aggregation in muscle.

Expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW results in a drooped wing posture.

Expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4ple.PF in larval motor neurons results in increased anterograde flux and velocity of axonal mitochondrial transport, but no significant change in retrograde mitochondrial transport flux or velocity, increased mitochondrial length, and accumulation of mitochondria in the most distal boutons of neuromuscular junctions, as compared with controls.

Flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW often have an abnormal wing posture and have a reduced lifespan.

Prominent mitochondrial aggregates form in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4ple.PF, and in addition tubular mitochondrial structure are also seen.

The fraction of cultured dopaminergic neurons having thread-like tubular mitochondria is higher in cells expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4ple.PF than in control cells.

Adult flies still eclose after treatment with Pink1dsRNA.Scer\UAS, expressed under the control of Scer\GAL4da.G32. Both sexes exhibit either a held-up or a drooped wing posture, whereas control flies always hold their wings parallel to the body axis. The penetrance of this phenotype increases with age: When raised at 29[o]C, approximately 20% of newly eclosed flies exhibit abnormal wing posture, whereas by 7 days of age nearly 100% of them display this phenotype. These flies exhibit no problem with walking, but their climbing ability is greatly reduced and their ability to fly is completely abolished by 10 days of age.

Flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 display an approximate 70% reduction in overall ATP level compared with control flies. Raising the flies at 18[o]C until eclosion (so minimizing the RNAi effect), and then shifting them to 29[o]C immediately after eclosion (to induce a strong RNAi effect) results in an ATP level that initially is comparable with that of control flies, but within a week, the level of ATP drops sharply to approximately 40% of the control level. It remains low after 2 weeks under this experimental condition.

Flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 display a significantly reduced lifespan.

Expression of Pink1dsRNA.Scer\UAS, under the control of Scer\GAL4da.G32 results in severe disruption of the indirect flight muscles. Disrupted muscle integrity is found in both the wing elevator muscles (dorsal ventral muscles) and depressor muscles (dorsal longitudinal muscles).

Expression of Pink1dsRNA.Scer\UAS, under the control of Scer\GAL4Mhc.PW generates an abnormal wing posture and muscle disruption phenotype in approximately 40% of 7-day old flies raised at 29[o]C.

Expression of Pink1dsRNA.Scer\UAS, under the control of either Scer\GAL4da.G32 or Scer\GAL4Mhc.PW results in indirect flight muscles that exhibit irregular and dispersed myofibril arrangement. The number of mitochondria is reduced, whereas many of the remaining mitochondria are grossly swollen, lacking electron-dense material, and showing disintegration of cristae. Electron-dense deposits are found within the area of indirect flight muscles that succumb to severe disruption. Abnormally swollen mitochondria are present within morphologically normal myofibrils.

The tergotrochanteral muscles (TTMs) remain normal in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW or Scer\GAL4da.G32.

Expression of Pink1dsRNA.Scer\UAS, under the control of Scer\GAL4da.G32 induces extensive cell death (as visualised through TUNEL) in the indirect flight muscles at 4-days old, whereas age-matched controls flies do not show this. No cell death is observed in the indirect flight muscles in flies expressing Pink1dsRNA.Scer\UAS, under the control of Scer\GAL4Mhc.PW at 18[o]C. However, after shifting to 29[o]C continuously for 7 days, these flies exhibit extensive cell death.

Expression of Pink1dsRNA.Scer\UAS, under the control of Scer\GAL4ple.PF at 29[o]C results in a significant reduction of TH+ neurons in the lateral protocerebral posterior (PPL1) cluster (at 25-days old). The dorso-medial protocerebral cluster (PPM) also exhibits a modest reduction in neuronal number, whereas the other clusters are relatively unaffected. In newly eclosed flies, the dopamine content is comparable between control and Pink1dsRNA.Scer\UAS flies. As the flies age, both control and Pink1dsRNA.Scer\UAS flies exhibit age-dependent decline of dopamine levels. However, Pink1dsRNA.Scer\UAS flies consistently exhibit a more dramatic reduction than the control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Pink1dsRNA.UAS, Scer\GAL4Mhc.PW has short lived phenotype, suppressible by Keap1EY1/Keap1[+]

Pink1dsRNA.UAS, Scer\GAL4Mhc.PW has short lived phenotype, suppressible by Keap1EY5/Keap1[+]

NOT suppressed by
Statement
Reference

Pink1dsRNA.UAS, Scer\GAL4Mhc.PW has visible phenotype, non-suppressible by Keap1EY1/Keap1[+]

Pink1dsRNA.UAS, Scer\GAL4Mhc.PW has visible phenotype, non-suppressible by Keap1EY5/Keap1[+]

Enhancer of
Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The reduced adult lifespan along with the impaired climbing ability characteristic for flies expressing Pink1dsRNA.UAS under the control of Scer\GAL4Ddc.PL is ameliorated by co-expression of BuffyUAS.cQa. Adult flies expressing either Pink1dsRNA.UAS or BuffyUAS.cQa or both simultaneously under the control of Scer\GAL4GMR.PF do not show any eye morphology defects.

The mitochondria morphology defects in adult indirect flight muscles resulting from the expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PU are not enhanced by Chchd2H43 homozygosity.

The mitochondrial aggregations in flight muscles along with the wing posture and jumping ability defects observed in adult flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PU are significantly improved by co-expression of pumJF02267 but further enhanced by expression of Tim8JF01533, Tom40JF02030 or Tom20KK107626. The abnormal wing posture is also exacerbated by co-expression of Tim13KK108559 and the abnormal mitochondrial morphology in the flight muscles is partially suppressed by co-expression of any of the following: Pop2GD12804, AGO1TRiP.cUa, gwdsRNA.Scer\UAS.cUa, DCP1VDRC.cUa, DCP2HMS00115 or glodsRNA.Scer\UAS.cUa but worsened further by expression of eIF4G1HMS00762.

Co-expression of parkKK107919 or Atg1GD7149 or cluGD13926 or cluKK108024 increases the percentage of abnormal wing posture seen in flies with Pink1dsRNA.Scer\UAS driven by Scer\GAL4Mhc.PW.

Co-expression of Pink1dsRNA.Scer\UAS increases the percentage of abnormal wing posture seen in flies with cluKK108024 or cluGD13926 driven by Scer\GAL4Mhc.PW.

The mitochondrial degeneration seen in Pink1dsRNA.Scer\UAS mutants is not affected by down-regulation of ben (benGD1387).

Expression of Pink1dsRNA.Scer\UAS does not suppress the over-fragmentation of mitochondria seen when parkSE.Scer\UAS is expressed in indirect flight muscles under the control of Scer\GAL4Mhc.PU.

Expression of Pink1dsRNA.Scer\UAS suppresses the reduction in mitochondria length seen when parkScer\UAS.cSa is expressed in indirect flight muscles under the control of Scer\GAL4Mhc.PU.

Expression of parkSE.Scer\UAS suppresses the increase in mitochondrial length seen when Pink1dsRNA.Scer\UAS is expressed under control of Scer\GAL4Mhc.PU.

Expression of parkScer\UAS.cSa suppresses the increase in mitochondrial length seen when Pink1dsRNA.Scer\UAS is expressed under control of Scer\GAL4Mhc.PU.

Co-expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 suppresses the aggregation of mitochondria seen in the indirect flight muscles of animals expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Simultaneous co-expression of both Pink1dsRNA.Scer\UAS and Su(H)HM05110 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Simultaneous co-expression of both Pink1dsRNA.Scer\UAS and dmKK103869 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

The abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by rictorΔ2/Y and by Sin1unspecified/Sin1unspecified.

The abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by co-expression of either rictorJF01370 or rictorJF01086.

The abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is suppressed by co-expression of rictorScer\UAS.cHa.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is strongly suppressed by co-expression of rictorScer\UAS.cHa and moderately suppressed by co-expression of Sin1Scer\UAS.T:Zzzz\FLAG.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by rictorΔ2.

Co-expression of either trcshort.Scer\UAS, trcL.Scer\UAS, trcS292E.Scer\UAS or trcT453E.Scer\UAS suppresses the defects in flight ability caused by expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Co-expression of either trcK122A.Scer\UAS.T:Zzzz\FLAG or trcK122A+T453A.Scer\UAS enhances the defects in flight ability caused by expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Co-expression of either trcshort.Scer\UAS, trcL.Scer\UAS, trcS292E.Scer\UAS or trcT453E.Scer\UAS suppresses the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Co-expression of either trcK122A.Scer\UAS.T:Zzzz\FLAG or trcK122A+T453A.Scer\UAS enhances the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is suppressed by co-expression of either trcL.Scer\UAS or trcS292E.Scer\UAS.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by co-expression of trcK122A+T453A.Scer\UAS.

The ability of rictorΔ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of either MarfmiRNA.cUa.Scer\UAS or Atg1Scer\UAS.cSa.

The ability of rictorΔ2 to enhance the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is partially suppressed by co-expression of MiroKK102189.

The ability of trcK122A.Scer\UAS.T:Zzzz\FLAG to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of either MarfmiRNA.cUa.Scer\UAS or Atg1Scer\UAS.cSa.

The ability of trcK122A.Scer\UAS.T:Zzzz\FLAG to enhance the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is partially suppressed by co-expression of MiroKK102189.

Expression of APP-BP1Scer\UAS.cKa partially suppresses the drooped wing phenotype seen when Pink1dsRNA.Scer\UAS is expressed under the control of Scer\GAL4Mhc.PW.

Pgam5NP0568 significantly suppresses the abnormal wing posture of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW, but fails to suppress the short lifespan phenotype of these flies.

chicounspecified significantly suppresses the short lifespan phenotype of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW, but fails to suppress the abnormal wing posture of these flies.

The abnormal wing posture caused by expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is not suppressed Keap1EY1/+ or Keap1EY5/+, but the viability of aged flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is increased by Keap1EY1/+ or Keap1EY5/+.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\PARK2Scer\UAS.cYa completely blocks the ability of rictorΔ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Expression of Hsap\PARK2Scer\UAS.cYa completely blocks the ability of trcK122A+T453A.Scer\UAS to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW

Co-expression of Hsap\PINK1Scer\UAS.cYa with Pink1dsRNA.Scer\UAS, both under the control of Scer\GAL4Mhc.PW, suppresses the abnormal wing and disrupted muscle phenotypes induced by Pink1dsRNA.Scer\UAS.

Co-expression of Hsap\PINK1ΔC.Scer\UAS with Pink1dsRNA.Scer\UAS, both under the control of Scer\GAL4Mhc.PW, fails to suppress the abnormal wing and disrupted muscle phenotypes induced by Pink1dsRNA.Scer\UAS.

Mitochondrial integrity in Pink1dsRNA.Scer\UAS (under the control of either Scer\GAL4da.G32 or Scer\GAL4Mhc.PW) mutants is restored through co-expression of Hsap\PINK1Scer\UAS.cYa, but not co-expression of Hsap\PINK1ΔC.Scer\UAS.

Co-expression of Hsap\PINK1Scer\UAS.cYa but not Hsap\PINK1ΔC.Scer\UAS, with Pink1dsRNA.Scer\UAS (all under the control of Scer\GAL4ple.PF), is able to suppress the Pink1dsRNA.Scer\UAS dopaminergic neuron loss phenotype.

Co-expression of Hsap\PINK1Scer\UAS.cYa but not Hsap\PINK1ΔC.Scer\UAS, with Pink1dsRNA.Scer\UAS (all under the control of Scer\GAL4ple.PF), fully restores the level of dopamine in Pink1dsRNA.Scer\UAS mutants.

The abnormal wing postures caused by expression of Pink1dsRNA.Scer\UAS can be suppressed through co-expression of Hsap\PARK2Scer\UAS.cYa (both under the control of Scer\GAL4Mhc.PW), whereas co-expression of Hsap\PARK7Scer\UAS.cYa has no effect. This suppression also restores myofibril integrity and mitochondrial morphology. Overexpression of Hsap\PARK2Scer\UAS.cYa under the control of Scer\GAL4da.G32 is able to restore ATP levels in Pink1dsRNA.Scer\UAS flies, as well as restoring the number of TH+ neurons in the protocerebral posterior (PPL1) and dorso-medial protocerebral (PPM) clusters.While this overexpression exhibits a tendency to elevate brain dopamine levels in Pink1dsRNA.Scer\UAS animals, this effect is not statistically significant.

Complementation and Rescue Data
Comments

The expression of Pink1Scer\UAS.cYa, alongside Pink1dsRNA.Scer\UAS, both under the control of Scer\GAL4Mhc.PW, rescues the tissue-specific RNAi phenotype.

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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pink1dsRNA.Scer\UAS
Pink1dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (22)