FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mTorWT.UAS.Tag:FLAG
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General Information
Symbol
Dmel\mTorWT.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0141341
Feature type
allele
Associated gene
Dmel\mTor
Associated Insertion(s)
Carried in Construct
P{UAS-mTor.WT}
Also Known As
UAS-Tor, UAS-TORWT, UAS-TOR.WT, UAS-dTORWT
Key Links
Transgenic product class
wild_type
(Hennig and Neufeld, 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Hennig and Neufeld, 2002)
Mutagen
in vitro construct
(Hennig and Neufeld, 2002)
Progenitor genotype
Carried in construct
P{UAS-mTor.WT}
Cytology
Description

UASt regulatory sequences drive expression of full length Tor tagged at the N-terminal end with Tag:FLAG.

(Hennig and Neufeld, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mTor
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  neuronal ceroid lipofuscinosis
      modeled by cathD1
      (Kuronen et al., 2009)
      ameliorates  Parkinson's disease 6
      modeled by Pink1RNAi.UAS.cYa
      (Liu and Lu, 2010)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal developmental rate, with Scer\GAL4Bx-MS1096

      (Hennig and Neufeld, 2002)

      abnormal developmental rate, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      abnormal neurophysiology | larval stage, with Scer\GAL4G14

      (Penney et al., 2012)

      abnormal neurophysiology | larval stage, with Scer\GAL4Mhc.PW

      (Penney et al., 2012)

      abnormal neurophysiology | third instar larval stage, with Scer\GAL4Mhc.PW

      (Penney et al., 2016)

      abnormal size, with Scer\GAL4ey.PH

      (Wang and Huang, 2009)

      abnormal size, with Scer\GAL4nub-AC-62

      (Resnik-Docampo and de Celis, 2011)

      abnormal size | adult stage, with Scer\GAL4Bx-MS1096

      (Hennig and Neufeld, 2002)

      abnormal size | P-stage, with Scer\GAL4NP0001

      (Xu et al., 2015)

      decreased body size, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      decreased cell number, with Scer\GAL4Bx-MS1096

      (Goberdhan et al., 2005)

      decreased cell number, with Scer\GAL4en-e16E

      (Goberdhan et al., 2005)

      decreased cell size | larval stage, with Scer\GAL4Act5C.PP

      (Hennig and Neufeld, 2002)

      decreased occurrence of cell division | larval stage, with Scer\GAL4Act5C.PP

      (Hennig and Neufeld, 2002)

      increased body size | larval stage, with Scer\GAL4Cg.PU

      (Zheng et al., 2019)

      increased cell death, with Scer\GAL4en.PU

      (Resnik-Docampo and de Celis, 2011)

      increased cell size, with Scer\GAL4en-e16E

      (Goberdhan et al., 2005)

      increased occurrence of cell division | larval stage, with Scer\GAL4en.PU

      (Resnik-Docampo and de Celis, 2011)

      lethal, with Scer\GAL4Act5C.PI

      (Hennig and Neufeld, 2002)

      lethal, with Scer\GAL4ap-md544

      (Hennig and Neufeld, 2002)

      lethal, with Scer\GAL4da.G32

      (Kapahi et al., 2004)

      lethal, with Scer\GAL4ey.PH

      (Hennig and Neufeld, 2002)

      visible, with Scer\GAL4Bx-MS1096

      (Hennig and Neufeld, 2002)

      visible, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      visible | larval stage, with Scer\GAL4Cg.PU

      (Zheng et al., 2019)

      wild-type, with Scer\GAL4GMR.PF

      (Hennig and Neufeld, 2002)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TorWT.UAS.Tag:FLAG under the control of Scer\GAL4Cg.PU leads to an increase in the animal body size at larval stage on the low protein diet when compared to controls.

      (Zheng et al., 2019)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mhc.PW does not result in any significant difference in amplitude of miniature excitatory junction currents (mEJCs), but does lead to an increase in amplitude of EJCs, and an increase in quantal content of larval neuromuscular junctions, as compared to controls.

      (Penney et al., 2016)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Myo31DF-NP0001 results in delayed midgut degradation after pupariation, with significantly increased size of the gastric caeca 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

      (Xu et al., 2015)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4en.PU results in a significant increase in the fraction of mitotic cells in the posterior compartment of the wing disc.

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4en.PUinduces only very low levels of apoptosis in the the wing disc.

      Over-expression of TorWT.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4nub-AC-62 causes a strong and variable phenotype of wing size reduction.

      (Resnik-Docampo and de Celis, 2011)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH results in a small reduction in eye size at 25[o]C, and a greater reduction in eye size at 29[o]C.

      (Wang and Huang, 2009)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF produces no obvious eye phenotype.

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG throughout the developing adult wing, driven by Scer\GAL4Bx-MS1096, reduces wing growth.

      The posterior wing cells are enlarged in adult flies that express TorWT.Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4en-e16E, despite the reduction in overall compartment size.

      (Goberdhan et al., 2005)

      TorWT.Scer\UAS.T:Zzzz\FLAG; Scer\GAL4ppl.PP flies have increased triglyceride levels (normalised to body weight) than wild-type.

      (Teleman et al., 2005)

      Animals expressing TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 do not eclose to adulthood.

      (Kapahi et al., 2004)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Bx-MS1096 results in smaller wings than normal which show an upward curvature. The flies show a developmental delay. Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4en-e16E results in a developmental delay and a reduced posterior wing. Wing disc cells expressing TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PP are smaller and an increased proportion of the cells have a 2C DNA content (indicating a delay or arrest in the G1 phase of the cell cycle) compared to wild-type control cells. The mean proliferation rate of the cells is approximately half that of the control cells. Clones expressing TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PP are also recovered less frequently than wild-type clones.

      (Hennig and Neufeld, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression (at 18[o]C for low level expression) of TorWT.Scer\UAS.T:Zzzz\FLAG driven in the fat body by Scer\GAL4cg.PU suppresses the autophagy phenotype of wcyA/wcyA clones; expression driven in the retina by Scer\GAL4ninaE.PU partially significantly suppresses the increased number of autophagosomes, amphisomes, autolysosomes and lysosomes seen in wcyA/wcyA clones, along with improving synaptic transmission (significantly increasing off transient amplitude).

      (David-Morrison et al., 2016)

      Lrrke03680/+ suppresses the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mhc.PW.

      (Penney et al., 2016)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.

      (Rallis et al., 2013)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 enhances by 2-10-fold the level of vacuolar change in cathD1 mutant retina.

      (Kuronen et al., 2009)

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH in a Lfee background enhances the reduction in eye size observed in Lfee animals.

      Co-expression of dmScer\UAS.cZa suppresses the reduction in eye size caused by expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH.

      (Wang and Huang, 2009)

      When cooverexpressed with either pathGS13857 or CG1139GS10666, under the control of Scer\GAL4GMR.PF, TorWT.Scer\UAS.T:Zzzz\FLAG significantly enhances the overgrowth phenotype induced by either of these transporters alone.

      pathGS13857 , pathScer\UAS.cGa and CG1139GS10666 all significantly enhance the phenotype produced by overexpressing TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Bx-MS1096.

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG does not affect the Scer\GAL4GMR.PF>foxoGS9928 phenotype.

      Co-expression of pathScer\UAS.cGa and TorWT.Scer\UAS.T:Zzzz\FLAG together enhance the Scer\GAL4GMR.PF>foxoGS9928 reduced eye phenotype.

      (Goberdhan et al., 2005)

      TorWT.Scer\UAS.T:Zzzz\FLAG; Scer\GAL4ppl.PP does not suppress the reduced triglyceride levels per unit of body weight seen in Df(3L)meltΔ1 homozygous flies.

      (Teleman et al., 2005)

      Coexpression of TorWT.Scer\UAS.T:Zzzz\FLAG and Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in a severe reduction in wing size.

      (Hennig and Neufeld, 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      mTorWT.UAS.Tag:FLAG/Scer\GAL4DIP-γ-MI03222-GAL4 rescues mTorΔP

      (Luo et al., 2020)

      mTorWT.UAS.Tag:FLAG/Scer\GAL4Mhc.PW rescues mTorE161K/mTorΔP

      (Penney et al., 2012)
      Partially rescues

      Scer\GAL4Act5C.PI/mTorWT.UAS.Tag:FLAG partially rescues mTorunspecified

      (Hennig and Neufeld, 2002)
      Comments

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI rescues Torunspecified null animals (which usually arrest as small second instar larvae) to the pupal stage, but the rescued animals fail to survive to adulthood.

      (Hennig and Neufeld, 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "Flag-Tor", transfected into S2 cells.

      (Gao et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TorWT.Scer\UAS.T:Zzzz\FLAG
      TorWT.UAS.Tag:FLAG
      mTorWT.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (33)