FlyBase Allele Report: Dmel\brp[69]

General Information

Symbol

Dmel\brp⁶⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0194770

Feature type

allele

Associated gene

Dmel\brp

Associated Insertion(s)

Carried in Construct

Also Known As

brp69

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Kittel et al., 2006)

Progenitor genotype

P{SUPor-P}KG03268

(Kittel et al., 2006)

P{SUPor-P}brp^KG04653b

(Kittel et al., 2006)

Cytology

Description

Transmobilization of elements P{SUPor-P}KG04653b and P{SUPor-P}KG03268 resulting in the deletion of most of the brp gene, leaving only the 5' regions, exon 1, exon 2 and part of exon 3.

(Kittel et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:9,515,923..9,540,617 [+]

Comment:

A deletion of brp sequences caused by mobilization of the P{SUPor-P}KG03268 and P{SUPor-P}brp^KG04653b elements within the gene.

(Fouquet et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

brp^nude/brp⁶⁹ larvae have significantly reduced locomotion, compared to controls; they also have significantly reduced numbers of synaptic vesicles around motor neuron T-bars and significantly reduced NMJ presynaptic active zone area, compared to controls, with no effect on the number of presynaptic active zones; there is no effect on the rate of miniature excitatory postsynaptic currents (minis) in muscle 6 at the NMJ or on excitatory postsynaptic current (eEPSC) amplitude or decay time constant, compared to controls, but paired-pulse depression of eEPSCs is significantly increased, compared to controls.

brp⁶⁹/+ larvae have significantly increased locomotion, compared to controls.

(Scholz et al., 2019)

brp^Δ190/brp⁶⁹ flies have reduced fitness and eclosion rates compared to controls.

(Matkovic et al., 2013)

T bars are missing at the active zones of the neuromuscular junction in mutant third instar larvae.

(Fouquet et al., 2009)

brp⁶⁹/Df(2R)BSC29 mutants have slightly smaller NMJs and fewer individual synapses than wild-type larvae. However, individual receptor fields are enlarged in the mutants. In the mutant NMJs, postsynaptic densities appear larger than in wild type and there are intermittent rufflings of the presynaptic membrane. Notably, the brp⁶⁹/Df(2R)BSC29 NMJs completely lack T-bars, but occasionally show a little residual electron-dense material attached to the active zone membrane.

The average miniature excitatory junctional currents (mEJC) amplitude is increased in brp⁶⁹/Df(2R)BSC29 mutants, while the frequency is not significantly altered. Quantal content of mutant NMJs is significantly decreased compared to controls. Although the rise time of eEJCs is significantly increased at mutant NMJs, the rise time of mEJCs is the same as controls. The decay time constant of eEJCs is not significantly altered at brp⁶⁹/Df(2R)BSC29 synapses, while the decay time constant of mEJCs decay is longer in the mutant than in the control.

A 10Hz stimulation of NMJs reveals transient short term facilitation of brp⁶⁹/Df(2R)BSC29 currents and the absence of a frequency-dependent depression of steady-state current amplitudes compared with controls. Paired-pulse protocols applied to the NMJ do not produce marked facilitation at control synapses, while there is a prominent facilitation at mutant NMJs, showing that vesicle release at brp⁶⁹/Df(2R)BSC29 synapses benefits from accumulation of Ca²⁺. After bath application of membrane-permeable EGTA-AM, the reduction of evoked vesicle release is more pronounced in mutants than in wild type. Additionally, there are a reduced number of Ca²⁺ channels over the entire NMJ and at synapses in brp⁶⁹/Df(2R)BSC29 mutants compared to controls. These observations suggest that impaired vesicle release in the mutants is caused by mislocalization of presynaptic Ca²⁺ channels.

(Kittel et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

brp⁶⁹/brp^nude has abnormal locomotor behavior | larval stage phenotype, enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has abnormal neurophysiology | larval stage phenotype, enhanceable by Scer\GAL4^RapGAP1-OK6/cpx^UAS.RU.EGFP

(Scholz et al., 2019)

NOT Enhanced by

Statement

Reference

brp⁶⁹/brp^nude has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

NOT suppressed by

Statement

Reference

brp⁶⁹/brp^nude has abnormal neuroanatomy | larval stage phenotype, non-suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

NOT Enhancer of

Statement

Reference

brp⁶⁹/brp^nude is a non-enhancer of abnormal locomotor behavior | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Suppressor of

Statement

Reference

brp⁶⁹ is a suppressor of abnormal neurophysiology | larval stage phenotype of Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2}

(Mushtaq et al., 2022)

brp⁶⁹/brp^nude is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp[+] is a suppressor of abnormal neurophysiology | larval stage phenotype of Scer\GAL4^G14, mTor^{WT.UAS.Tag:FLAG}

(Penney et al., 2012)

NOT Suppressor of

Statement

Reference

brp⁶⁹/brp^nude is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Other

Statement

Reference

Df(3R)Exel7283/Madm^4S3, Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2}, brp⁶⁹ has abnormal neuroanatomy | larval stage phenotype

(Mushtaq et al., 2022)

Df(3R)Exel7283/Madm^4S3, Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2}, brp⁶⁹ has abnormal neurophysiology | larval stage phenotype

(Mushtaq et al., 2022)

brp⁶⁹, cpx[+]/cpx¹²⁵⁷ has abnormal locomotor behavior | larval stage phenotype

(Scholz et al., 2019)

brp⁶⁹/brp^nude, cpx^SH1/cpx¹²⁵⁷ has abnormal neurophysiology | larval stage phenotype

(Scholz et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

brp⁶⁹/brp^nude has embryonic/larval neuromuscular junction phenotype, enhanceable by Scer\GAL4^RapGAP1-OK6/cpx^UAS.RU.EGFP

(Scholz et al., 2019)

brp⁶⁹/brp^nude has muscle cell of A1-7 ventral longitudinal muscle 3 phenotype, enhanceable by Scer\GAL4^RapGAP1-OK6/cpx^UAS.RU.EGFP

(Scholz et al., 2019)

NOT Enhanced by

Statement

Reference

brp⁶⁹/brp^nude has synaptic vesicle | larval stage phenotype, non-enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has T-bar | larval stage phenotype, non-enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has presynaptic active zone | larval stage phenotype, non-enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has embryonic/larval motor neuron phenotype, non-enhanceable by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

NOT suppressed by

Statement

Reference

brp⁶⁹/brp^nude has synaptic vesicle | larval stage phenotype, non-suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has T-bar | larval stage phenotype, non-suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has presynaptic active zone | larval stage phenotype, non-suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude has embryonic/larval motor neuron phenotype, non-suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

NOT Enhancer of

Statement

Reference

brp⁶⁹/brp^nude is a non-enhancer of synaptic vesicle | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude is a non-enhancer of T-bar | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Suppressor of

Statement

Reference

brp⁶⁹ is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Madm^UAS.EGFP, Scer\GAL4^{GluRIIB-MI03631-TG4.2}

(Mushtaq et al., 2022)

brp⁶⁹/brp^nude is a suppressor of presynaptic active zone | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude is a suppressor | partially of embryonic/larval motor neuron phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp[+] is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^G14, mTor^{WT.UAS.Tag:FLAG}

(Penney et al., 2012)

NOT Suppressor of

Statement

Reference

brp⁶⁹/brp^nude is a non-suppressor of synaptic vesicle | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

brp⁶⁹/brp^nude is a non-suppressor of T-bar | larval stage phenotype of cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

brp⁶⁹/+; cpx¹²⁵⁷/+ double heterozygous larvae have significantly reduced locomotion, compared to controls, unlike either heterozygote alone.

brp^nude/brp⁶⁹; cpx¹²⁵⁷/cpx^SH1 double mutants have suppression of the increased eEPSC amplitude and mini frequency of cpx¹²⁵⁷/cpx^SH1 mutant larval muscle 6 and enhancement of the paired-pulse depression seen in both brp^nude/brp⁶⁹ mutants and cpx¹²⁵⁷/cpx^SH1 mutants.

(Scholz et al., 2019)

Heterozygosity for brp⁶⁹ results in a significant reduction in the increase in quantal content (QC) normally observed in homozygous Df(2R)KT40 mutant larvae.

(Tsurudome et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

brp⁶⁹/Df(2R)BSC29 is rescued by Scer\GAL4^RapGAP1-OK6/brp^{D1-4.UAS.EGFP}

(Fouquet et al., 2009)

Partially rescued by

brp⁶⁹/Df(2R)BSC29 is partially rescued by Scer\GAL4^RapGAP1-OK6/brp^UAS.cWa

(Kittel et al., 2006)

brp⁶⁹/Df(2R)BSC29 is partially rescued by Scer\GAL4^elav-C155/brp^UAS.cWa

(Kittel et al., 2006)

Not rescued by

brp⁶⁹/Df(2R)BSC29 is not rescued by Scer\GAL4^RapGAP1-OK6/brp^{D1-3.UAS.EGFP}

(Fouquet et al., 2009)

brp⁶⁹/Df(2R)BSC29 is not rescued by Scer\GAL4^RapGAP1-OK6/brp^{D2-4.UAS.EGFP}

(Fouquet et al., 2009)

Comments

Expression of brp^{D1-3.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Rapgap1-OK6 fails to rescue the formation of T bars at the neuromuscular junction in brp⁶⁹/Df(2R)BSC29 third instar larvae.

Expression of brp^{D1-4.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Rapgap1-OK6 rescues the formation of T bars at the neuromuscular junction in brp⁶⁹/Df(2R)BSC29 third instar larvae.

Expression of brp^{D2-4.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Rapgap1-OK6 fails to rescue the formation of T bars at the neuromuscular junction in brp⁶⁹/Df(2R)BSC29 third instar larvae, although small electron-dense aggregates are often seen localised to the active zone membrane.

(Fouquet et al., 2009)

Expression of the brp^Scer\UAS.cWa transgene, under the control of either Scer\GAL4^OK6 or Scer\GAL4^elav-C155, rescues the larval lethality of brp⁶⁹/Df(2R)BSC29 mutants. In addition, Scer\GAL4^OK6>brp^Scer\UAS.cWa expression partially restores the lack of T-bars seen in these mutants, although T-bars in the rescued flies are somewhat aberrant in shape. Expression of the transgene with both drivers can partially rescue the drop in current amplitude from brp⁶⁹/Df(2R)BSC29 NMJs.

(Kittel et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Brp⁶⁹

(Cheng et al., 2011)

brp69

(Ghelani et al., 2023, Peled et al., 2014)

brp⁶⁹

(Medeiros et al., 2024, Cunningham et al., 2022, Mushtaq et al., 2022, Driller et al., 2019, Scholz et al., 2019, Akbergenova et al., 2018, Migh et al., 2018, Siebert et al., 2015, Matkovic et al., 2013, Penney et al., 2012, Owald et al., 2010, Fouquet et al., 2009, Johnson et al., 2009, Schmid et al., 2008, Kittel et al., 2006, Kittel et al., 2006)

brp^DF(2R)69

(Driller et al., 2019)

brp^Df69

(Driller et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (20)

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